Kilis Topraklarından İzole Edilen Bacillus B10 Suşunun Üreaz Üretiminin İstatiksel Optimizasyonu

Yıl 2021, Cilt: 9 Sayı: 5, 1782 - 1792, 31.10.2021

Hatice Aysun Mercimek Takcı , Pemra Bakırhan

Öz

Bu çalışmada Kilis ili toprak örneklerinden üreaz üretici Bacillus spp. suşları izole edilmiştir. B10 ve B11 olarak isimlendirilen suşlardan saflaştırılan intraselüler üreazın spesifik aktiviteleri 1.38±0.07 ve 1.22±0.02 µmol/min/mg’dır. B10 suşunun üreaz üretimini arttırabilmek için “Design Expert statistical software” (Design Expert 8.0.7)’in kullanıldığı Box Behnken deneme planı uygulanmıştır. Maksimum üreaz üretimi %5.00 üre, %3.00 sükroz ve %2.00 et özütü içeren besi ortamında gözlenmiş ve optimum koşullardaki en yüksek spesifik aktivite 1.498±0.05 µmol/min/mg olarak hesaplanmıştır.

Anahtar Kelimeler

Bacillus spp.,, Box Behnken, üreaz, Yüzey yanıt metodolojisi

Teşekkür

Çalışmamızda Kilis 7 Aralık Üniversitesi Lisansüstü Eğitim Enstitüsü tarafından onaylanan “Üreaz Üreticisi Bacillus spp. Mineralizasyonu ile CaCO3'ın Presipitasyonu ve Karakterizasyonu” konulu tezin verilerinden yararlanılmıştır. Bu sebeple Kilis 7 Aralık Üniversitesi’ne teşekkürlerimizi sunarız.

Statistical Optimization of Urease Production by Bacillus B10 Strain Isolated From Kilis Soils

Öz

In this study, urease producer Bacillus spp. strains were isolated from Kilis soil samples. The specific activities of intracellular urease purified from strains named as B10 and B11 were 1.38±0.07 and 1.22±0.02 µmol/min/mg. Box Behnken design using "Design Expert statistical software" (Design Expert 8.0.7) was applied to increase the urease production of B10 strain. Maximum urease production was observed in a medium containing 5.00% urea, 3.00% sucrose and 2.00% meat extract and the highest specific activity under optimum conditions was calculated as 1.498 ± 0.05 µmol/min/mg.

Anahtar Kelimeler

Bacillus spp., Box Behnken, urease, Surface Response Methodology

Kaynakça

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