Application of PCR and PLS Tools for the Simultaneous Quantification of Praziquantel and Ivermectin Binary Mixtures in Veterinary Tablets

Abstract

Two chemometric calibration methods, principal component regression (PCR) and partial least squares regression (PLS) were proposed for the simultaneous spectrophotometric determination of praziquantel (PRA) and ivermectin (IVE) in a marketed veterinary formulation without using preliminary separation step. UV spectra of calibration set and samples including PRA and IVE were recorded in the spectral region of 225-315 nm. PCR and PLS algorithms were applied to absorbance data matrix and concentration set including PRA and IVE in the linear concentration range of 20.0-160.0 µg/mL for PRA and 2.0-44.0 µg/mL for IVE. The capability of the PCR and PLS methods were validated by analyzing validation samples. Assay results showed that both PCR and PLS approaches provided an opportunity for quantifying PRA and IVE in veterinary tablet formulation.

Keywords

• PCR and PLS calibrations
• Simultaneous quantification
• Praziquantel
• Ivermectin
• Veterinary tablet preparation

Veteriner Tabletlerinde Prazikuantel ve İvermektinin Eş Zamanlı Ölçümü için PCR ve PLS Yöntemlerinin Uygulanması

Abstract

Ticari bir veteriner formülasyonunda ön ayırma adımı kullanılmadan prazikuantel (PRA) ve ivermektinin (IVE) eş zamanlı spektrofotometrik tayini için iki kemometrik kalibrasyon yöntemi, temel bileşen regresyonu (PCR) ve kısmi en küçük kareler regresyonu (PLS) önerilmiştir. Kalibrasyon setinin UV spektrumları ve PRA ve IVE içeren numuneler 225-315 nm spektral bölgede kaydedildi. PRA için 20.0-160.0 µg/mL ve IVE için 2.0-44.0 µg/mL lineer konsantrasyon aralığında PRA ve IVE içeren absorbans veri matrisi ve konsantrasyon setine PCR ve PLS algoritmaları uygulandı. PCR ve PLS yöntemlerinin kapasitesi, doğrulama örnekleri analiz edilerek doğrulandı. Değerlendirme sonuçları, hem PCR hem de PLS yaklaşımlarının, veteriner tablet formülasyonunda PRA ve IVE'yi ölçmek için bir fırsat sağladığını gösterdi.

Keywords

• PCR ve PLS kalibrasyon
• Eşzamanlı miktarsal ölçüm
• Prazikuantel
• Ivermectin
• Veteriner tabletleri

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