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Evaluation of Helicobacter pylori antigen positivity in stool samples of patients with dyspeptic complaints in a tertiary care hospital

Yıl 2013, Cilt: 40 Sayı: 4, 574 - 578, 01.12.2013
https://doi.org/10.5798/diclemedj.0921.2013.04.0335

Öz

Objective: Helicobacter pylori is a microorganism associated with gastritis, peptic ulcer disease and gastric cancer. We aimed to figure out the positivity rate in stool samples of outpatients with dyspeptic complaints visiting gastroenterology department and to evaluate its relation with age, gender and seasonal changes. Methods: Between January 01, 2012 and December 31, 2012, stool samples of 330 adult outpatients admitted to gastroenterology department are investigated with an immunochromatographic test kit using monoclonal antibodies for detection of H. pylori antigen. Results: Among 330 patients\' stool samples tested, 67 (20.3%) were positive. 18.6% of men and 22.2% of women were detected as positive. According to age groups, 17.1% patients were positive for 15-35 age groups, 27.1% patients were positive for 36-55 age groups and 18.2% patients were positive for above 56. Seasonal difference of H. pylori antigen positivity in stool samples was statistically significant (p=0.001). Highest positivity rate 29.7% was detected for winter months (December-January-February). According to logistic regression analysis, winter is found as a risk factor with statistically significant 2.295 times greater risk [p=0001, Exp (B) = 2.925, 95.0% C.I. for EXP (B) = 1.668-5.129]. Conclusion: H. pylori antigen positivity rate of our study is lower than other previously conducted studies in Turkey. But, positivity rates are higher among women compared to men, concordant with other studies. Even more, detection of high positivity rates in winter shows primary infection and/or relapse can be affected by seasonal changes.

