Determination of the Potential Use of Rhizosphere Bacteria in in vitro and in vivo Conditions for the Biological Control of Bacterial Seedling Blight Disease in Melon

Öz

In this study, the potential use of rhizosphere bacterial isolates in the biological control of Bacterial Seedling Blight Disease in melon caused by Acidovorax citrulli was investigated under in vitro and in vivo conditions. A total of 398 candidate antagonist bacteria, including 241 soil samples from the rhizosphere of various plants and 157 isolates from our laboratory collection, were screened for their in vitro antagonistic and plant growth promoting mechanisms (antibacterial activity, siderophore production capability, phosphorus solubilization ability, and indole acetic acid production levels). As a result of these tests, 8 bacterial isolates were selected for use in biological seed trials. In the first seed trial, the 8 applications suppressed disease severity by 0.48-39.45%. In the second seed trial with the two most successful isolates, Y90 isolate prevented the disease by 46.37% while Y28 isolate prevented the disease by 18.97%. Bacillus amyloliquefaciens subsp. plantarum Y90 and Providencia rettgeri Y28 were identified as successful antagonist bacterial isolates.

Anahtar Kelimeler

• Acidovorax citrulli
• Melon
• Antagonist
• Biological Control

Kavunda Bakteriyel Fide Yanıklığı Hastalığı’nın Biyolojik Mücadelesinde Rizosfer Bakterilerinin in vitro ve in vivo Koşullarda Kullanım Olanaklarının Belirlenmesi

Öz

Bu çalışmada, Acidovorax citrulli’nin neden olduğu kavunda Bakteriyel Fide Yanıklığı Hastalığı’nın biyolojik mücadelesinde rizosfer bakterilerinin kullanım olanakları in vitro ve in vivo koşullarda araştırılmıştır. Bu amaçla çeşitli bitkilerin rizosferinden alınan toprak örneklerinden 241 adet, laboratuvarımız koleksiyonundan 157 adet olmak üzere toplam 398 aday antagonist bakterinin in vitro antagonistik ve bitki gelişimini teşvik eden etki mekanizmaları (antibakteriyel etki, siderofor üretme yeteneği, fosforu çözme yeteneği ve indol asetik asit üretme miktarları) incelenmiştir. Bu testler sonucunda, 8 bakteriyel izolatın biyokontrol potansiyelleri in vivo koşullarda tohum denemeleri ile belirlenmiştir. Birinci tohum denemesinde, 8 uygulama hastalık şiddetini %0.48-39.45 oranında baskılamıştır. En başarılı iki izolat ile kurulan ikinci tohum denemesinde ise Y90 izolatı hastalığı %46.37 oranında engellerken Y28 izolatı hastalığı %18.97 oranında baskılamıştır. Başarılı antagonist bakteriyel izolatlar Bacillus amyloliquefaciens subsp. plantarum Y90 ve Providencia rettgeri Y28 olarak tanılanmıştır.

Anahtar Kelimeler

• Acidovorax citrulli
• Kavun
• Antagonist
• Biyolojik Mücadele

Kaynakça

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