Quantitative HPLC Analysis of Vitamin K3-Amprolium HCl-Methylparaben-Propylparaben in Oral Solutions

Abstract

HPLC ile Enjeksiyon Çözeltilerde Vitamin K3-Amprolyum HCl-Metilparaben-Propilparaben Miktar Tayini

Abstract

Bu çalışmada antiprotozoal etkili amprolyum HCl, kanın pıhtılaşmasını önleme açısından etkili vitamin K3, antimikrobiyal koruyucu etkili metilparaben ve propilparabeni bir arada içeren oral enjeksiyon çözeltisinin zaman açısından avantajı nedeniyle tek bir HPLC yöntemi ile miktar tayini ve validasyon çalışmaları yapılmıştır. Analizlerde 25°C'de İnertsil C8-3 4,6 x 250 mm, 5 µm kolon, 254nm’de dalga boyunda heptan-1-sülfonik asit sodyum tuzu, glasiyel asetik asit, trietilamin tampon çözeltisi ve asetonitril ve metanol kullanılmıştır. Seçicilik, sistem uygunluk, doğrusallık ve doğruluk parametreleri çalışılmış ve kabul edilebilir sonuçlar elde edilmiştir.

Keywords

• Vitamin K3,Amprolyum HCl,Metilparaben,Propilparaben,HPLC

References

1. [1] W. Song, M. Huang, W. Rumbeiha, H. Li, “Determination of Amprolium, Carbadox, Monensin, and Tylosin in Surface Water by Liquid Chromatography/Tandem Mass Spectrometry,” Rapid Commun Mass Spectrom, vol. 21, pp. 1944–1950, 2007.
2. [2] F. Belal, S.A.El-Razeq, M. Fouad, S. Zayed and F. Fouad, “Simultaneous Determination of Five Coccidiostates in Veterinary Powders, Feed Premixes, and Baby Food by Micellar Electrokinetic Chromatography: Application to Chicken Tissues and Liver,” Food Analytical Methods, vol. 11, pp. 3531-3541, 2018.
3. [3] E. Yarsan, S. Yazar, İ. Kutlu, H. Saltaş, “Amprolyumun Tavşanlarda İmmun Sistemin Bazı Parametreleri Üzerine Etkileri,” Türk Hij Den Biyol Derg, c. 60, No; 2, ss. 49-54, 2003.
4. [4] L. P. Joyner And C. C. Norton, “The Anticoccidial Effects Of Amprolium, Dinitolmide And Monensin Against Eimeria Maxima, E. brunetti and E. acervulina with Particular Reference to Oocyst Sporulation,” Parasitology, vol. 75, pp. 155-164, 1977.
5. [5] E. S. Namıduru, M. Tarakçıoğlu, “K Vitamini ve Osteoporoz,” Gaziantep Tıp Derg, vol. 17, no.1, pp. 1-7, 2011.
6. [6] K. N. Prasad, J. Edwards-Prasad, A. Sakamoto, “Vitamin K3 (Menadione) Inhibits the Growth of Mammalian Tumoe Cells in Culture,” Life Sci., vol. 29, no.13, pp. 1387-1392, 1981.
7. [7] E. O. Ngo, T. P. Sun, J. Y. Chang, C. C. Wang, K. H. Chi, A. L. Cheng, L. M. Nutter, “ Menadione-Induced DNA Damage in a human Tumor Cell Line,” Biochem Pharmacol, vol. 42, pp. 1961-1968, 1991.
8. [8] R. Ni, Y. Nishikawa, B. I. Carr, “Cell Growth Inhibition by a Novel Vitamin K is Associated with Induction of Protein Tyrosine Phosphorylation,” J. Biol. Chem., vol. 273, pp. 9906-9911, 1998.

9. [9] A. M. Daines, R. J. Payne, M. E. Humphries and A. D. Abell, “The Synthesis of Naturally Occurring Vitamin K and Vitamin K Analogues,” Current Organic Chemistry, vol. 7, pp. 1-15, 2003.
10. [10] S. Sakamoto, F. Taura, B. Pongkitwitoon, W. Putalun, R. Tsuchicashi, J. Kinjo, H. Tanaka, S. Morimoto, “Development of Sensitivity-Improved Fluorescence-Linked Immunosorbent Assay Using a fluorescent Single-Domain Antibody Against the Bioactive Naphthoquinone, Plumbagin,” Anal Bioanal Chem., vol. 396, pp. 2955-2963, 2010.
11. [11] V. M. Gohil, S. A. Sheth, R. Nilsson, A. P. Wojtovich, J. H. Lee, F. Perocchi, W. Chen, C. B. Clish, P. S. Brookes ve V. K. Mootha, “Nutrient-Sensitized Screening for Drugs That Shift Energy Metabolism from Mitochondrial Respiration to Glycolysis,” Nature Biotechnology, vol. 28, pp. 249-255, 2010.
12. [12] W. Hu, S. Tedesco, B. McDonagh, J. A. Barcena, C. Keane, D. Sheehan, “Selection of Thiol-and Disulfide-Containing Proteins of Escherichia Coli on Activated Thiol-Sepharose,” Anal. Biochem., vol. 398, no2, pp. 245-253, 2010.
13. [13] F. Kalyoncu, “Gıda Sanayinde Sıklıkla Kullanılan Antifungal Katkı Maddeleri,” New World Sciences Academy Natural and Applied Sciences, vol. 3, no. A0087, pp. 465-473, 2008.
14. [14] Haynes, W.M. (ed.), “CRC Handbook of Chemistry and Physics,” CRC Press LLC, Boca Raton, 95th Edition, pp. 3-470, 2014-2015.
15. [15] M. Ghanem, S. Abu-Lafi, R. Karaman, H. Hallak, “Validated HPLC Method to Simultaneously Determine Amprolium Hydrochloride, Sulfaquinoxaline Sodium and Vitamin K3 in A.S.K Powder on ZIC-HILIC Column,” Pharmaceutica Analytica Acta, vol. 168, no.3, pp. 2153-2435, 2012.
16. [16] B. Saad, Md. F. Bari, M. I. Saleh, K. Ahmad, M. K. M. Talib, “Simultaneous Determination of Preservatives (benzoic acid, sorbic acid, methylparaben and propylparaben) in Foodstuffs Using High-Performance Liquid Chromatography,” Journal of Chromatography A, vol. 1073, pp. 393-397, 2005.