CLOSE LOOK IN EMT, MAPK AND INFLAMMATION: WHAT DIFFERS BETWEEN MOUSE EMBRYONIC STEM, SOMATIC AND CANCER CELLS?

Yıl 2018, Cilt: 32 Sayı: 3, 201 - 212, 15.12.2018

Fatih Oltulu Berrin Özdil Çevik Gürel Eda Açıkgöz Duygu Çalık Kocatürk Yasemin Adalı Ayşegül Uysal Altuğ Yavaşoğlu Gülperi Öktem Hüseyin Aktuğ

https://doi.org/10.5505/deutfd.2018.49469

Öz

Objective: Life is a continuous cycle of the cells, composed of beginning, surviving and death. Cells keep dividing, differentiate and turn into somatic cells. The somatic cells at some point can give way to cancer cells and share characteristics of stem cells. The processes during these periods are important for identifying the tendency to abnormality and transformation to the cancer cells in somatic cells. To control emergence and progress of the cancer, mechanisms which take part in the survival become significant, especially EMT and inflammation. EMT occurs in both embryonic developmental stages and cancer progression and molecular basis of this process can be the therapeutic target for cure. Material and Method: For cancer representation mouse squamous lung cancer cells (SqLCCs), for somatic origin mouse skin fibroblasts (MSFs) and for embryonic stem cell mouse embryonic stem cells (mESCs) were used for comparison of three signaling pathways (EMT, MAPK and inflammation) at the gene expression level. Immunofluorescence staining protein levels of ERK 1/2, Vimentin and Twist were compared. Results: ERK1/2 protein expression similar in MSFs and SqLCCs while mESCs expression wasthe lowest. Besides, Twist and Vimentin expression statistically different in three. According to gene expression profiling of the EMT and inflammation supported by the MAPK signaling pathway are a far cry from each other at prominent genes especially Sparc, Vimentin, Mapksp1 and Il24.

Conclusion:
Three different cell linages showed different pattern both in gene and protein
expression. Therefore, these molecules can be the potential driving force for
therapeutic target

Anahtar Kelimeler

Cancer, epithelial- mesenchymal transition, inflammation, MAPK, embryonic stem cell

EMG, MAPK ve inflamasyona yakından bakış: Fare embriyonik kök hücre, somatik ve kanser hücrelerinde ne farklıdır?

Öz

Amaç: Yaşam; başlangıç, hayatta kalma ve
ölümü içeren hücrelerin devamlı olan döngüsünden oluşmaktadır. Hücreler devamlı
olarak bölünür, farklılaşır ve somatik hücreleri oluştururlar. Somatik hücreler
de belli bir noktada kanser hücrelerini oluşturup kök hücreler ile benzer
karakteristikleri paylaşabilmektedirler. Bu dönemlerdeki süreçler, somatik
hücrelerde anormalliğe eğilimi ve kanser hücrelerine dönüşümü tanımlamak için
önemlidir. Kanserin ortaya çıkışını ve ilerleyişini kontrol etmek için, hayatta
kalma mekanizmaları özellikle de epitelyal-mezenkimal geçiş (EMG) ve iltihaplanma
önemlidir. EMG, hem embriyonik gelişim aşamalarında, hem de kanser
ilerlemesinde ortaya çıkar ve bu süreç özellikle moleküler temelli tedavi için
terapötik hedef olabilir.

Gereç ve Yöntem: EMG, MAP-Kinaz ve
inflamasyon yolaklarının gen ekspresyon seviyesinde karşılaştırılmasında kanser
hücreleri temsili için fare skuamöz akciğer kanseri hücreleri (SqLCCs), somatik
kökenli hücre örneği olarak fare derisi fibroblastları (MSF'ler) ve embriyonik
kök hücre olarak da fare embriyonik kök hücreleri (mEKH'ler) kullanılmıştır.
ERK 1/2, Vimentin ve Twist'in immünofloresan boyama protein düzeyleri aynı
hücrelerde karşılaştırılarak incelenmiştir.

Bulgular: MSF'lerde ve
SqLCC'lerde ERK1/2 protein ekspresyonu benzer iken mEKH’lerde ekspresyonu en
düşük bulunmuştur. Ayrıca, Twist ve Vimentin ifadesi istatistiksel olarak üç
hücre hattında farklı çıkmıştır. EMG'nin gen ekspresyon profiline ve MAPK
sinyal yolağının desteklediği inflamasyona göre, özellikle Sparc, Vimentin,
Mapksp1 ve Il24 gibi belirgin genlerde ekspresyon profilleri birbirinden çok
farklı bulunmuştur.

Sonuç: Üç farklı hücre hattı hem gen hem
de protein ekspresyonunda farklı özellikler göstermiştir. Bu nedenle, bu
moleküller terapötik hedef belirlenmesinde potansiyel itici güç olabilir.

Anahtar Kelimeler

Kanser, Epitelyal mezenkimal geçiş, inflamasyon, MAPK, Embriyonik kök hücre

Kaynakça

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