Serum Immunoglobulin and Nitric Oxide Levels in Calves with Arthritis and Omphalitis

Abstract

Omphalitis and arthritis are diseases that are frequently encountered in calves and cause serious economic losses. In this study, it was aimed to determine serum immunoglobulin and NO levels in calves with omphalitis and arthritis and to examine the changes in these parameters compared to healthy calves. The animal material of this study consisted of calves with arthritis (n=20) and omphalitis (n=20) and calves (control group n=15) with clinically healthy from Van region. Clinical and radiological examinations were carried out, and blood samples were collected. Immunoglobulin A (IgA), immunoglobulin M (IgM), immunoglobulin G (IgG), gamma-glutamyl transferase (GGT) and nitric oxide (NO) levels were determined by ELISA. In our study, mean blood serum IgA, IgM, IgG, GGT, and NO levels of calves with arthritis were found to be 0.55 mg/ml, 4.63 mg/ml, 4.33 mg/ml, 29.63 ng/ml, and 624.1 µmol/l, respectively. Mean blood serum IgA, IgM, IgG, GGT and NO levels of calves with omphalitis were 0.52 mg/ml, 4.39 mg/ml, 2.04 mg/ml, 28.51 ng/ml, 538.75 µmol/L respectively. In conclusion, no significant statistical difference was observed between serum IgA, IgM, IgG, and GGT levels of animals diagnosed with omphalitis and arthritis. While in the omphalitis group, no statistical significance was observed for the NO serum levels, a statistical significance was present in the arthritis group.

Keywords

• Arthritis
• Calf
• Immunoglobulin
• Nitric oxide
• Omphalitis

Artritis ve Omfalitisli Buzağılarda Serum İmmünoglobulin ve Nitrik Oksit Seviyeleri

Öz

Omfalitis ve artritis buzağılarda sıklıkla karşılaşılan ve ciddi ekonomik kayıplara sebep olan hastalıklardandır. Bu çalışmada omfalitisli ve artiritisli buzağılarda serum immünoglobulin ve NO düzeylerinin belirlenmesi ve sağlıklı buzağılara göre bu parametrelerdeki değişimlerin incelenmesi amaçlanmıştır. Bu çalışmanın hayvan materyalini Van ili ve çevresinden sağlanan 20 artritisli ve 20 omfalitisli değişik ırk, yaş ve cinsiyette buzağı (çalışma grubu) ile klinik olarak sağlıklı 15 buzağı (kontrol grubu) oluşturmuştur. Hayvanların klinik ve radyolojik muayeneleri yapılarak kan örnekleri toplandı. Kan örneklerinden, immünoglobulin A (IgA), immünoglobulin M (IgM), immünoglobulin G (IgG), gama glutamil transferaz (GGT) ve nitrik oksit (NO) düzeyleri ELİSA cihazı ile tespit edildi. Çalışmamızda artritisli buzağıların ortalama kan serum IgA, IgM, IgG, GGT ve NO seviyeleri sırasıyla 0.55 mg/ml, 4.63 mg/ml, 4.33 mg/ml, 29.63 ng/ml, 624.1 µmol/L olarak bulunmuştur. Omfalitisli buzağıların ortalama kan serum IgA, IgM, IgG, GGT ve NO seviyeleri sırasıyla 0.52 mg/ml, 4.39 mg/ml, 2.04 mg/ml, 28.51 ng/ml, 538.75 µmol/L olarak bulunmuştur. Sonuç olarak, omfalitis ve artritis tanısı konan hayvanların serum IgA, IgM, IgG ve GGT seviyelerinde istatistiki olarak anlamlı bir fark gözlemlenmemiştir. Serum NO seviyelerinde ise omfalitis grubunda istatistiki olarak anlamlı bir fark gözlemlenmezken, artritis grubunda anlamlı bir fark gözlemlenmiştir.

Anahtar Kelimeler

• Artritis
• Buzağı
• İmmünoglobulin
• Nitrik oksit
• Omfalitis

Kaynakça

1. Wernery U. (2001). Camelid Immunoglobulins and Their Importance for the New-Born – A Review. J Vet Med B. 48 (8): 561–568.
2. Çakıroğlu D, Meral Y, Pekmezci D, Onuk EE, Gökalp G. (2010). Yeni Doğan Buzağılarda Çeşitli Hematolojik ve Biyokimyasal Parametreler ile Kolostral İmmun Globulinler Arasındaki İlişkinin Belirlenmesi. FÜ Sağ Bil Vet Derg. 24 (1): 43–46.
3. Butler JE. (1969). Bovine Immunoglobulins: A Review. J Dairy Sci. 52 (12): 1895–1909.
4. Parish SM, Tyler JW, Besser TE, Gay CC, Krytenberg D. (1997). Prediction of Serum IgG1 Concentration in Holstein Calves Using Serum Gamma Glutamyltransferase Activity. J Vet Intern Med. 11 (6): 344–347.
5. Morrill KM, Tyler HD. (2012). Two Methods to Determine IgG Concentration in Calf Serum. Iowa State University Animal Industry Report. 9 (1).
6. Şahal M, Terzi OS, Ceylan E, Erdal K. (2018). Buzağı İshalleri ve Korunma Yöntemleri. Lalahan Hay Araşt Enst Derg. 58 (3): 41–49.
7. Jackson P. (1999). Treatment of Septic Arthritis in Calves. In Pract. 21 (10): 596–601.
8. Desrochers A, Francoz D. (2014). Clinical Management of Aeptic Arthritis in Cattle. Vet Clin North Am Food Anim Pract. 30 (1): 177–203.

