Effects of boric-acid-applied green tea on the expressions of heat shock proteins in MCF-7 cells

Melike Ersöz; Ayşe Karatuğ Kaçar; İşıl Sezekler; Zeynep Mine Coşkun

doi:10.17826/cumj.435450

TR EN

MCF-7 hücrelerinde borik asit uygulanan yeşil çayın ısı şok proteinlerinin ekspresyonlarına etkileri

Öz

Amaç: Çalışmanın amacı, farklı konsantrasyonlarda borik asit uygulanan yeşil çayın MCF-7 hücrelerinde ısı şoku proteini (HSP) 70 ve HSP 90 üzerine etkilerini incelemektir.

Gereç ve Yöntem: Camelia sinensis (yeşil çay)’in yetiştirildiği toprağa 0 mg/m2 (grup 1), 100 mg/m2 (grup 2), 300 mg/m2 (grup 3) ve 500 mg/m2 (grup 4) borik asit sodyum tetraborat tamponu içerisinde uygulandı. Toplanan yeşil çay yaprakları kurutuldu ve 40 dakika boyunca 80° C'de suda demlendi. Sitotoksisite MTT yöntemi ile analiz edildi. Hazırlanan yeşil çay ekstraktları (250 μg/ml) MCF-7 hücrelerine uygulandı. HSP 70 ve HSP 90'ın mRNA ekspresyonları qRT-PZR ile HSP 70 ve HSP 90 protein seviyeleri Western blotting yöntemi ile belirlendi.

Bulgular: 100 ve 500 mg/m2 borik asit uygulanan yeşil çay ekstraktları MCF-7 hücrelerinde HSP 90 mRNA ekspresyonunu borik asit içermeyen yeşil çay ekstraktına göre arttırdı. Borik asit uygulanmış yeşil çay ekstraktı ve borik asit içermeyen yeşil çay ekstraktının MCF-7 hücrelerindeki HSP 70 ve HSP 90 protein seviyeleri arasında herhangi bir değişiklik saptanmadı.

Sonuç: Bulgularımıza göre, MCF-7 hücrelerinde HSP 70 ve HSP 90'ın protein ekspresyonları borik asit uygulanmış ve uygulanmamış yeşil çay ekstraktları ile değişmedi.

Anahtar Kelimeler

MCF-7 hücreleri,ısı şok protein,yeşil çay,borik asit,sitotoksisite

Effects of boric-acid-applied green tea on the expressions of heat shock proteins in MCF-7 cells

Abstract

Purpose: The study aims to examine the effects of boric acid at different concentrations applied to green tea on heat shock protein (HSP) 70 and HSP 90 in MCF-7 cells.

Materials and Methods: Boric acid in sodium tetraborate buffer of 0 mg/m2 (group 1), 100 mg/m2 (group 2), 300 mg/m2 (group 3), and 500 mg/m2 (group 4) were applied to soil where Camelia sinensis (green tea) grown. The collected green tea leaves were dried and brewed in water at 80°C for 40 minutes. Cytotoxicity was analyzed by MTT method. The prepared green tea extracts (250 μg/ml) were applied to MCF-7 cells. The mRNA expressions of HSP 70 and HSP 90 were determined by qRT-PCR. The HSP 70 and HSP 90 protein levels were measured by Western blotting method.

Results: 100 and 500 mg/m2 boric-acid-applied green tea extracts increased HSP 90 mRNA expressions in MCF-7 cells compared to green tea extract without boric acid. HSP 70 and HSP 90 protein levels did not show any change between MCF-7 cells with boric-acid-applied green tea extract and green tea extract without boric acid.

Conclusion: According to our results, the protein expressions of HSP 70 and HSP 90 in MCF-7 cells changed by neither boric-acid-applied green tea extracts nor green tea extracts without any boric-acid-applied.

Keywords

MCF-7 cells,heat shock protein,green tea,boric acid

Kaynakça

REFERENCES1. World Health Organization (WHO). http://www.who.int (accessed Feb 2018).
2. American Cancer Society. https://www.cancer.org (accessed Feb 2018).
3. Lee YH, Jang YH, Kim YS, Kim J, Seong BL. Evaluation of green tea extract as a safe personal hygiene against viral infections. J Biol Eng. 2018;12:1.
4. Forester SC, Lambert JD. Antioxidant effects of green tea. Mol Nutr Food Res. 2011;55(6):844–54.
5. Han DH, Jeong JH, Kim JH. Anti-proliferative and apoptosis induction activity of green tea polyphenols on human promyelocytic leukemia HL-60 cells. Anticancer Res. 2009;29(4):1417-21.
6. Bimonte S, Cascella M, Leongito M, Palaia R, Caliendo D, Izzo F, et al. An overview of pre-clinical studies on the effects of (-)-epigallocatechin-3-gallate, a catechin found in green tea, in treatment of pancreatic cancer. Recenti Prog Med. 2017;108(6):282-7.
7. Herera-Rodrigez M, González-Fontes A, Rexach J, Camacho-Cristobal JJ, Maldonado JM, Narrova-Gochicoa MT. Role of boron in vascular plants and respononse mechanisms to boron stresses. Plant Stress. 2010;4(2):115-22.
8. Barranco WT, Hudak PF, Eckhert CD. Evaluation of ecological and in vitro effects of boron on prostate cancer risk (United States). Cancer Causes Control. 2007;18(1):71-7.
9. Das BC, Thapa P, Karki R, Schinke C, Das S, Kambhampati S, et al. Boron chemicals in diagnosis and therapeutics. Future Med Chem. 2013;5(6):653-76.
10. Lianos GD, Alexiou GA, Mangano A, Mangano A, Rausei S, Boni L, et al. The role of heat shock proteins in cancer. Cancer Lett. 2015;360(2):114-8.