Kaynakça

  • Hestvik E, Tylleskar T, Kaddu-Mulindwa DH, et al. Heli- cobacter pylori in apparently healthy children aged 0-12 years in urban Kampala, Uganda: a community-based cross sectional survey. BMC Gastroenterology 2010;10:62-66.
  • Göral V, Özdal B, Kaplan A, et al. Diyarbakır ilinde Helico- bacter pylori antikor prevalansı. Akademik Gastroenterol Derg 2006;5:47-50.
  • Açık Y, Gülbayrak C, Dönder E, Yalnız M. Fırat Tıp merke- zine dispeptik yakınmalarla başvuran hastalarda Helicoba- cter pylori sıklığı ve etkileyen faktörler. J Exp Clin Med 2003;20:82-88.
  • Tünger Ö. Helicobacter pylori infeksiyonları. İnfeksiyon Dergisi 2008;22:107-115.
  • Özdemir M, Baykan M. Dispeptik hastalarda H. pylori infek- siyonu tanısında H. pylori gaita antijeninin tanı değerinin incelenmesi. Genel Tıp Derg 2005;15:65-70.
  • Sanders MK, Peura DA. Helicobacter pylori-associated dis- eases. Curr Gastroenterol Rep 2002;4:448-454.
  • Özkan TB. Çocuklarda H. pylori enfeksiyonunda seroloji, tanı ve tedavi. Uludağ Üni Tıp Fak Derg 2007;33:81-85.
  • Yücel T, Aygin D, Şen S, Yücel O. The prevalence of Helico- bacter pylori and related factors among university students in Turkey. Jpn J Infect Dis 2008;61:179-183.
  • Bulut M, Armağan E, Kıyıcı M, et al. Acil servise epigastrik ağrı yakınmasıyla başvuran hastalarda Helicobacter pylori sıklığı ve tanıda kalitatif serum IgG testinin yeri. Uludağ Üni Tıp Fak Derg 2004;30:7-10.
  • Da Silva KJM, Villares CA, Monteiro MS, et al. Valida- tion of a rapid stool antigen test for diagnosis of Helico- bacter pylori infection. Rev Inst Med Trop Sao Paulo 2010;52:125-128.
  • Demiray E, Yılmaz Ö, Şarkış C, et al. Comparison of inva- sive methods and two different stool antigen tests for diag- nosis of H. pylori infection in patients with gastric bleed- ing. World J Gastroenterol 2006;12:4206-4210.
  • Koneman EW, Allen SD, Janda WM, et al. Color Atlas and Textbook of Diagnostic Microbiology. 6th ed. Philadelphia: Lippincott-Raven Publishers, 2006:403-408.
  • Malfertheiner P, Megraud F, O’Morain C, et al. The Europe- an Helicobacter Study Group. Current concepts in the man- agement of Helicobacter pylori infection: the Maastricht III Consensus report. Gut 2007;56:772-781.
  • Ataseven H, Demir A, Keçeci M. Peptik ülsere bağlı üst gastrointestinal kanamalı olgularda Helicobacter pylo- ri eradikasyonunun fekal antijen testi ile tespiti. Fırat Tıp Derg 2004;18:199-204.
  • Prell C, Osterrieder S, Lottspeich C, et al. Improved per- formance of a rapid office-based stool test for detection of Helicobacter pylori in children before and after therapy. J Clin Microbiol 2009;47:3980-3984.
  • Demir T, Turan M, Tekin A. Kırşehir bölgesindeki dispeptik hastalarda Helicobacter pylori antijen prevalansı. Dicle Tıp Derg 2011;38(1):44-48.
  • Muhsen KH, Athamna A, Athamna M, Spungin-Bialik A, Cohen D. Prevalence and risk factors of Helicobacter py- lori infection among healthy 3- to 5-year old Israeli Arab children. Epidemiol Infect 2006;134:990-996.
  • Alim A, Ataş AD, Güneş T, et al. Sivas ili merkezinde semp- tomatik ve asemptomatik yetişkin bireylerde Helicobacter pylori seroprevalansı. CÜ Tıp Fak Derg 2004;26:75-80.
  • Kalem F, Özdemir M, Baysal B. Dispeptik Yakınmaları Olan Hastalarda Helicobacter pylori Varlığının Farklı Yön- temlerle Araştırılması. Mikrobiyol Bul 2010;44:29-34.
  • Replogle ML, Glaser SL, Hiatt RA, Parsonnet J. Biolog- ic sex as a risk factor for Helicobacter pylori infection in healthy young adults. Am J Epidemiol 1995;142:856-858.
  • Çıkman A, Parlak M, Güdücüoğlu H, Berktaş M. Van yöre- sinde Helicobacter pylori prevalansı, yaş ve cinsiyete göre dağılımı. Ankem Derg 2012;26:30-34.
  • Elwyn G, Taubert M, Davies S, et al. Which test is the best for Helicobacter pylori? A cost-effectiveness model using decision analysis. Br J General Practice 2007;57:401-403.
  • Dominguez-Bello MG, Beker B, Guelrud M, et al. Short report: socioeconomic and seasonal variations of Helico- bacter pylori infection in patients in Venezuela. Am J Trop Med Hyg 2002;66:49-51.
  • Moshkowitz M, Konikoff FM, Arber N, et al. Seasonal variation in the frequency of Helicobacter pylori infection: a possible cause of the seasonal occurrence of peptic ulcer disease. Am J Gastroenterol 1994;89:731-733.
  • Savarino V, Mela GS, Zentilin P, et al. Are duodenal ul- cer seasonal fluctuations paralleled by seasonal changes in 24-hour gastric acidity and Helicobacter pylori infection? J Clin Gastroenterol 1996;22:178-181.
  • Raschka C, Schorr W, Koch HJ. Is there seasonal periodic- ity in the prevalence of Helicobacter pylori? Chronobiol Int 1999;16:811-819.
  • Bhuiyan TR, Qadri F, Saha A, Svennerholm AM. Infection by Helicobacter pylori in Bangladeshi children from birth to two years: relation to blood group, nutritional status, and seasonality. Pediatr Infect Dis J 2009;28:79-85.
  • Wu IC, Wang SW, Yang YC, et al. Comparison of a new office based stool immunoassay and 13C-UBT in the diag- nosis of current Helicobacter pylori infection. J Lab Clin Med 2006;147:145-149.
  • Zambon CF, Basso D, Navaglia F, et al. Non-invasive di- agnosis of Helicobacter pylori infection: simplified 13C- urea breath test, stool antigen testing, or DNA PCR in hu- man feces in a clinical laboratory setting. Clin Biochem 2004;37:261-267.
  • Gisbert JP, Pajares JM. Stool antigen test for the diagnosis of Helicobacter pylori infection: A systematic review. Heli- cobacter 2004;9:347-368.
  • Gisbert JP, de la Morena F, Abraira V. Accuracy of mono- clonal stool antigen test for the diagnosis of H. pylori infec- tion: A systematic review and meta-analysis. Am J Gastro- enterol 2006;101:1921-1930.
  • Raguza D, Machado RS, Ogata SK, et al. Validation of a monoclonal stool antigen test for diagnosing Helicobacter pylori infection in young children. J Pediatr Gastroenterol Nut 2010;50:400-403.
  • Kolho KL, Klemola T, Koivusalo A, Rautelin H. Stool an- tigen tests for the detection of Helicobacter pylori in chil- dren. Diagn Microbiol Infect Dis 2006;55:269-273.

