The Use of Saliva Drying Tests for Detection of Oestrus in Holstayn Cattle

Öz

In the present study, it was investigated whether this method can be used in the estrus period, as the prevalence of woody or fern appearance, which is the result of easy-to-apply and low-cost saliva drying (crystallization) tests, changes in estrus and diestrus in holstein cattle. For this purpose, samples taken from holstay cattle, which were determined to be in estrus (n:14) and known to be in diestrus (n:11), were examined. The results were divided into 3 groups as "absent", "outlier" and "significant". It was determined that 71.4% of the cattle in estrus and 18.2% of the cattle in diestrus had a woody or fern appearance and the difference was significant. It was determined that 14.3% of the cattle in estrus and 63.6% of the cattle in diestrus had an outcrop or fern appearance and the difference was significant. It was determined that 14.3% of cattle in estrus and 18.2% of cattle in diestrus did not have woody or fern appearance, and the difference between the groups was insignificant.. As a result, according to the results of the saliva drying test, it was concluded that the estrus period in holstein cattle can be distinguished from the diestrus period.

Anahtar Kelimeler

• Artifical Insemination Time
• Fern
• Oestrus
• Saliva

Holstayn Sığırlarda Kızgınlık Tespitinde Tükürük Kurutma Testlerinin Kullanılması

Öz

Sunulan çalışmada uygulanması kolay ve maliyeti düşük olan tükürük kurutma (kristalizasyon) testleri sonucu oluşan ağaçlanma veya eğrelti otu görünümünün yaygınlığının holstayn ırkı sığırlarda östrusta ve diöstrusta değişip değişmediği dolaysıyla östrus döneminin taininde bu yöntemin kullanılıp kullanılamayacağı araştırıldı. Bu amaçla östrusta olduğu belirlenen (n:14) ve diöstrusta olduğu bilinen (n:11) holstayn sığırlardan alınan numuneler incelendi. Sonuçlar “yok”, “yüzlek” ve “belirgin” olarak 3 gruba ayrıldı. Östrustaki sığırların %71.4’ünde diöstrustaki sığıların % 18.2’ sinde ağaçlanma veya eğrelti otu görünümünün belirgin olduğu ve aradaki farkın anlamlı olduğu belirlenmiştir. Östrustaki sığırların % 14.3 ‘ ünde diöstrustaki sığırların % 63.6’sında ağaçlanma veya eğrelti otu görünümünün yüzlek olduğu ve aradaki farkın anlamlı olduğu belirlenmiştir. Östrustaki sığırların % 14.3’ünde diöstrustaki sığırların % 18.2’sinde ağaçlanma veya eğrelti otu görünümünün bulunmadığı ve gruplar arasındaki farkın önemsiz olduğu belirlenmiştir. Sonuç olarak tükürük kurutma testi sonuçlarına göre holstayn ırkı sığırlarda östrus döneminin diöstrus döneminden ayırt edilebileceği kanısına varılmıştır.

Anahtar Kelimeler

• Eğrelti otu
• Östrus
• Suni tohumlama zamanı
• Tükürük

Kaynakça

1. 1-Cirit Ü, Özmen MF, Ak K. (2017). Suni Tohumlamanın Başarısını Etkileyen Faktörler,Sahada Tohumlama Uygulamalarında Sıkça Yapılan Hatalar ve Çözüm Önerileri. Turkiye Klinikleri J Reprod Artif Insemin-Special Topics 3(1),30-43.
2. 2-Cirit Ü, Özmen MF, Nur Z. (2017). Suni Tohumlama Uygulamalarında Karşılaşılan Sorunlar, Çözüm Önerileri ve Sabit Zamanlı Tohumlama. Turkiye Klinikleri J Reprod Artif Insemin-Special Topics 3(1), 1-21.
3. 3-Zoldag L, Kecskemethyl S, Nagy P. (1993). Heat Progesterone Profiles Of Bitches With Ovulation Failure. Journal of Reproduction and Fertility. 1: 562-563.
4. 4-Johnston SD, Olson PN, Root MV. (1994). Clinical Approach To İnfertility İn Bitches. Seminars in Veterinary Medicine and Surgery (Small Animal). 9: 2-6.
5. 5- Papanicalou GN (1946). A General Survey Of The Vaginal Smear And İts Use İn Research And Diagnosis. Am J Obstet Gynecol. 51: 316-328.
6. 6- Garm O, Skjerven O. (1952). Investigation Of Cervikalslim For The Diagnosis Of Early Pregnancy And Endocrine Disorders Contingent Of Sexual Cycles With Livestock. Nordic. Vet. Medicin. 4: 1098- 1103.
7. 7- Noonan JJ, Schultze AB, Ellington EF. (1975). Changes İn Bovine Cervical And Vaginal Mucus During The Estrous Cycle And Early Pregnancy. J Anim Sci. 41: 1084-1089.
8. 8-Peterson DL. (1984). Nasal mucus ferning patterns (Doctoral dissertation, College of Nursing, University of Utah).

