Comparative Diagnosis of Caseous Lymphadenitis in Sheep by Molecular and ELISA Method

Öz

In this study; It is aimed to reveal the diagnosis of Corynebacterium. pseudotuberculosis (C. pseudotuberculosis), which is the etiological agent of Caseous Lymphadenitis (CLA), in sheep in the Diyarbakir region using bacteriological culture, PCR and ELISA techniques, to determine the specificity and sensitivity of the tests with isolation findings, and thus to demonstrate their usability in the routine diagnosis of the disease. The material of the study consisted of 2650 Akkaraman sheep from 11 different herds in different regions of Diyarbakır. Among the sheep undergoing health screening, 90 sick sheep with CLA symptoms and one or more of the superficial lymph nodes with abscess were identified and included in the study. Blood samples and swap samples were taken from the abscess contents. Bacteriological culture and PCR analyzes were performed on swap samples and ELISA analyzes were performed on blood samples for the diagnosis of the causative agent. According to PCR analysis, 26 (28.9%) positive, 64 (71.1%) negative, 17 (18.9%) positive ELISA analysis, 73 (81.1%) negative, and 12 (13.3%) bacteriological cultures were found in sheep in Diyarbakir region. While C. pseudotuberculosis was isolated in 12, Staphylococcus aureus (S. aureus) in 16, Streptococ spp in 8, and Escherichia coli (E. coli) in 6, no growth was observed in 48 (53.3%) cultures appeared not to be. When PCR, ELISA and bacteriological culture results were compared, PCR was found to be a more definitive diagnostic test (p<0.000). Considering the isolation results, the sensitivity of PCR compared to ELISA was 100% and the specificity was 65%. As a result, it was concluded that CLA is seen in the sheep of the region and therefore, control and eradication studies should be carried out in order to prevent this disease in the region.

Anahtar Kelimeler

• Corynebacterium pseudotuberculosis
• Sheep
• Caseous Lymphadenitis
• PCR

Koyunlarda Kazeöz Lenfadenitisin Moleküler ve ELISA Yöntemiyle Karşılaştırmalı Teşhisi

Öz

Bu çalışmada; Diyarbakır yöresindeki koyunlarda Kazeöz Lenfadenitis’in (KLA) etiyolojik ajanı olan Corynebacterium. pseudotuberculosis (C. pseudotuberculosis)’in bakteriyolojik kültür, PCR ve ELISA teknikleri kullanılarak tanısının ortaya konulması, izolasyon bulguları ile testlerin özgüllük ve duyarlılıklarının belirlenmesi ve böylece hastalığın rutin teşhisinde kullanılabilirliklerinin ortaya konulması amaçlanmıştır. Çalışmanın materyalini Diyarbakır’ın farklı bölgelerinde olmak üzere 11 ayrı sürüden toplamda 2650 adet Akkaraman koyun oluşturdu. Sağlık taramasından geçirilen koyunların içinden 90 adet KLA semptomları gösteren ve yüzeysel lenf yumrularından bir veya birkaç tanesi birden apseleşmiş hasta koyunlar tespit edilerek çalışmaya dahil edildi. Kan örnekleri ve apse içeriğinden swap örnekleri alındı. Etkenin tanısı için, swap örneklerinden bakteriyolojik kültür ve PCR analizleri, kan örneklerinden ise ELISA analizleri yapıldı. Diyarbakır yöresindeki koyunlarda PCR analizlerine göre 26 (%28.9) pozitif, 64 (%71.1) negatif, ELISA analizinde 17 (%18.9) pozitif, 73 (%81.1) negatif ve bakteriyolojik kültürde 12 (%13.3)’sinde C. pseudotuberculosis, 16’sından Staphylococcus aureus (S. aureus), 8’inde Streptococ spp. ve 6’sında Escherichia coli (E. coli) kolonileri izole edilirken 48 (%53.3) kültürde hiç üreme olmadığı görüldü. PCR, ELISA ve bakteriyolojik kültür sonuçları karşılaştırıldığında PCR’ın daha belirleyici bir tanı testi olduğu görüldü (p<0.000). İzolasyon sonuçları dikkate alındığında KLA’nın tanısında PCR’ın ELISA’ya oranla duyarlılığı %100, özgüllüğü ise %65 olarak tespit edildi. Sonuç olarak KLA’nın bölge koyunlarında görüldüğü ve bu nedenle bölgede bu hastalığın önlenmesi için kontrol ve eradikasyon çalışmalarının yapılması gerektiği kanaatine varıldı.

Anahtar Kelimeler

• Corynebacterium pseudotuberculosis
• Kazeöz Lenfadenitis
• Koyun
• PCR
• Corynebacterium pseudotuberculosis
• Sheep
• Caseous Lymphadenitis

Kaynakça

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