9. Bozukluhan K, Merhan O, Öğün M, Cihan M, Gökçe G. (2016). Omfalitisli Buzağılarda Bazı Oksidatif Stres Parametre Düzeylerinin Belirlenmesi. FÜ Sağ Bil Vet Derg. 30 (2): 79–81.
10. Thompson JC, Pauli J V. (1981). Colostral Transfer of Gamma Glutamyl Transpeptidase in Calves. N Z Vet J. 29 (12): 223–226.
11. Erbaş D, Anıl A, Kösebalaban Ş. (1989). Vitamin E ve Selenyum’un Dietil Nitrozamin Hepato Toksisitesine Karşı Etkileri. Gazi Üniversitesi Diş Hekimliği Fakültesi Dergisi. 6 (1): 9–14.
12. Cerutti A, Chen K, Chorny A. (2011). Immunoglobulin Responses at The Mucosal Interface. Annu Rev Immunol. 29 273.
13. Çekmen MB, Turgut M, Türköz Y, Aygün AD, Gözükara EM. (2001). Nitrik Oksit (NO) ve Nitrik Oksit Sentaz (NOS)’ın Fizyolojik ve Patolojik Özellikleri. Turkiye Klinikleri J Pediatr. 10 (4): 226–236.
14. Boulanger V, Zhao X, Lauzon K, Lacasse P. (2007). Effects of Nitric Oxide on Bovine Polymorphonuclear Functions. Can J Vet Res. 71 (1): 52–58.
15. Grover WM, Godden S. (2011). Efficacy of a New Navel Dip to Prevent Umbilical Infection in Dairy Calves. Bov Pract (Stillwater) 45 (1): 70–77.
16. Kozat S. (2019). Yenidoğan Buzağılarda Kolostrum Yönetiminin Önemi. Atatürk Üniversitesi Vet. Bil. Derg.,14(3): 343-353.
17. Constant C, Nichols S, Desrochers A, Babkine M, Fecteau G, Lardé H, et al. (2018). Clinical Findings and Diagnostic Test Results for Calves With Septic Arthritis: 64 cases (2009–2014). J Am Vet Med Assoc. 252 (8): 995–1005.
18. Dogan E, Yanmaz LE, Okumus Z, Kaya M, Senocak MG, Cengiz S. (2016). Radiographic, Ultrasonographic and Thermographic Findings in Neonatal Calves with Septic Arthritis: 82 cases (2006-2013). Atatürk Üniversitesi Vet Bil Derg. 11 (1): 6–12.
19. Bozukluhan K, Merhan O, Ogun M, Kurt B, Cihan M, Erkilic EE, et al. (2018). Investigation of Haptoglobin, Serum Amyloid A, and Some Biochemical Parameters in Calves With Omphalitis. Vet World. 11 (8): 1055–1058.
20. Murphy BM, Drennan MJ, O’Mara FP, Earley B. (2005). Cow Serum and Colostrum Immunoglobulin (IgG₁) Concentration of Five Suckler Cow Breed Types and Subsequent Immune Status of Their Calves. Irish Journal of Agricultural and Food Research. 44 (2): 205–213.
21. Blutt SE, Miller AD, Salmon SL, Metzger DW, Conner ME. (2012). IgA is Important for Clearance and Critical for Protection From Rotavirus Infection. Mucosal Immunol. 5 (6): 712–719.
22. Akbulut Ö, Bayram B, Yanar M, Keleş S. (2003). Serum Immunoglobulin Concentrations of Brown Swiss and Holstein Friesian Calves and Their Relationship with Growth Characteristics / Esmer ve Siyah Alaca Buzağıların Serum İmmunoglobulin Konsantrasyonları ve Bunların Büyüme ve Gelişme Özellikleri ile İliş. Atatürk Üniv Ziraat Fak Derg. 34 (2): 157 – 159.
23. Beard LJ, Ferris L, Ferrante A. (1990). Immunoglobulin G Subclasses and Lymphocyte Subpopulations and Function in Osteomyelitis and Septic Arthritis. Acta Paediatr Scand. 79 (6–7): 599–604.
24. Bloom KA, Chung D, Cunningham-Rundles C. (2008). Osteoarticular Infectious Complications in Patients with Primary Immunodeficiencies. Curr Opin Rheumatol. 20 (4): 480–485.
25. Sakınıene E, Bremell T, Tarkowskı A. (1997). Inhibition of Nitric Oxide Synthase (NOS) Aggravates Staphylococcus Aureus Septicaemia and Septic Arthritis. Clin Exp Immunol. 110 (3): 370–377.
26. Weinberg JB, Lang T, Wilkinson WE, Pisetsky DS, St Clair EW. (2006). Serum, Urinary, and Salivary Nitric Oxide in Rheumatoid Arthritis: Complexities of Interpreting Nitric Oxide Measures. Arthritis Res Ther. 8 (5): R140.
27. McInnes IB, Leung B, Wei XQ, Gemmell CC, Liew FY. (1998). Septic Arthritis Following Staphylococcus Aureus Infection in Mice Lacking Inducible Nitric Oxide Synthase. J Immunol. 160 (1): 308–315.