Analysis Of Inflammatory Cell Profile In Helicobacter Pylori Positive Gastrit Cases

Yıl 2016, Cilt: 30 Sayı: 1, 9 - 17, 24.06.2016

Ulaş Alabalık Hüseyin Büyükbayram Ayşe Nur Keleş Uğur Fırat

Öz

Introduction:
Being the most frequent and important factor in the etiology of chronic active
gastritis Helicobacter pylori can make up the basis for several diseases like
peptic ulcer, adenocarcinoma, and MALT lymphoma. It is important to determined
the profile of inflammatory cell in Helicobacter pylori gastritis for
lightening the pathogenesis of the diseases the Helicobacter pylori may cause.
In this study the inflammatory cell profile of the cases with Helicobacter
pylori gastritis was investigated. 

Methods: 100 cases
with Helicobacter pylori positive gastritis and 20 cases with Helicobacter
pylori negative gastritis were included in the study. In addition to these CD3,
CD4, CD5, CD8, CD10, CD20, CD68, CD79 α and CD138 immunohistochemical stains,
H&E and Giemsa stains were performed and detailed inflammatory cell analyse
was done.

Results: In
immunohistochemical investigation, considering the CD3, CD20, CD68 and CD79α
positivities, a significant difference was not determined between the groups.
It was established that while the number of inflammatory cells positively
reacted with CD10 and CD138 was increasing, the number of inflammatory cells
positively reacted with CD4, CD5 and CD8 was decreasing significantly with the
increasing amounts of Helicobacter pylori. Additionally CD138 positive plasma
cells were the most densely seen cell type in all the cases.

Discussion and Conclusion: It is
considered that in HP gastrits, the obvious increase of plasma cells and the
decrease of CD5 positive lymphocytes in the group which has the most HP
intensity might be playing a role in HP related stomach disease.

Anahtar Kelimeler

Helicobacter pylori, Inflammation, Immunohistochemistry

Helikobakter Pylori Pozitif Gastrit Vakalarında İnflamatuar Hücre Analizi

Öz

Amaç: Helikobakter pylori kronik aktif gastrit etiyolojisinde en
sık ve en önemli faktör olup, peptik ülser, adenokarsinoma ve MALT Lenfoma gibi
çeşitli hastalıklara da zemin hazırlayabilmektedir.Helikobakter pylori gastriti
olgularında inflamatuar hücre profilinin ortaya konması Helikobakter pylorinin
neden olabileceği hastalıkların patogenezine ışık tutabilmek için önemlidir. Bu
çalışmada Helikobakter pylori gastritli olgulardaki inflamatuar hücre profili
araştırıldı.

Yöntem ve Gereçler: Çalışmaya 100 adet Helikobakter pylori pozitif kronik
gastrit ve 20 adet Helikobakter pylori negatif gastrit vakası dahil edildi. Tüm
guruplarda H&E ve giemsa boyaması yanında ayrıca immünohistokimyasal olarak
CD3, CD4, CD5, CD8, CD10, CD20, CD68, CD79α ve CD138 boyamaları uygulandı ve
detaylı inflamatuar hücre analizi yapıldı.

Bulgular: İmmünohistokimyasal incelemede, Helikobacter pylori negatif
ve pozitif olgular arasında CD3, CD20, CD68 ve CD79α pozitiflikleri açısından
anlamlı bir farklılık saptanmadı. Helikobakter pylori miktarı arttıkça CD10 ile
ve CD138 ile pozitif reaksiyon veren inflamatuar hücre sayısı anlamlı derecede
artarken, CD4, CD5 ve CD8 pozitif olanların sayılarının ise anlamlı derecede
azaldığı tesbit edildi. Ayrıca CD138 pozitif plazma hücreleri tüm olgularda en
yüksek yoğunluktaki hücre tipiydi.

Tartışma ve Sonuç: HP
gastritlerinde HP’nin en yoğun olduğu grupta plazma hücrelerinin belirgin
biçimde artması ve CD5 pozitif lenfositlerin azalmasının HP ilişkili mide
hastalıklarının patogenezinde rol oynuyor olabileceği düşünülmüştür.

Anahtar Kelimeler

Helikobakter pylori, İnflamasyon, İmmünohistokimya

Kaynakça

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