Molecular fingerprinting of Botrytis cinerea population structure from different hosts

Öz

Botrytis cinerea (teleomorph: Botryotinia fuckeliana) causes gray mold disease on vegetable crops in greenhouses. Profound knowledge on pathogen diversity is necessary for efficiently disease management. In this study, forty-two B. cinerea isolates collected from 36 different greenhouses in Antalya province of Turkey were investigated. Twelve SRAP (sequence-related amplified polymorphism) and 18 ISSR (inter simple sequence repeat) primers producing high polymorphic fragments were used to genetic diversity of B. cinerea isolates infecting dill, basil, lettuce, bean, cucumber, tomato, pepper and eggplant. The unweighted pair-group method with arithmetic average analysis (UPGMA) was used to evaluate of combined ISSR and SRAP data showing a similarity range 0.15-0.90 among the isolates. Cophenetic correlation of the tree was high level (r=0.93). Interestingly, cluster analysis showed a divergent group consisting of lettuce isolates which were genetically different from the other isolates. On the other hand, transposable elements (Flipper and Boty) were detected among isolates from all the hosts. Isolates containing only the Fliper element were detected. The results showed that genetically characterized B. cinerea populations by a high level of genetic diversity were associated with genotype flow and the evolutionary potential of B. cinerea. In further studies, the newly tested molecular markers are useful and can be suggested for analyzing of genetic diversity and population structure of this pathogen on different hosts.

Anahtar Kelimeler

• ISSR,SRAP,ISSR,SRAP,Gray mold,Genetic diversity,Host differentiation,ISSR,SRAP,Transposable elements

Farklı konukçulardan elde edilen Botrytis cinerea populasyon yapısının moleküler tanılanması

Öz

Botrytis cinerea (teleomorph: Botryotinia fuckeliana) örtüaltı sebze yetiştiriciliğinde kurşuni küf hastalığı etmenidir. Patojende oluşan farklılıkların bilinmesi hastalıkla mücadelenin etkinliğini arttırmaktadır. Çalışmada, Türkiye’nin Antalya ilinde yer alan 36 farklı seradan 42 adet izolat kullanılmıştır. On iki SRAP (sequence-related amplified polymorphism) primer kombinasyonu ve 18 ISSR (inter simple sequence repeat) primeri dereotu, fesleğen, marul, fasulye, hıyar, domates, biber ve patlıcandan elde edilen B. cinerea izolatlarının genetik farklılıklarının belirlenmesinde oldukça yüksek polimorfizm sağlamışlardır. ISSR ve SRAP markırlardan elde edilen sonuçlar UPGMA (The unweighted pair-group method with arithmetic average analysis) analizine göre izolatlar arasında 0.15-0.90 oranında değişen benzerlik elde edilmiştir. Ayrıca, cophenetic correlation değeri r=0.93 ile oldukça yüksek bulunmuştur. Cluster analizi sonuçları değerlendirildiğinde marul izolatları diğer izolatlara göre oldukça uzak kümelenmiştir. Ayrıca, tüm izolatlar için tranpozabl elementler (Flipper ve Boty) araştırılmış ve sadece Flipper element tespit edilmiştir. Elde edilen genetik karakterizasyon sonuçlarına göre, B. cinerea populasyonunda oldukça yüksek seviyede genetik farklılıklar bulunmuştur. Bu durum, B. cinerea’nın evrimselleşme potensiyeli ve gen akışlarından kaynaklanabilir. Farklı konukçulardan elde edilen bu patojenin genetik farklılıklarının belirlenmesinde kullanılan moleküler markırlar, ileride yapılacak çalışmalara da ışık tutmaktadır.

Anahtar Kelimeler

• ISSR,SRAP,ISSR,SRAP,Kurşuni küf,Genetik farklılık,Konukçu farklılığı,ISSR,SRAP,Transpoazabl elementler

Kaynakça

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