Alabalık Yemlerine Farklı Oranlarda İlave Edilen Siyah Asker Sineği Larvası (Hermetia illucens)‘nın Büyüme Performansı ve Bazı Kan Parametreleri Üzerine Etkileri

Yıl 2023, , 27 - 32, 30.06.2023

Fatih Akdemir

https://doi.org/10.47027/duvetfd.1271625

Öz

Bu çalışmada, Gökkuşağı alabalığı (Oncorhynchus mykiss) yemlerine farklı düzeylerde katılan dondurulmuş siyah asker sineği larvası (Hermetia illucens)’nın büyüme performansı ve bazı kan parametreleri üzerine olan etkileri araştırıldı. Her grupta 20 toplamda 80 adet balık olacak şekilde balıklar tartılıp boyları ölçüldükten sonra 4 gruba ayrıldı. Gruplar; canlı ağırlığın (CA) %1.5‘i bazal diyetle beslenen
kontrol grubu, CA’ın %1.5‘i larva ile beslenen larva grubu, CA’ın %1.125‘i bazal diyet + CA’ın %0.375‘i larva ile beslenen L1 grubu ve CA’ın %0.75‘i bazal diyet + CA’ın %0.75‘i larva ile beslenen L2 grubu şeklinde oluşturuldu. Çalışmanın başında ve 21 günlük süre sonunda ağırlık ve boy ölçümleri yapıldı. Çalışma sonunda bütün balıklar kesilerek kan örnekleri alınıp karkas ağırlıkları ve karkas verimleri belirlendi. L1
ve L2 gruplarının kontrol ve larva gruplarına göre yem tüketimi, canlı ağırlık artışı, yemden yararlanma oranı, karkas ağırlığı ve karkas verimi gibi büyüme parametreleri açısından daha iyi olduğu görüldü (P<0.05). Kan örneklerinde yapılan analizlerde LYM (Lenfosit, %), MID (Monosit, %), MCV (Ortalama Eritrosit Hacmi), MCH (Hücre hemoglobin ortalaması), RDW-SD (Kırmızı kan hücresi dağılım genişliği standart sapma), RDW-CV (Kırmızı kan hücresi dağılım genişliği-varyasyon katsayısı) ve PLT (Trombosit) değerlerinde gruplar arasında istatistiksel farklılık olduğu bulundu (P<0.05). Sonuç olarak, alabalıkları bazal diyete ilave olarak dondurulmuş siyah asker sineği larvası ile beslemenin büyüme performansında anlamlı düzeyde iyileştirmeler yaptığı görüldü. Ayrıca, balıkların kan yapımı hücreleri ile ilgili çok belirgin iyileştirmeler yapmamasına rağmen savunma sistemini destekleyici yönde etkiler yaptığı görüldü. Bu çalışma ile alabalıkların beslenmesinde larvaların tek başına kullanımından ziyade, bazal diyetle belli oranlarda karıştırılarak beslemenin daha faydalı olacağı söylenebilir.

Anahtar Kelimeler

Büyüme performansı, Gökkuşağı alabalığı, siyah asker sineği larvası

Destekleyen Kurum

İnönü Üniversitesi

Proje Numarası

FLO-2018-1113

Teşekkür

Bu çalışma, İÜBAP Koordinatörlüğünce Lisans Öğrencisi Katılımlı FLO-2018-1113 nolu proje ile desteklenmiş olup 3. Uluslararası Karadeniz Modern Bilimsel Araştırmalar Kongresi (23-24 Mart, 2023, Samsun, Türkiye)‘nde sözlü olarak sunulmuştur. Yazar hem adı geçen koordinatörlüğe hem de kongre düzenleme kuruluna teşekkür eder.

The Effects of Black Soldier Fly Larva (Hermetia illucens) Added to Trout Feed in Different Proportions on Growth Performance and Some Blood Parameters

