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Sıçanlarda immünsupresyonsuz xenotransplantasyon uygulamasının etkililiği

Year 2019, Volume: 44 Issue: 3, 782 - 787, 30.09.2019
https://doi.org/10.17826/cumj.486224

Abstract

Amaç: Çalışmamızın amacı, insan paratiroid dokularından izole edilen hiperplazik hücrelerin, sıçanlara immünsupresan verilmeden transplante edilerek transplantasyon verimliliğinin incelenmesidir. 

Gereç ve Yöntem: Çalışmada, paratiroid hiperplazi tanısıyla opere edilen 62 yaşında erkek hastadan izole edilen hiperplazik paratiroid hücreleri kullanıldı. Alıcı olarak üç adet erkek Sprague Dawley sıçan kullanıldı. İnsandan elde edilen hücreler sıçanlara immünsupresan uygulanmadan transplante edildi. Transplantasyon işlemi sırasında hücreler doğrudan sırasıyla, vena portaya, vena cavaya ve periferik vene enjekte edildi. Transplantasyon başarısının takibi açısından total parathormon (PTH) ve serum kalsiyum düzeyleri, transplantasyon öncesinde ve sonrasında ölçüldü.

Bulgular: Sıçanların transplantasyon öncesi total PTH  düzeyleri 1,4-4,2 pg/mL aralığında, 45. gün değerleri ise 6,2-17,1 pg/mL aralığında tespit edildi. 

Sonuç: Periferik dolaşımdan ve vena portadan yapılan transplantasyonun, vena cavaya göre daha başarılı olduğu belirlendi. Sonuç olarak nakil işleminde periferik venden hücre naklinin yararlı olabileceği düşünüldü. Sunduğumuz çalışmanın ilerleyen dönemlerde hayvan sayısı arttırılarak tekrarlanması araştırmayı kuvvetlendirecektir.


