Investigating the Effect of Heat Shock on Chromosomal Aberration of Vicia dasycarpa

Yıl 2015, Cilt: 36 Sayı: 3, 657 - 663, 13.05.2015

Sanaz Fareghi Varahram Rashidi Ahmad Razban Haghighi

Öz

Abstract. One of the wide applications of cytogenetic is evaluation of environmental factors on chromosomes. In this study, some seeds were boiled in bath water of 900 C for 3 minutes in order to study effects of heat shock on chromosomal aberration of hairy vetch. Then root-tip meristems of both kinds of seeds (i.e. normal seeds and seeds obtained under heat shock conditions) were studied from metaphase mitosis aspect. Normally plant is diploid with basic chromosome number x=7, (2n=2x=14). Cytogenetic studies of the seeds under heat shock conditions indicated mixoploidy state, in a way that diploid, aneuploid and tetraploid cells were observed in a root. Results of variance analysis and comparison of average normal treatments with heat shock treatments in diploid state indicated increased length of chromosomes and increased amount of genome. No aberration of chromosomal size was observed in tetraploid cells compared to normal state, just number of chromosomes increased twofold (2n=4X=28). Moreover study of some morphological traits indicated significant difference in the plant height of heat shock conditions compared to normal plants.

Anahtar Kelimeler

meristem, mixoploidy, aneuploid, metaphase, genome

Kaynakça

• Mozaffariyan,V. (2007), “Dictionary of Iranian Plants Names”, Amir Kabir Press, (in Persian).
• Karimi,H.( 2000), “Forage Plants Breeding and Cultivation”, University of Tehran Press, (in Persian).
• Gaffarzadeh Namazi, L., Badarzadeh, M. and Asghari Zakaria, R. (2008), “Karyotype of several vicia species from iran”, Asian J. of Plant Sciences, 7(4), pp. 417-420.
• Mazaheri Laghab, H., Aram, Y., Chaichi, M and Akbari, GH.( 2009), “An Introduction to Forage Plants” Aboo Ali Sina University Press, (in Persian).
• Feheimer, N .S. (1979), “Cytogenctic in Plant Production”, J. of Dairy Science, 62(5).
• Bajpai, A. and Singh, A.K. (2006), “Meiotic behavior of Caria Papaya: spontaneous chromosome instability and elimination in important cvs in north Indian Conditions”, Cytologia, 71, pp. 131-136.
• Saini, H. S. (1997), “Effect of water stress on male gametophyte development in plant sex”, Plant Reproduce, 10(6), pp. 67-73.
• Sun, K., Hunt, k . and Hauser, B.A. (2004), “Orule abortion in Arabidopsis triggered by stress”, Plant Physiol, 135, pp. 2358-2367.
• Vicini, L. F. and Carvalho, C. R. (2002), “Meiotic chromosomal variation. resulting from irradiation of pollen in maize”, J. Appl. Genet, 43, pp. 463-469
• Fairbanks, M.M., Hardy, G.E. and Mc Comb, J.A. (2002), “Mitosis and Miosis in plants are effected by the fungicide phosphate. Aust”, Plant Pathol, 31, pp. 281-289.
• Mir Mohammadi Meybodi, S.A.M and Tarkesh Isfahani,S. (2004), “ Racial and physiological aspects of cold stress and freezing in Forage Plants” Golbon Press, (in Persian).
• Haghani,S and Ebrahimi, M.(2009), “temperature stresses”, www.agrono millennium . com /pdf/tanesh damaee 2.pdf, (in Persian).
• Rui- Ju, L., Qiao - Ling, Q., Yue-fang,S., Run- tain, H., She- teng, K., Ling - bo, X .and Jian- hue, H. (2006), “Response of in vitro propagated plants of vicia faba to high temperature and alter violet stress”, J. of Nuclear Agri- Sci, 20(6), pp. 469-472.
• Kumar, G. and Tripathi, R. (2009), “Influence of heat stress on genome of grass pea”, J. of Environmental Biology, 30(3), pp. 405- 408 .
• Klindworth, D.L . and Williams, N.D. (2001), “characterization of a mitotic mutant of durum wheat”, Chromosome Research, 9,pp. 377-386.
• Farsi, M and Bagheri, A. (1999), “Principles of plant breeding”, Jahad Daneshgahi of Mashhad Press, (in Persian).
• Stebbins, G. L. (1971), “Chromosomal evolution in higher plants”, Edward Arnold Publisher LDT, London.