Öz

In this study, the effects on growth performance and some blood parameters of frozen black soldier fly larva (Hermetia illucens) supplemented with different levels in rainbow trout (Oncorhynchus mykiss) diets were investigated. A total of 80 fish were divided into 4 groups after weighing and measuring their lengths, with 20 in each group. The groups were formed as follows: 1.5% of live wei ght (LW) basal diet fed control group, 1.5% of LW larva fed larvae group, 1.125% of LW basal diet + 0.375% of LW L1 group fed larvae and 0.75% of LW basal diet + 0.75% of LW larval fed L2 group. Weight and length measurements were made at the beginning of the study and at the end of the 21-day period. At the end of the study, all fish were slaughtered and blood samples were taken and carcass weights and carcass yields were determined. It was observed that L1 and L2 groups were better than control and larval groups in terms of growth parameters such as feed consumption, live weight gain, feed conversion ratio, carcass weight and carcass yield (P<0.05). Statistical differences were found between the groups in the analysis of blood samples in LYM(%), MID(%), LYM, MCV, MCH, RDW-SD, RDW-CV and PLT values. As a result, it was observed that feeding the trout with frozen black soldier fly larvae in addition to the basal diet significantly improved the growth performance. In addition, although it did not make significant
improvements in the blood-forming cells of fish, it made impacts to support the defense system. With this study, it can be said that it would be more beneficial to feed the trout by mixing them with the basal diet in certain proportions rather than using the larvae alone.