References

  • 1. Shoback D. Clinical practice. Hypoparathyroidism. N Engl J Med. 2008;359:391–403.
  • 2. Leem TH, Volpi E, Eisele DW. Non-neural complications of thyroid and parathyroid surgery. In: Randolph GE.Surgery of the thyroid and parathyroid, 2nd edition Philadelphia: Elsevier Saunders, 2013;446-542.
  • 3. Agha A, Scherer MN, Moser C, Karrasch T, Girlich C, Eder F, et al. A case study to living donor parathyroid allotransplantation for therapy-refractory postsurgical persistent hypoparathyroidism in a nontransplant recipient - three year results. BMC Surg. 2016;3;16(1):51.
  • 4. Bohrer T, Stelter K, Stelter IS, Dietrich C, Sitter H, Hasse C. Microencapsulation of allogeneic parathyroid tissue succeeds by delaying immunization of the host, not by protecting the graft against the host’s activated immune system. Transplantationsmedizin. 2008;18:36–42.
  • 5. Khryshchanovich V, Ghoussein Y. A case study to allotransplantation of macroencapsulated parathyroid cells as a treatment of severe postsurgical hypoparathyroidism. Ann Saudi Med. 2016;36:143‐147.
  • 6. Nawrot I, Wozniewicz B, Tolloczko T, Sawicki A, Gorski A, Chudzinski W, et al. Allotransplantation of cultured parathyroid progenitor cells without immunosuppression:clinical results. Transplantation. 2007;83:734‐740.
  • 7. McHenry CR, Stenger DB, Calandro NK. The effect of cryopreservation on parathyroid cell viability and function. Am J Surg. 1997;174:481.
  • 8. Wells SA JR, Gunnells JC, Shelburne JD, Schneider AB, Sherwood LM. Transplantation of parathyroid glands in man: clinical indications and results. Surgery. 1975; 78:34–44.
  • 9. Groth C, Hammond WS, Iwatsuki S, Popovtzer M, Cascardo S, Halgrimson C, et al. Survival of a homologous parathyroid implant in an immunosuppressed patient. Lancet. 1973; 19:1082–5.
  • 10. Kayaalp OS.Rasyonel Tedavi Yönünden Tibbi Farmakoloji. 11. Baskı. Hacettepe:Taş Yayınevi; 2005.
  • 11. Guerrero MA, Evans DB, Lee JE, Bao R, Bereket A, Gantela S, et al. Viability of cryopreserved parathyroid tissue: when is continued storage versus disposal indicated. World J Surg. 2008; 32:836-839.
  • 12. Aysan E, Kilic U, Gok O, Altug B, Ercan C, Kesgin Toka C, et al. Parathyroid Allotransplant for Persistent Hypocalcaemia: A new technique involving short-term culture. Exp Clin Transplant. 2016; 14(2):238-41.
  • 13. Aysan E, Altug B, Ercan C, Kesgin Toka C, Idiz UO, Muslumanoglu M. Parathyroid allotransplant with a new technique: A prospective clinical trial. Exp Clin Transplant. 2016; 14(4):431-5.
  • 14. Cabane P, Gac P, Amat J, Pineda P, Rossi R, Caviedes R, et al. A case study to allotransplant of microencapsulated parathyroid tissue in severe postsurgical hypoparathyroidism. Transplant Proc. 2009; 41:3879‐3883.
  • 15. Agha A, Moser C, Karrasch T, Girlich C, Eder F, Jung E, et al. A case study to living-donor parathyroid allotransplantation for therapy-refractory postsurgical persistent hypoparathyroidism in a nontransplant recipient – three year results. BMC surgery. 2016; 3;16(1):51.
  • 16. Cabane P. Parathyroid transplant: from autotransplant to stem cells differentiation. Int J Endocrinol Meta. 2016; 3(3):00051.
  • 17. Chapelle T, Meuris K, Roeyen G, De Greef K, Van Beeumen G, Bosmans JL, et al. a case study to simultaneous kidney-parathyroid allotransplantation from a single donor after 20 years of tetany. Transplant Proc. 2009; 41(2):599–600.
  • 18. Decker GA, Stark JH, Botha JR, Margolius LP, Decker G. Allotransplantation of parathyroid cells. Lancet. 1995; 14:124.
  • 19. Sollinger HW, Macke E, Cook K, Belzer FO. Allotransplantation of human parathyroid tissue without immunosuppression. Transplantation. 1983; 36, 599-609.
  • 20. Herrera M, Grant C, van Heerden JA, Fitzpatrick LA. Parathyroid autotransplantation. Arch Surg. 1992; 127:825-830.
  • 21. Hasse C, Zielke A, Klöck G, Schlosser A, Zimmermann U, Rothmund M. First successful xenotransplantation of microencapsulated human parathyroid tissue in experimental hypopara-thyroidism: Long-term function without immunosuppression. J Microencapsul. 1997; 14(5):617-26.
  • 22. Liu HG, Chen ZC, Zhang XH, Yang K. A case study to replantation with cryopreserved parathyroid for permanent hypoparathyroidism. Int J Clin Exp Med. 2015; 8(3): 4611-4619.
  • 23. Burlak C. Literature Update Xenotransplantation literature update, November– December 2014. Xenotransplantation 2015; 22: 80–83.
  • 24. Praud C, Vauchez K, Zongo P, Vilquin JT. Modelling human myoblasts survival upon xenotransplantation into immunodeficient mouse muscle. Exp Cell Res. 2018; 15;364(2):217-223.
  • 25. Nagy N, Marsiano N, Bruckner RS, Scharl M, Gutnick MJ, Yagel S, et al. Xenotransplantation of human intestine into mouse abdomen or subcutaneous tissue: Novel platforms for the study of the human enteric nervous system. J Neurogastroenterol Motil. 2018; 30(3).
  • 26. Abarrategi A, Mian SA, Passaro D, Rouault-Pierre K, Grey W et al. Modeling the human bone marrow niche in mice: From host bone marrow engraftment to bioengineering approaches. J Exp Med. 2018;16. pii: jem.20172139.
  • 27. Ayşan E, Düzköylü Y, Can İ, Büyükpınarbaşılı N. Xenotransplantation of human cryopreserved parathyroid tissue isolated from parathyroidadenomas to normocalcemic rabbits. Turk J Surg. 2017;1;33(2):91-95.
  • 28. Quinn R. Comparing rat’s to human’s age: How old is my rat in people years? Nutrition 2005;21:775‑7.
  • 29. Schmitt CP, HommeM, Schaefer F. Structural organization and biological relevance of oscillatory parathyroid hormone secretion. Pediatr Nephrol 2005; 20: 346–351.