11. Saini J, Sharma PK. Clinical, prognostic and therapeutic significance of heat shock proteins in cancer. Curr Drug Targets. 2017;doi:10.2174/ 1389450118666170823121248 (in press).
12. Alexiou GA, Vartholomatos G, Stefanaki K, Patereli A, Dova L, Karamoutsios A, et al. Expression of heat shock proteins in medulloblastoma, J Neurosurg Pediatr. 2013;12(5):452-7.
13. Pfaffl MW. A new mathematical model for relative quantification in real-time RT- PCR. Nucleic Acids Res. 2001;29(9):e45.
14. Comsa Ş, Cîmpean AM, Raica M. The story of MCF-7 breast cancer cell line: 40 years of experience in research. Anticancer Res. 2015;35(6):3147-54.
15. Holliday DL, Speirs V. Choosing the right cell line for breast cancer research. Breast Cancer Res. 2011;13(4):215.
16. Yuan JM, Sun C, Butler LM. Tea and cancer prevention: epidemiological studies. Pharmacol Res. 2011;64(2):123-35.
17. Ogunleye AA, Xue F, Michels KB. Green tea consumption and breast cancer risk or recurrence: a meta-analysis. Breast Cancer Res Treat. 2010;119(2):477-84.
18. Zhang D, Al-Hendy M, Richard-Davis G, Montgomery-Rice V, Sharan C, Rajaratnam V, et al. Green tea extract inhibits proliferation of uterine leiomyoma cells in vitro and in nude mice. Am J Obstet Gynecol. 2010;202(3):1-9.
19. Liu XL, Liu HQ, Li J, Yang L, Zhao X. Experimental study on anti-tumor effect and mechanism of green tea extract. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014;34(11):1369-73.
20. Becker L, Scheffczyl A, Förster B, Oehlmann J, Princz J, Römbke J, et al. Effects of boric acid on various microbes, plants, and soil invertebrates. J Soils and Sediments. 2011;11(2):238-48.
21. Sogut I, Paltun SO, Tuncdemir M, Ersoz M, Hurdag C. The antioxidant and antiapoptotic effect of boric acid on hepatoxicity in chronic alcohol-fed rats. Can J Physiol Pharmacol. 2018;96(4):404-11.
22. Cesa LC, Shao H, Srinivasan SR, Tse E, Jain C, Zuiderweg ERP, et al. X-linked inhibitor of apoptosis protein (XIAP) is a client of heat shock protein 70 (Hsp70) and a biomarker of its inhibition. J Biol Chem. 2018;293(7):2370-80.
23. Khan GN, Kim EJ, Shin TS, Lee SH. Azacytidine-induced chemosensitivity to doxorubicin in human breast cancer MCF7 Cells. Anticancer Res. 2017;37(5):2355-64.
24. Jagadish N, Agarwal S, Gupta N, Fatima R, Devi S, Kumar V, et al. Heat shock protein 70-2 (HSP70-2) overexpression in breast cancer. J Exp Clin Cancer Res. 2016;35(1):150.
25. Forsberg LK, Liu W, Holzbeierlein J, Blagg BSJ. Modified biphenyl Hsp90 C-terminal inhibitors for the treatment of cancer. Bioorg Med Chem Lett. 2017;27(18):4514-9.
26. Moses MA, Henry EC, Ricke WA, Gasiewicz TA. The heat shock protein 90 inhibitor, (-)-epigallocatechin gallate, has anticancer activity in a novel human prostate cancer progression model. Cancer Prev Res (Phila). 2015;8(3):249-57.
27. Li Y, Zhang T, Jiang Y, Lee HF, Schwartz SJ, Sun D. (-)-Epigallocatechin-3-gallate inhibits Hsp90 function by impairing Hsp90 association with cochaperones in pancreatic cancer cell line Mia Paca-2. Mol Pharm. 2009;6(4):1152-9.
28. Haseeb K, Wang J, Xiao K, Yang KL, Sun PP, Wu XT, et al. Effects of boron supplementation on expression of Hsp70 in the spleen of African Ostrich. Biol Trace Elem Res. 2017;182(2):317-27.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Melike Ersöz Bu kişi benim
0000-0002-5289-5809
Türkiye

Ayşe Karatuğ Kaçar
0000-0001-6032-470X
Türkiye

İşıl Sezekler Bu kişi benim
0000-0001-8544-1195
Türkiye

Zeynep Mine Coşkun ^*
0000-0003-4791-6537
Türkiye

Yayımlanma Tarihi

31 Mart 2019

Gönderilme Tarihi

21 Haziran 2018

Kabul Tarihi

20 Ağustos 2018

Yayımlandığı Sayı

Yıl 2019 Cilt: 44 Sayı: 1

DOI

https://doi.org/10.17826/cumj.435450

IZ

https://izlik.org/JA37HF22AB

MLA

Ersöz, Melike, vd. “Effects of boric-acid-applied green tea on the expressions of heat shock proteins in MCF-7 cells”. Cukurova Medical Journal, c. 44, sy 1, Mart 2019, ss. 66-71, doi:10.17826/cumj.435450.