Metal-Ion Assisted Imprinted Hydrogels For Recognition Of Lysozyme

Yıl 2021, , 545 - 555, 29.05.2021

Kemal Çetin

https://doi.org/10.29130/dubited.891731

Cited By: 1

Öz

Hydrogels exhibiting selectivity towards lysozyme were produced by metal-ion assisted-imprinting technology. A metal-chelate monomer N-vinyl-2-pyrrolidone is pre-assembled with the template molecule via assistance of Cu(II) ions and co-polymerized with 2-hydroxyethyl methacrylate. Lysozyme imprinted hydrogels were characterized by Fourier transform infrared spectroscopy, swelling tests, scanning electron microscopy. The conditions for the optimum adsorption capacity of the hydrogels towards lysozyme were found out by investigating the effects of initial concentration of lysozyme, medium pH, contact time and ionic strength. Maximum adsorption of lysozyme on poly(hydroxyethyl methacrylate-co-N-vinyl pyrrolidone) hydrogel was found to be 12.25 mg/g for 1.0 mg/mL initial concentration of lysozyme at 25.0°C with an optimal pH of 7.0. After ten adsorption-desorption cycles with the same hydrogel, the lysozyme adsorption capacity decreased by 13.80%.

Anahtar Kelimeler

Hydrogels, lysozyme, metal ion coordination, molecular imprinting

Lizozim Tanımada Metal İyon Destekli Baskılanmış Hidrojeller

Öz

Lizozime karşı seçicilik sergileyen hidrojeller, metal iyon aracılı baskılama teknolojisi ile üretildi. Metal şelat monomeri olarak N-vinil-2-pirolidon, Cu(II) iyonlarının yardımıyla kalıp molekül ile önceden kompleksleştrildi ve 2-hidroksietil metakrilat ile birlikte polimerleştirildi. Lizozim baskılanmış hidrojeller, Fourier dönüşümü kızılötesi spektroskopisi, şişme testleri, taramalı elektron mikroskobu ile karakterize edildi. Hidrojellerin lizozime karşı optimum adsorpsiyon kapasitesi için en uygun koşullar, lizozimin başlangıç derişiminin, ortam pH'sının, adsorpsiyon süresinin ve iyonik kuvvetin adsorpsiyon kapasitesine etkileri araştırılarak bulunmuştur. Lizozimin poli(hidroksietil metakrilat-ko-N-vinil pirolidon) hidrojel üzerinde maksimum adsorpsiyonu, 25.0°C'de 1.0 mg/mL başlangıç lizozim derişimi optimum pH değeri (7.0) için 12.25 mg/g olarak bulunmuştur. Aynı hidrojel ile on adsorpsiyon-desorpsiyon döngüsünden sonra, lizozim adsorpsiyon kapasitesi %13.80 azalmıştır.

Anahtar Kelimeler

Hidrojeller, lizozim, metal iyon koordinasyonu, moleküler baskılama

Kaynakça

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