Optimization of KI as X-Ray Computed Microtomography Contrast Agents for Murine and Chicken Epidermal Tissues Applications

Abstract

X-ray computed tomography (CT) vastly used in many different applications in different fields such as materials science, medical science, entomology, anatomy, marine sciences. Since the X-ray is highly penetrable, 3D image of almost any material can be achieved by CT. The high quality image of the materials, which compose in different types of atoms, can easily be achieved. However, obtain the high quality images of the materials which has similar types of atoms or relatively soft structure becomes a problem. Scientists investigating the soft tissues such as flesh, muscle, cartilage or animals in soft structure suffer from this problem. At this point, staining procedures, treating materials with contrast agents help the researcher to enhance the image quality. In this work, optimisation of KI based staining to obtain enhanced image quality in CT imaging of murine and chicken dermal tissues were studied. Results indicate that overstaining or staining the tissues in less concentration significantly affects the quality of the obtained CT images.

Keywords

• Computed Tomography
• Skin
• Phase Contrast Imaging
• Absorption Imaging
• Staining
• Soft Tissue

KI’nın Tavuk ve Kemirgen Epidermal Dokusunun Bilgisayarlı Tomografi Uygulamalarında Kontrast Ajanı Olarak Optimize Edilmesi

Abstract

Bilgisayarlı Tomografi (CT ya da BT) malzeme bilimi, tıp, entomoloji, deniz bilimleri anatomi gibi birçok alanda değişik uygulamalarda kullanılmaktadır. X-ışınlara yüksek derecede penetrasyon (maddelerin içerisinden geçebilme) özelliğine sahip olduğundan hemen hemen bütün malzemelerin 3 boyutlu görüntüsü BT ile elde edilebilmektedir. Birden fazla materyal bir araya gelerek oluşmuş kompozit malzemelerin yüksek kalitede görüntüsü kolaylıkla elde edilebilmektedir. Ancak aynı tipte atomlardan oluşan malzemelerin ya da sertlikleri birbirine çok yakın olan yumuşak malzemelerin yüksek kaliteede görüntülerinin elde edilmesi zordur. Kıkırdak, kas dokusu gibi dokularla yumuşak yapıdaki hayvanları inceleyen bilim insanları yüksek kalitede görüntü alamama gibi probleme sıkça rastlamaktadır. Bu sorunu aşmak için lekeleme prosedürü yani incelenecek malzemeyi kontrast ajanına maruz bırakmak araştırmacıların yüksek kalitede görüntü elde etmesine yardımcı olmaktadır. Bu çalışmada KI temelli kontrat ajanının tavuk ve fare derisinin BT uygulamasında görüntü kalitesinin arttırılması amacı ile optimize edilmesi incelenmiştir. Elde edilen sonuçlar lekeleme prosedüründe aşırı ya da az KI uygulanmasının görüntü kalitesini düşürdüğü göstermektedir.

Keywords

• Bilgisayarlı Tomografi
• Deri
• Faz Kontrast Görüntüleme
• Soğurma Temelli Görüntüleme
• Lekeleme
• Yumuşak Doku

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