Kadmiyum toksisitesi oluşturulan ratlarda arı sütünün farklı dozlarının oksidatif stres ve telomeraz enzimi üzerine etkileri

Year 2022, Volume: 47 Issue: 4, 1516 - 1522, 28.12.2022

Selcen Çakır

https://doi.org/10.17826/cumj.1139113

Cited By: 1

Abstract

Amaç: Kadmiyum (Cd), çevre kirlenmesi nedeni ile insan sağlığını ciddi oranda tehdit eden endüstride ve tarımda yaygın şekilde kullanılan oksidatif strese ve doku hasarlarına sebep olan toksik bir metaldir. Bu çalışma Cd ile oluşturulmuş doku hasarında arı sütünün (RJ) oksidatif statü ve telomeraz enzim aktivitesine etkisini araştırmayı amaçlamaktadır.
Gereç ve Yöntem: Deneysel tasarım her grupta 6 rat olacak şekilde; Kontrol grubu, Cd grubu, 250 mg/kg RJ, Cd + 250 mg/kg RJ, 400 mg/kg RJ, Cd + 400 mg/kg RJ grubu olacak şekilde 6 guruba ayrılarak oluşturuldu. Çalışmada kan serumundan total oksidan düzeyi, total antioksidan düzeyi kolorimetrik yöntemle ve ovaryum dokusundan telomeraz enzim aktivitesi ELISA yöntemiyle araştırıldı.
Bulgular: Cd oksidatif kapasitede artışa sebep oldu (23.80 ± 2.4) ve RJ uygulamaları ardından kontrol grubuna göre anlamlı azalma izlendi. RJ uygulaması ardından en iyi total antioksidan yanıtı 250 mg/kg RJ ve Cd + 250 mg/kg RJ gruplarında görüldü. Cd telomeraz enzim aktivitesini önemli düzeyde düşürmüştür. (0.90 ± 0.13) Cd uygulaması ardından tedavi amaçlı verilen RJ nin telomeraz düzeylerini yeniden kontrol seviyesine (1.40 ± 0.05) yükselttiği görüldü. Tedaviye en iyi cevap Cd + 250 mg/kg RJ grubunda izlendi (1.42 ± 0.05).
Sonuç: Cd un oksitatif strese sebep olduğu ve RJ uygulamaları ardından antioksidan kapasitede ve telomeraz enzim aktivitesinde görülen artışla RJ nin iyileştirici etkilerinin olabileceği sonucuna ulaşıldı.

Keywords

Arı Sütü, Kadmiyum, Telomeraz, Antioksidan, Ovaryum

Supporting Institution

yok

Project Number

yok

Effects of different doses of royal jelly on oxidative stress and telomerase enzyme in rats with Cadmium toxicity

Abstract

Purpose: Cadmium (Cd) is a toxic metal that seriously threatens human health due to environmental pollution, is widely used in industry and agriculture, and causes oxidative stress and tissue damage. This study aims to examine the effect of royal jelly (RJ) on oxidative status and telomerase enzyme activity in tissue damage induced by Cd.
Materials and Methods: The experimental design was made with 6 rats in each group. A total of 6 groups were created: control group, Cd group, 250 mg/kg RJ group, Cd + 250 mg/kg RJ group, 400 mg/kg RJ group, Cd + 400 mg/kg RJ group. In the study, total oxidant status and total antioxidant status in blood serum were investigated by colorimetric method, and telomerase enzyme activity in ovarian tissue was investigated by ELISA method.
Results: Cd caused an increase in oxidative capacity (23.80 ± 2.4) and a significant decrease was determined after RJ applications compared to the control group. After RJ application, the best total antioxidant response was observed in the 250 mg/kg RJ and Cd + 250 mg/kg RJ groups. Cd significantly reduced telomerase enzyme activity (0.90 ± 0.13). RJ administered for treatment after Cd application increased telomerase levels up to the control level (1.40 ± 0.05). The best treatment response was observed in the Cd + 250 mg/kg RJ group (1.42 ± 0.05).
Conclusion: Cd causes oxidative stress and that RJ may have curative effects by increasing the antioxidant capacity and telomerase enzyme activity RJ is a promising natural product and can contribute to recovery.

Keywords

Cadmium, Royal Jelly, Telomerase, Antioxidant, Ovary

Project Number

yok

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