Evaluation of Helicobacter pylori antigen positivity in stool samples of patients with dyspeptic complaints in a tertiary care hospital

Yıl 2013, Cilt: 40 Sayı: 4, 574 - 578, 01.12.2013
https://doi.org/10.5798/diclemedj.0921.2013.04.0335

Öz

Amaç: Bu çalışmada; dispeptik yakınmalar ile hastanemiz gastroenteroloji polikliniğine ayaktan başvuran hastaların dışkı örneklerinde H. pylori antijen pozitifliğinin ve yaş, cinsiyet, mevsimsel değişiklikler ile olan ilişkisinin araştırılması amaçlanmıştır. Yöntemler: Çalışmamızda; 01 Ocak 2012-31 Aralık 2012 tarihleri arasındaki bir yıllık süre içerisinde gastroenteroloji polikliniğine ayaktan başvuran 330 erişkin hastanın dışkı örneklerinde monoklonal antikorların kullanıldığı immünokromatografik test ile H. pylori antijeni araştırıldı. Bulgular: Test edilen toplam 330 dışkı örneğinden 67\'si (%20,3) pozitif bulundu. Bu oran erkek hastalarda %18,6, kadın hastalarda ise %22,2 olarak tespit edildi. Yaş grubuna göre ise 15-35 yaş grubunda %17,1, 36-55 yaş grubunda %27,1, 56 yaş ve üzerindeki hastalarda ise %18,2 olarak saptandı. H. pylori antijen pozitifliğinin mevsimlere göre dağılımında istatistiksel olarak anlamlı bir farklılık tespit edildi (p=0,001). Buna göre en yüksek antijen pozitiflik oranı %29,7 ile kış mevsiminde (Aralık-Ocak-Şubat) bulundu. Yapılan lojistik regresyon analizinde kış aylarının H. pylori antijen pozitifliği yönünden 2,925 kat artırıcı bir risk faktörü olduğu tespit edildi (p=0,001). Sonuç: Çalışmamızda saptanan dışkı örneklerindeki H. pylori antijen pozitifliği, ülkemizde önceki yıllarda yapılmış çalışmalara göre daha düşük bulunmakla birlikte diğer çalışmalarla uyumlu olacak şekilde kadınlarda erkeklere göre daha yüksek düzeydedir. Ayrıca çalışmamızda kış aylarında diğer aylara göre daha yüksek pozitifliğin saptanması, primer enfeksiyon gelişimi, nüks veya bulaşın mevsimsel değişiklik gösterebileceğini ortaya koymaktadır.

