Investigation of genetic diversity of different accessions of resistance against Phytophthora capsici L. using SRAP and SSR markers

Yıl 2019, Cilt: 36 Sayı: 2, 124 - 134, 10.12.2019

Münevver Göçmen , Kazım Abak

https://doi.org/10.16882/derim.2019.557877

Cited By: 2

Öz

Phytophthora capsici L. is one of the most important pathogens of pepper in the world.
Different genetic resources that resistant to P. capsici have been reported so far. Determination of the
phylogenetic relationships of resistant pepper genotypes is important for
transferring of resistance gene/genes to cultivated varieties. In this study,
12 pepper genotypes from different accessions of resistance and 4 susceptible
genotypes tested with P.
capsici were identified genetic relationship among genotypes using 144 SRAP
(Sequence Related Amplified Polymorphism) and 27 SSR (Simple Sequence Repeat)
primer combinations. 31 SRAP primers were polymorphic and yielded 254
polymorphic DNA fragments. Fifty SRAP primers were only monomorphic. However,
32 SRAP primers were not produced PCR products. Results showed that 16 pepper
genotypes were genetically separated from each other by SRAP primers. Twelve
SSR primers yielded total 36 DNA fragments, 33 of these were polymorphic (93%).
However 15 SSR primers only produced monomorphic DNA bands. Results showed that
Pepper genotypes PM-217 KM2-11, Perennial LS-279, PBC-178, Sera Demre, PBC 179
and KMAE-12 were not separated with SSR markers. When the SRAP and SSR
marker data were evaluated together, the results were more informative about the
genetic relationship of genotypes and clustering by origin. Findings indicated that the genotype CM 334 which is resistance resource
to P. capsici was genetically
different from PM 702, Perennial, PM-217, Taiwan's group called as PBC label
(1364, 1365, 178, 179 and 413) and KM211 selected from Kahramanmaraş pepper
populations.

Anahtar Kelimeler

Pepper, Phytophthora capsici L., SRAP, SSR, Genetic relationship

Phytophthora capsici'ye dayanıklı bazı biber genotiplerinin SRAP ve SSR belirteçlerle genetik farklılıklarının belirlenmesi

Öz

Dünyada biber yetiştiriciliğini sınırlayan en
önemli hastalıklardan birisi, kök boğazı yanıklığıdır. Bazı başka türlerde
olduğu gibi Capsicum annuum L. türüne
ait farklı genotiplerde de etmene karşı dayanıklılık kaynakları mevcuttur.
Dayanıklılık düzeyi farklı biber genotiplerinin genetik ilişkilerinin
belirlenmesi, dayanıklılık ile ilgili gen/genlerin kültür çeşitlerine
aktarılması açısından önemlidir. Bu çalışmada, P. capsici’ye karşı dayanıklılık düzeyi ve orijini farklı 12
dayanıklı ve 4 duyarlı biber genotipin filogenetik ilişkileri SRAP (Sequence
Related Amplified Polymorphism) ve SSR (Simple SequenceRepeat) moleküler
belirteçleriyle belirlenmiştir. Bunun için, 144 SRAP primer kombinasyonu ve 27
SSR primer çifti kullanılmıştır. Otuz iki SRAP primeri herhangi bir PCR ürünü
vermemiştir. Elli primer kombinasyonunda yalnızca monomorfik DNA bantı
oluşurken 31 primer kombinasyonu polimorfizm sağlamış toplam 254 DNA fragmenti
elde edilmiş, bunların, 99’u (%39) monomorfik, 155’i (%61) polimorfik DNAbandı
olarak değerlendirilmiştir. SRAP belirteçleri ile 16 biber genotipi birbirinden
genetik olarak ayrılmıştır. SSR primerinin 15’i monomorfik bant oluşturmasına
karşın 12 SSR primeri ile toplam 36 DNA bandı elde edilmiş bunların 33'ü (%93)
polimorfik olmuştur. SSR belirteç sistemi ile 16 biber genotipinin bazıları
(PM-217 KM2-11, Perennial LS-279, PBC-178, Sera Demre, PBC 179 KMAE-12)
birbirinden ayrılamamıştır. SRAP ve SSR belirteç verileri birlikte
değerlendirildiğinde, genotiplerin genetik ilişkisi, orijine göre kümelemesi
daha bilgi verici olmuştur. P. capsici’ye
karşı dayanıklılık genitörü olarak kullanılan CM 334'den, PM 702, Perennial,
PM-217, Tayvan'da temin edilen PBC grubu (1364, 1365, 178, 179 ve 413) ile
Kahramanmaraş biber populasyonundan selekte edilen KM211genotipleri genetik
olarak farklı gruplarda yer aldığı belirlenmiştir.

Anahtar Kelimeler

Biber, kök boğazı yanıklığı, SRAP, SSR, Genetik ilişki

Kaynakça

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