Efficacy of xenotransplantation without immunosuppression in rats

Year 2019, Volume: 44 Issue: 3, 782 - 787, 30.09.2019
https://doi.org/10.17826/cumj.486224

Abstract

Purpose: .The aim of this study was to transplant hyperplastic cells obtained from human parathyroid tissues into the rats without immunsupression, and also evaluate the efficiency. 

Materials and Methods: Present study,hyperplasic parathyroid tissues were obtained and cells were isolated from a 62-year-old male.  Three male rats were used as recipients.Cells were obtained from human,transplanted into different anatomical locations of rats without immunosuppression. During the transplantation, cells were injected directly into the vena porta, vena cava and peripheral vena.Total parathormone (PTH) and serum calcium  levels were measured before and after transplantation in terms of follow-up of transplantation success.

Results: Total PTH levels of rats, before transplantation were determined 1.4-4.2 pg/mL and throughout 45 days values were detected 6.2-17.1 pg/mL. 

Conclusion: Peripheral circulation and vena porta transplantation was found more successful than vena cava.As a result, cell transplantation from the peripheral vein might be useful technique.In the future, new studies should be carried out by increasing the number of animals will be strengthen this research. 


References

  • 1. Shoback D. Clinical practice. Hypoparathyroidism. N Engl J Med. 2008;359:391–403.
  • 2. Leem TH, Volpi E, Eisele DW. Non-neural complications of thyroid and parathyroid surgery. In: Randolph GE.Surgery of the thyroid and parathyroid, 2nd edition Philadelphia: Elsevier Saunders, 2013;446-542.
  • 3. Agha A, Scherer MN, Moser C, Karrasch T, Girlich C, Eder F, et al. A case study to living donor parathyroid allotransplantation for therapy-refractory postsurgical persistent hypoparathyroidism in a nontransplant recipient - three year results. BMC Surg. 2016;3;16(1):51.
  • 4. Bohrer T, Stelter K, Stelter IS, Dietrich C, Sitter H, Hasse C. Microencapsulation of allogeneic parathyroid tissue succeeds by delaying immunization of the host, not by protecting the graft against the host’s activated immune system. Transplantationsmedizin. 2008;18:36–42.
  • 5. Khryshchanovich V, Ghoussein Y. A case study to allotransplantation of macroencapsulated parathyroid cells as a treatment of severe postsurgical hypoparathyroidism. Ann Saudi Med. 2016;36:143‐147.
  • 6. Nawrot I, Wozniewicz B, Tolloczko T, Sawicki A, Gorski A, Chudzinski W, et al. Allotransplantation of cultured parathyroid progenitor cells without immunosuppression:clinical results. Transplantation. 2007;83:734‐740.
  • 7. McHenry CR, Stenger DB, Calandro NK. The effect of cryopreservation on parathyroid cell viability and function. Am J Surg. 1997;174:481.
  • 8. Wells SA JR, Gunnells JC, Shelburne JD, Schneider AB, Sherwood LM. Transplantation of parathyroid glands in man: clinical indications and results. Surgery. 1975; 78:34–44.
  • 9. Groth C, Hammond WS, Iwatsuki S, Popovtzer M, Cascardo S, Halgrimson C, et al. Survival of a homologous parathyroid implant in an immunosuppressed patient. Lancet. 1973; 19:1082–5.
  • 10. Kayaalp OS.Rasyonel Tedavi Yönünden Tibbi Farmakoloji. 11. Baskı. Hacettepe:Taş Yayınevi; 2005.
  • 11. Guerrero MA, Evans DB, Lee JE, Bao R, Bereket A, Gantela S, et al. Viability of cryopreserved parathyroid tissue: when is continued storage versus disposal indicated. World J Surg. 2008; 32:836-839.
  • 12. Aysan E, Kilic U, Gok O, Altug B, Ercan C, Kesgin Toka C, et al. Parathyroid Allotransplant for Persistent Hypocalcaemia: A new technique involving short-term culture. Exp Clin Transplant. 2016; 14(2):238-41.
  • 13. Aysan E, Altug B, Ercan C, Kesgin Toka C, Idiz UO, Muslumanoglu M. Parathyroid allotransplant with a new technique: A prospective clinical trial. Exp Clin Transplant. 2016; 14(4):431-5.