Kaynakça

  • Hestvik E, Tylleskar T, Kaddu-Mulindwa DH, et al. Heli- cobacter pylori in apparently healthy children aged 0-12 years in urban Kampala, Uganda: a community-based cross sectional survey. BMC Gastroenterology 2010;10:62-66.
  • Göral V, Özdal B, Kaplan A, et al. Diyarbakır ilinde Helico- bacter pylori antikor prevalansı. Akademik Gastroenterol Derg 2006;5:47-50.
  • Açık Y, Gülbayrak C, Dönder E, Yalnız M. Fırat Tıp merke- zine dispeptik yakınmalarla başvuran hastalarda Helicoba- cter pylori sıklığı ve etkileyen faktörler. J Exp Clin Med 2003;20:82-88.
  • Tünger Ö. Helicobacter pylori infeksiyonları. İnfeksiyon Dergisi 2008;22:107-115.
  • Özdemir M, Baykan M. Dispeptik hastalarda H. pylori infek- siyonu tanısında H. pylori gaita antijeninin tanı değerinin incelenmesi. Genel Tıp Derg 2005;15:65-70.
  • Sanders MK, Peura DA. Helicobacter pylori-associated dis- eases. Curr Gastroenterol Rep 2002;4:448-454.
  • Özkan TB. Çocuklarda H. pylori enfeksiyonunda seroloji, tanı ve tedavi. Uludağ Üni Tıp Fak Derg 2007;33:81-85.
  • Yücel T, Aygin D, Şen S, Yücel O. The prevalence of Helico- bacter pylori and related factors among university students in Turkey. Jpn J Infect Dis 2008;61:179-183.
  • Bulut M, Armağan E, Kıyıcı M, et al. Acil servise epigastrik ağrı yakınmasıyla başvuran hastalarda Helicobacter pylori sıklığı ve tanıda kalitatif serum IgG testinin yeri. Uludağ Üni Tıp Fak Derg 2004;30:7-10.
  • Da Silva KJM, Villares CA, Monteiro MS, et al. Valida- tion of a rapid stool antigen test for diagnosis of Helico- bacter pylori infection. Rev Inst Med Trop Sao Paulo 2010;52:125-128.
  • Demiray E, Yılmaz Ö, Şarkış C, et al. Comparison of inva- sive methods and two different stool antigen tests for diag- nosis of H. pylori infection in patients with gastric bleed- ing. World J Gastroenterol 2006;12:4206-4210.
  • Koneman EW, Allen SD, Janda WM, et al. Color Atlas and Textbook of Diagnostic Microbiology. 6th ed. Philadelphia: Lippincott-Raven Publishers, 2006:403-408.
  • Malfertheiner P, Megraud F, O’Morain C, et al. The Europe- an Helicobacter Study Group. Current concepts in the man- agement of Helicobacter pylori infection: the Maastricht III Consensus report. Gut 2007;56:772-781.
  • Ataseven H, Demir A, Keçeci M. Peptik ülsere bağlı üst gastrointestinal kanamalı olgularda Helicobacter pylo- ri eradikasyonunun fekal antijen testi ile tespiti. Fırat Tıp Derg 2004;18:199-204.
  • Prell C, Osterrieder S, Lottspeich C, et al. Improved per- formance of a rapid office-based stool test for detection of Helicobacter pylori in children before and after therapy. J Clin Microbiol 2009;47:3980-3984.
  • Demir T, Turan M, Tekin A. Kırşehir bölgesindeki dispeptik hastalarda Helicobacter pylori antijen prevalansı. Dicle Tıp Derg 2011;38(1):44-48.
  • Muhsen KH, Athamna A, Athamna M, Spungin-Bialik A, Cohen D. Prevalence and risk factors of Helicobacter py- lori infection among healthy 3- to 5-year old Israeli Arab children. Epidemiol Infect 2006;134:990-996.
  • Alim A, Ataş AD, Güneş T, et al. Sivas ili merkezinde semp- tomatik ve asemptomatik yetişkin bireylerde Helicobacter pylori seroprevalansı. CÜ Tıp Fak Derg 2004;26:75-80.
  • Kalem F, Özdemir M, Baysal B. Dispeptik Yakınmaları Olan Hastalarda Helicobacter pylori Varlığının Farklı Yön- temlerle Araştırılması. Mikrobiyol Bul 2010;44:29-34.
  • Replogle ML, Glaser SL, Hiatt RA, Parsonnet J. Biolog- ic sex as a risk factor for Helicobacter pylori infection in healthy young adults. Am J Epidemiol 1995;142:856-858.
  • Çıkman A, Parlak M, Güdücüoğlu H, Berktaş M. Van yöre- sinde Helicobacter pylori prevalansı, yaş ve cinsiyete göre dağılımı. Ankem Derg 2012;26:30-34.
  • Elwyn G, Taubert M, Davies S, et al. Which test is the best for Helicobacter pylori? A cost-effectiveness model using decision analysis. Br J General Practice 2007;57:401-403.
  • Dominguez-Bello MG, Beker B, Guelrud M, et al. Short report: socioeconomic and seasonal variations of Helico- bacter pylori infection in patients in Venezuela. Am J Trop Med Hyg 2002;66:49-51.
  • Moshkowitz M, Konikoff FM, Arber N, et al. Seasonal variation in the frequency of Helicobacter pylori infection: a possible cause of the seasonal occurrence of peptic ulcer disease. Am J Gastroenterol 1994;89:731-733.
  • Savarino V, Mela GS, Zentilin P, et al. Are duodenal ul- cer seasonal fluctuations paralleled by seasonal changes in 24-hour gastric acidity and Helicobacter pylori infection? J Clin Gastroenterol 1996;22:178-181.
  • Raschka C, Schorr W, Koch HJ. Is there seasonal periodic- ity in the prevalence of Helicobacter pylori? Chronobiol Int 1999;16:811-819.
  • Bhuiyan TR, Qadri F, Saha A, Svennerholm AM. Infection by Helicobacter pylori in Bangladeshi children from birth to two years: relation to blood group, nutritional status, and seasonality. Pediatr Infect Dis J 2009;28:79-85.
  • Wu IC, Wang SW, Yang YC, et al. Comparison of a new office based stool immunoassay and 13C-UBT in the diag- nosis of current Helicobacter pylori infection. J Lab Clin Med 2006;147:145-149.
  • Zambon CF, Basso D, Navaglia F, et al. Non-invasive di- agnosis of Helicobacter pylori infection: simplified 13C- urea breath test, stool antigen testing, or DNA PCR in hu- man feces in a clinical laboratory setting. Clin Biochem 2004;37:261-267.
  • Gisbert JP, Pajares JM. Stool antigen test for the diagnosis of Helicobacter pylori infection: A systematic review. Heli- cobacter 2004;9:347-368.
  • Gisbert JP, de la Morena F, Abraira V. Accuracy of mono- clonal stool antigen test for the diagnosis of H. pylori infec- tion: A systematic review and meta-analysis. Am J Gastro- enterol 2006;101:1921-1930.
  • Raguza D, Machado RS, Ogata SK, et al. Validation of a monoclonal stool antigen test for diagnosing Helicobacter pylori infection in young children. J Pediatr Gastroenterol Nut 2010;50:400-403.
  • Kolho KL, Klemola T, Koivusalo A, Rautelin H. Stool an- tigen tests for the detection of Helicobacter pylori in chil- dren. Diagn Microbiol Infect Dis 2006;55:269-273.
Toplam 33 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Araştırma Yazıları
Yazarlar