9. 9- Pardo-Carmona B, Moyano, MR, Fernandez-Palacios R, Perez-Marin CC. (2010). Saliva Crystallisation As A Means Of Determining Optimal Mating Time İn Bitches. Journal of Small Animal Practice. 51: 437-442.
10. 10- Golding TR, Brennan NA. (1989). The Basis Of Tear Ferning. Clinical and Experimental Optometry. 72: 102- 112.
11. 11- Zondek B, Rozin S. (1954). Cervical Mucus Arborization. Its Use İn The Determination Of Corpus Luteus Function. Obstet Gynecol. 3: 463-470.
12. 12- Mac Donald RR. (1969). Cyclic Changes İn Cervical Mucus. Journal of Obstetrics and Gynecology of the British Common wealth. 76: 1090-1099.
13. 13- Linford E. (1974). Cervical Mucus: An Agent Or A Barrier To Conception? Journal of Reproduction and Fertility. 37: 239-250.
14. 14- Roland M. (1971). Can The Fern Test Be Graded? Obstet Gynecol. 37: 792-796.
15. 15- Zondek B. (1959). Arborization of Cervical and Nasal Mucus and Saliva. Obstetrics and Gynecology.13(4): 477-481.
16. 16- Reinhard J, Lacey MJ, Ibarra F, Schroeder FC, Kaib M, Lenz M. (2002). Hydroquinone: A General Phagostimulating Pheromone İn Termites. Journal of Chemical Ecology. 28: 1–14.
17. 17-Sankar R, Archunan G, Habara Y. (2007). Detection Of Oestrous-Related Odour İn Bovine (Bos Taurus) Saliva: Bioassay Of İdentified Compounds. Animal. 1(9):1321-1327.
18. 18- Kirkman RJ. (1997). Approaches For İncorporating Ovulation Detection Devices And Home Kits İnto Learning Nfp - İmplications For Service Delivery. Adv Contracept. 13: 269-272.
19. 19- Skalova I, Fedorova T, Brandlova K. (2013). Short Communication Saliva Crystallization in Cattle: New Possibility for Early Pregnancy Diagnosis. Agricultura tropica et subtropica. 46(3): 102-104.
20. 20- Priya BS, Pushpaja M, Maruthy KN. (2020). Does The Salivary Fern Pattern Determine Fertile Period İn Reproductive Female?. Clinical Epidemiology and Global Health. 8(3): 698-701.
21. 21-Salmassi A, Schmutzler AG, Püngel F, Schubert M, Alkatout I, Mettler L. (2013). Ovulation Detection İn Saliva, İs İt Possible. Gynecologic And Obstetric İnvestigation. 76(3): 171-176.
22. 22- Gnanamuthu G, Rameshkumar K. (2015). Determination Of Estrus İn Umblachery Cattle (Bos indicus) By Salivary Fern Pattern. History.13(41): 68-78.
23. 23- Surla GN, Hebbar A, Kumar V, et al. (2021). Validation Of Salivary Ferning Based Estrus İdentification Method İn A Large Population Of Water Buffaloes (Bubalus bubalis) Using Foldscope. Reproductive Biology. 21(3):100528.