ADP, ATP, NAD, CoA, DNA VE RNA' NIN BiYOLOJiK KAYNAKLARDAN iZOLASYONU

Yıl 2000, Sayı: 001, 13 - 24, 15.01.2000

Vahdettin Bayazıt Sait Bulut

Öz

Bu caltsma; ADP, ATP, NAD, eoA, DNA ve RNA'ytfarkh biyolojik

kaynaklardan elde etmek icin yaptlmisttr. ATP erkek tavsanlartn

iskelet kaslartndan. ADP, ATP'den, NAD ve eoA mayadan, DNA

ve RNA ise erkek buzaginin timus bezi, dalak, karaciger, testis, bobrek,

akciger, kemik ilig] ve eritrositlerden elde edilmistir.

Bu calisma sonunda, 3 gr Ba2 ATP 4H20; 0.3 gr Ba3(ADP)z

4H20; 0.6 gr NAD; 15 gr eoA elde edilmistir. En cok DNA ve RNA

karacigerden, en az DNA bobrekten ve en az RNA ise akcigerden elde

edilmistir.

Anahtar Kelimeler

NAD, DNA ve RNA, ADP, ATP, CoA

ADP, ATP, NAD, eoA, DNA VE RNA' NIN BiYOLOJiK KAYNAKLARDAN iZOLASYONU

Öz

Bu caltsma; ADP, ATP, NAD, eoA, DNA ve RNA'ytfarkh biyolojik kaynaklardan elde etmek icin yaptlmisttr. ATP erkek tavsanlartn iskelet kaslartndan. ADP, ATP'den, NAD ve eoA mayadan, DNA ve RNA ise erkek buzaginin timus bezi, dalak, karaciger, testis, bobrek, akciger, kemik ilig] ve eritrositlerden elde edilmistir. Bu calisma sonunda, 3 gr Ba2 ATP 4H20; 0.3 gr Ba3(ADP)z
4H20; 0.6 gr NAD; 15 gr eoA elde edilmistir. En cok DNA ve RNA karacigerden, en az DNA bobrekten ve en az RNA ise akcigerden elde edilmistir.

Anahtar Kelimeler

ADP, ATP, NAD

Kaynakça

• Alberty, R.A., 1968, "Effect of pH and Metallon Concartration on the Equlibrion Hydrolysis of ATP to ADP", J. BioI. Chern. 243; 1337-1343.
• Bernhard,S., 1968, "The Structure and Function of Enzymes". W, A. Benjamin. Inc. New York.
• Blakley, R. L and Vitals, E., 1968, "Control of Nucleotide Biosynthesis." Ann. Rev. Biochem. 37: 201-224.
• Bray, H. G. and White, K., 1966, "Kinetics and Thermodynamics in Biochemistry, 2d ed. Academic Press Inc., Publishhers, New York.
• Burton, K., 1965, "Sequence Determination in Nucleic Acids" In P.N.
• Campbell and, G. D., Greville (eds), Essays in Biochemistry, VoU, Academic Press, New York, 57
• Cantoni, G. L. and Davies, D.(eds), 1966, "Procedures in nucleic acid research", Haper and Row, Publishers, Inc. New York.
• Colowick, S. P. and Kaplan, N. 0., 1957, "Methods in Enzymology" Vol. III. Academic Press, New York.
• Davidson, J. N., 1969, "The Biochemistry of the Nucleic Acids. Methuen. London, 6 the edition, A Popular and Useful Intraduction 350 p.
• Felsenfeld, G., and Miles H. T, 1967, "Physical and Chemical Properties of Nucleic Acids. Ann. Rev. Biochem .. 36: 407 - 448.
• Florkin, M. and Stotz, E. H. (eds), 1967. "Bioenerjetics", vol 22 of Comprehensive Biochemistry, American Elsevier, Publishhing Company, New York.
• George, P. and Rutman, R. J., 1960, "The high Energy Phosphate Bond Concept", Progr. Biophys. Chern., 10, I-53.
• Goodwin, T, W, J. I. Harris and B, S. Bentley (eds) 1964, "Structure and Activity of Enzymes Academic Press," Inc. New York.
• Gutfreund, H., 1965, "Intraduction to the Study of Enzymes," Wiley, New York.
• Hutchinson, D. W.. 1964, "Nucleotides and Coenzymes John Wiley and Sons," Inc. New York.
• Ingram, V. M., 1965,"The Biosynthesis of macromolecules",W. A., Benjamin, New York. A Primer.
• Josse, J., Kaiser, A. D. anel Korberg, A., 1961, "Enzymatic Synthesis of DNA, VIII. Frequence of Neaust Neighbor Base Sequence in DNA", J. BioI. Chern., 236; 864.
• Kaplan, N. O. and Kennedy, E. P. (eds), 1966, "Current Aspects of Biochemical Energetics", Academic Press Inc., New York.
• Kornberg, A., 1961, "Enzymatic Synthesis of DNA", Jonh Wilet and Sons, New York.
• Larsson, A and Reichard, P., 1967, "The Enzymatic Synthesis of Ribonucleotides, Progress in Nucleic Acid Research and Molecular Biology, 7: 303 - 347.
• Madison, J. T., 1968, "Primary Structure of RNA", Ann. Rev. Biochem, 37; 137- 148. Press Inc., Publishhers, New York.
• Slater, E. C. (ed.), 1966, "Flavins and Flavoproteins Elseiver Publishing Company," Amsterdam.