Çukurova Bölgesi’nde Kayısı Ağaçlarında Solgunluk ve Gövde Çürüklüğü Etmeni Phytopythium vexans’ın Tanılanması

Yıl 2016, Cilt: 31 Sayı: 2, 59 - 68, 29.07.2016

Ali Endes Mukaddes Kayım

Öz

Adana, Mersin illerinde yetiştiriciliği yapılan kayısı ağaçlarında hastalığa neden olan Phytopythium türlerinin morfolojik ve kültürel özelliklerinin yanı sıra moleküler karakterizasyonlarını saptamak amacı ile 2010-2012 yılları arasında farklı bölgelerden Phytopythium spp. ile infekteli olduğundan şüphe edilen bitki örnekleri toplanmıştır. Bu çalışmada kullanılan izolatların tamamı ITS gen dizisine göre Phytopythium vexans (de Bary) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque 2014 olarak saptanmıştır. İzolatların koloni deseni ve radiyal gelişimi farklı ortamlarda çalışılmış ve mikroskobik yapıları belirlenmiştir. Bu çalışma sonucunda Phytopythium vexans’ın kayısı ağaçlarında solgunluk, kök ve kök boğazı çürüklüğü oluşturduğu, kayısı bahçelerinde ekonomik zarar oluşturabileceği saptanmıştır.

Anahtar Kelimeler

ITS bölgesi, Patojenisite, PCR, Phytopythium spp., Radyal Gelişim

Identification of Phytopythium vexans Causing Wilting and Trunk Rot of Apricot Trees in Çukurova Region

Öz

Suspicious plant materials with infected Phytopythium spp. were collected from apricot trees causing disease in different countrysides and locations of Adana and Mersin provinces for morphological and cultural features as well as molecular characterization of Phytopythium species during 2010-2012. All five isolates used throughout this study were identified as Phytopythium vexans (de Bary) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque 2014 based on ITS gen sequences. Colony patterns and radial growth study of isolates were performed on different media and subsequently their microscobic structures were identifıed. It was determined that Phytopythium vexans could cause wilting canopy, rotting of root and crown of apricot trees, which was the reason for economic damage in apricot orchards.

Anahtar Kelimeler

ITS Region, Pathogenicity, PCR, Phytopythium spp. Radial Growth

Kaynakça

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