Mehmet Burak Selek Bu kişi benim

Bayhan Bektöre Bu kişi benim

Orhan Baylan Bu kişi benim

Mustafa Özyurt Bu kişi benim

Yayımlanma Tarihi 1 Aralık 2013
Gönderilme Tarihi 2 Mart 2015
Yayımlandığı Sayı Yıl 2013 Cilt: 40 Sayı: 4

Kaynak Göster

APA Selek, M. B., Bektöre, B., Baylan, O., Özyurt, M. (2013). Evaluation of Helicobacter pylori antigen positivity in stool samples of patients with dyspeptic complaints in a tertiary care hospital. Dicle Tıp Dergisi, 40(4), 574-578. https://doi.org/10.5798/diclemedj.0921.2013.04.0335
AMA Selek MB, Bektöre B, Baylan O, Özyurt M. Evaluation of Helicobacter pylori antigen positivity in stool samples of patients with dyspeptic complaints in a tertiary care hospital. diclemedj. Aralık 2013;40(4):574-578. doi:10.5798/diclemedj.0921.2013.04.0335
Chicago Selek, Mehmet Burak, Bayhan Bektöre, Orhan Baylan, ve Mustafa Özyurt. “Evaluation of Helicobacter Pylori Antigen Positivity in Stool Samples of Patients With Dyspeptic Complaints in a Tertiary Care Hospital”. Dicle Tıp Dergisi 40, sy. 4 (Aralık 2013): 574-78. https://doi.org/10.5798/diclemedj.0921.2013.04.0335.
EndNote Selek MB, Bektöre B, Baylan O, Özyurt M (01 Aralık 2013) Evaluation of Helicobacter pylori antigen positivity in stool samples of patients with dyspeptic complaints in a tertiary care hospital. Dicle Tıp Dergisi 40 4 574–578.
IEEE M. B. Selek, B. Bektöre, O. Baylan, ve M. Özyurt, “Evaluation of Helicobacter pylori antigen positivity in stool samples of patients with dyspeptic complaints in a tertiary care hospital”, diclemedj, c. 40, sy. 4, ss. 574–578, 2013, doi: 10.5798/diclemedj.0921.2013.04.0335.
ISNAD Selek, Mehmet Burak vd. “Evaluation of Helicobacter Pylori Antigen Positivity in Stool Samples of Patients With Dyspeptic Complaints in a Tertiary Care Hospital”. Dicle Tıp Dergisi 40/4 (Aralık 2013), 574-578. https://doi.org/10.5798/diclemedj.0921.2013.04.0335.
JAMA Selek MB, Bektöre B, Baylan O, Özyurt M. Evaluation of Helicobacter pylori antigen positivity in stool samples of patients with dyspeptic complaints in a tertiary care hospital. diclemedj. 2013;40:574–578.
MLA Selek, Mehmet Burak vd. “Evaluation of Helicobacter Pylori Antigen Positivity in Stool Samples of Patients With Dyspeptic Complaints in a Tertiary Care Hospital”. Dicle Tıp Dergisi, c. 40, sy. 4, 2013, ss. 574-8, doi:10.5798/diclemedj.0921.2013.04.0335.
Vancouver Selek MB, Bektöre B, Baylan O, Özyurt M. Evaluation of Helicobacter pylori antigen positivity in stool samples of patients with dyspeptic complaints in a tertiary care hospital. diclemedj. 2013;40(4):574-8.