  • 14. Cabane P, Gac P, Amat J, Pineda P, Rossi R, Caviedes R, et al. A case study to allotransplant of microencapsulated parathyroid tissue in severe postsurgical hypoparathyroidism. Transplant Proc. 2009; 41:3879‐3883.
  • 15. Agha A, Moser C, Karrasch T, Girlich C, Eder F, Jung E, et al. A case study to living-donor parathyroid allotransplantation for therapy-refractory postsurgical persistent hypoparathyroidism in a nontransplant recipient – three year results. BMC surgery. 2016; 3;16(1):51.
  • 16. Cabane P. Parathyroid transplant: from autotransplant to stem cells differentiation. Int J Endocrinol Meta. 2016; 3(3):00051.
  • 17. Chapelle T, Meuris K, Roeyen G, De Greef K, Van Beeumen G, Bosmans JL, et al. a case study to simultaneous kidney-parathyroid allotransplantation from a single donor after 20 years of tetany. Transplant Proc. 2009; 41(2):599–600.
  • 18. Decker GA, Stark JH, Botha JR, Margolius LP, Decker G. Allotransplantation of parathyroid cells. Lancet. 1995; 14:124.
  • 19. Sollinger HW, Macke E, Cook K, Belzer FO. Allotransplantation of human parathyroid tissue without immunosuppression. Transplantation. 1983; 36, 599-609.
  • 20. Herrera M, Grant C, van Heerden JA, Fitzpatrick LA. Parathyroid autotransplantation. Arch Surg. 1992; 127:825-830.
  • 21. Hasse C, Zielke A, Klöck G, Schlosser A, Zimmermann U, Rothmund M. First successful xenotransplantation of microencapsulated human parathyroid tissue in experimental hypopara-thyroidism: Long-term function without immunosuppression. J Microencapsul. 1997; 14(5):617-26.
  • 22. Liu HG, Chen ZC, Zhang XH, Yang K. A case study to replantation with cryopreserved parathyroid for permanent hypoparathyroidism. Int J Clin Exp Med. 2015; 8(3): 4611-4619.
  • 23. Burlak C. Literature Update Xenotransplantation literature update, November– December 2014. Xenotransplantation 2015; 22: 80–83.
  • 24. Praud C, Vauchez K, Zongo P, Vilquin JT. Modelling human myoblasts survival upon xenotransplantation into immunodeficient mouse muscle. Exp Cell Res. 2018; 15;364(2):217-223.
  • 25. Nagy N, Marsiano N, Bruckner RS, Scharl M, Gutnick MJ, Yagel S, et al. Xenotransplantation of human intestine into mouse abdomen or subcutaneous tissue: Novel platforms for the study of the human enteric nervous system. J Neurogastroenterol Motil. 2018; 30(3).
  • 26. Abarrategi A, Mian SA, Passaro D, Rouault-Pierre K, Grey W et al. Modeling the human bone marrow niche in mice: From host bone marrow engraftment to bioengineering approaches. J Exp Med. 2018;16. pii: jem.20172139.
  • 27. Ayşan E, Düzköylü Y, Can İ, Büyükpınarbaşılı N. Xenotransplantation of human cryopreserved parathyroid tissue isolated from parathyroidadenomas to normocalcemic rabbits. Turk J Surg. 2017;1;33(2):91-95.
  • 28. Quinn R. Comparing rat’s to human’s age: How old is my rat in people years? Nutrition 2005;21:775‑7.
  • 29. Schmitt CP, HommeM, Schaefer F. Structural organization and biological relevance of oscillatory parathyroid hormone secretion. Pediatr Nephrol 2005; 20: 346–351.
There are 29 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Research
Authors

Ebru Kanımdan 0000-0002-7123-4600

Emrah Yücesan 0000-0003-4512-8764

Beyza Göncü 0000-0001-6026-8218

Burcu Özdemir This is me 0000-0003-0288-0470

Oğuz İdiz 0000-0002-8462-7809

Yeliz Emine Ersoy This is me 0000-0002-5028-6436

Fahri Akbaş This is me 0000-0002-3837-250X

Erhan Ayşan This is me 0000-0002-9563-3761

Publication Date September 30, 2019
Acceptance Date December 31, 2018
Published in Issue Year 2019 Volume: 44 Issue: 3

Cite

MLA Kanımdan, Ebru et al. “Sıçanlarda immünsupresyonsuz Xenotransplantasyon uygulamasının etkililiği”. Cukurova Medical Journal, vol. 44, no. 3, 2019, pp. 782-7, doi:10.17826/cumj.486224.