Anahtar Kelimeler

Black soldier fly larva, growth performance, rainbow trout

Proje Numarası

FLO-2018-1113

Kaynakça

• Turan H, Kaya Y, Sönmez G. (2006). Balık Etinin Besin Değeri ve İnsan Sağlığındaki Yeri. Ege JFAS. 23(3): 505-508.
• Oliva-Teles A, Enes P, Peres H. (2015). Replacing Fishmeal and Fish Oil in Industrial Aquafeeds for Carnivorous Fish. In: Davis DA, Editor. Feed and Feding Practice in Aquaculture. Cambridge: Woodhead Publishing. 203–233.
• FAO. (2016). The State of World Fisheries and Aquaculture: Contributing to Food Security and Nutrition for all. Food and Agriculture Organization of the United Nations, Rome.
• Raman SS, Stringer LC, Bruce NC, Chong CS. (2022). Opportunities, Challenges and Solutions for Black Soldier Fly Larvae-based Animal Feed Production. J Clean Prod. 373: 133802.
• Tomberlin JK, van Huis A, Benbow ME, et al. (2015). Protecting the Environment Through Insect Farming as a Means to Produce Protein for Use as Livestock, Poultry, and Aquaculture Feed. J Insects Food Feed. 1(4): 307-309.
• de Marco M, Martínez S, Hernandez F, et al. (2015). Nutritional Value of Two Insect Larval Meals (Tenebrio Molitor and Hermetia Illucens) for Broiler Chickens: Apparent Nutrient Digestibility, Apparent Ileal Amino Acid Digestibility And Apparent Metabolizable Energy. Anim Feed Sci Technol. 209: 211-218.
• Renna M, Schiavone A, Gai F, et al. (2017). Evaluation of The Suitability of A Partially Defatted Black Soldier Fly (Hermetia illucens) Larvae Meal as Ingredient for Rainbow Trout (Oncorhynchus Mykiss, Walbaum) Diets. J Anim Sci Biotechnol. 8(1): 57.
• Aisyah HN, Athirah ZAR, Hanani WR, et al. (2022). The Effect of Feeding Black Soldier fly Larvae on Growth Performance, Protein, and Fat Content of Red Hybrid Tilapia (Oreochromis spp.). Vet World. 15(10): 2453-2457.
• Jozefiak D, Engberg RM. (2015). “Insect as Poultry Feed” 20th European Symposium on Poultry Nutrition, 24-27 August, Prague, Czech Republic.
• Sheppard DC, Newton GL, Thompson SA, Savage S. (1994). A Value Added Manure Management System Using The Black Soldier Fly. Bioresour Technol. 50(3): 275-279.
• Stamer A, Wessels S, Neidigk R, Hoerstgen-Schwark G. (2014). Black Soldier Fly (Hermetia illucens) Larvae-Meal As An Example for A New Feed Ingredients’ Class in Aquaculture Diets. 4 th ISOFAR Scientific Conference. Building Organic Bridges, at the Organic World Congress, 13 - 15 October, Istanbul, Turkey (eprint ID 24223).
• Charlton AJ, Dickinson M, Wakefield ME, et al. (2015). Exploring the Chemical Safety of Fly Larvae as a Source of Protein for Animal Feed. J Insects Food Feed. 1(1): 7-16.
• Nairuti RN, Musyoka SN, Yegon MJ, Opiyo MA. (2022). Utilization of Black Soldier Fly (Hermetia illucens L.) Larvae as a Protein Source for Fish Feed - A Review. Aquac Stud. 22(2): AQUAST697.
• Tomberlin JK, Adler PH, Myers HM. (2009). Development of the Black Soldier Fly (Diptera: Stratiomyidae) in Relation to Temperature. Environ Entomol. 38(3): 930-934.
• Nguyen TTX, Tomberlin JK, Vanlaerhoven S. (2015). Ability of Black Soldier Fly (Diptera: Stratiomyidae) Larvae to Recycle Food Waste. Environ Entomol. 44: 406-410.
• Sheppard DC, Newton GL. (2000). Valuable By-products of a Manure Management System Using the Black Soldier Fly. Pages. 35-39 in Animal, Agricultural and Food Processing Wastes, Proceedings of the 8th International Symposium. ASAE, St Joseph, MO.
• Widjastuti T, Wiradimadja R, Rusmana D. (2014). The Effect of Substitution of Fish Meal by Black Soldier Fly (Hermetia illucens) Maggot Meal in the Diet on Production Performance of Quail (Coturnix coturnix japonica). Sci Papers Ser D Anim Sci. 42: 125–129.
• Henry M, Gasco L, Piccolo G, Fountoulaki E. (2015). Review on the Use of Insects in the Diet of Farmed Fish: Past and Future. Anim Feed Sci Technol. 203: 1-22.
• Newton GL, Sheppard DC, Watson DW, et al. (2005). The Black Soldier Fly, Hermetia illucens, as a Manure Management/Resource Recovery Tool. In Symposium on The State of The Science of Animal Manure and Waste Management, pp. 5-7.
• Leong SY, Kutty SRM, Malakahmad A, Tan CK. (2016). Feasibility Study of Biodiesel Production Using Lipids of Hermetia illucens Larva Fed with Organic Waste. Waste Manag. 47: 84-90.
• St-Hilaire S, Cranfill K, McGuire MA, et al. (2007a). Fish Offal Recycling by the Black Soldier Fly Produces a Foodstuff High in Omega-3 Fatty Acids. J World Aquac Soc. 38: 309.
• Veldkamp T, van Duinkerken G, van Huis A, et al. (2012). Insects as a Sustainable Feed Ingredient in Pig and Poultry Diets – A Feasibility Study, Report 638 - Wageningen Livestock Research.
• Bondari K, Sheppard DC. (1981). Soldier Fly Larvae as Feed in Commercial Fish Production. Aquac. 24: 103-109.
• Ng WK, Liew FL, Ang LP, Wong KW. (2001). Potential of Mealworm (Tenebrio molitor) as an Alternative Protein Source in Practical Diets for African Catfish, Clarias gariepinus. Aquac Res. 32 (Supplement 1): 273-280.
• St-Hilaire S, Sheppard C, Tomberlin JK, et al. (2007b). Fly Prepupae as a Feedstuff for Rainbow Trout, Oncorhynchus mykiss. J World Aquac Soc. 38: 59-67.
• Doğankaya L. (2017). Gökkuşağı Alabalığı Yavru Yemlerinde Balık Unu Yerine Süper Kurt (Zophobas morio) Unu İkamesinin Büyüme Performansına Etkileri. Turkish J Aqua Sci. 32(1): 1-7.
• Lock E, Arsiwalla T, Waagbø R. (2016). Insect larvae Meal as an Alternative Source of Nutrients in the Diet of Atlantic Salmon (Salmo salar) Posts Molt. Aquac Nutr. 22 (6): 1202-1213.
• Altinterim B, Danabas D, Aksu O. (2018). The effects of Common Yarrow (Achillea millefolium Linnaeus), Cinnamon (Cinnamomum zeylanicum Blume) and Rosemary (Rosemarinus officinalis Linnaeus) Hydrosols on the Some İmmunological and Hematological Parameters of Common carp (Cyprinus carpio L., 1758). Cell Mol Biol. 64(14): 19–24.
• Altınterim B, Kutluyer F, Aksu O. (2018). Effects of Different Plant Oils Having Different Oxygen Radical Absorbance Capacity (ORAC) on Hematological Parameters of Rainbow Trout (Oncorhynchus mykiss) at High Stocking Density. Ataturk Univ Vet. 13(1): 63-69.
• Altinterim B, Ozturk E, Kutluyer F, Aksu O. (2018). Effects of Green Tea (Camellia sinensis) Oil on Feed Utilization and Hametological Parameters of Rainbow trout (Oncorhynchus mykiss). Ataturk Univ. Vet. 13(2): 159-164.
• Abdel-Tawwab M, Khalil RH, Metwally AA, Shakweer MS, Khallaf MA, Abdel-Latif HM. (2020). Effects of Black Soldier Fly (Hermetia illucens) Larvae Meal on Growth Performance, Organssomatic Indices, Body Composition, and Hemato-biochemical Variables of European Sea Bass, Dicentrarchus Labrax. Aquac. 522: 735136.