Ratlarda Gebeliğin Erken Döneminde Ovaryum ve Oviduktta Beclin1 ile LC3’ün İmmunohistokimyasal İfadesi

Öz

Otofaji ile bağlantılı proteinler (Autophagy Related Proteins), Atg proteinleri olarak isimlendirilir. Otofajinin moleküler düzeyde düzenlenmesinde Atg6 (Beclin 1) kompleksi ve otofaji sırasında otofagozomda sitozolik proteinleri, organelleri, sitoplazmik bileşenleri lize eden LC3 ise otofagozomal işaretleyicilerdendir. Otofaji ile ilgili süreçlerin izlenmesi gebelik metabolizmasının anlaşılmasında oldukça önemlidir. Yapılan çalışmada gebeliğin birinci periyodu içinde 5. günde ovaryum ve oviduktta Beclin 1, LC3 immunohistokimyasal olarak gösterilmiştir. Buna göre gebeliğin 5. gününde ovaryumda intertisyel hücreler, germinatif epitelde, folikül granüloza hücrelerinde ve teka interna/eksternada, oviduktta ise epitel hücrelerinde, Beclin 1, LC3’ün immunohistokimyasal olarak (+) liği ile otofajinin gebelikte artarak devam ettiği belirlenmiştir.

Anahtar Kelimeler

• Beclin 1
• gebelik
• lc3
• otofaji
• wistar albino rat

Immunohistochemical Expression of Beclin1 and Lc3 in the Ovary and Oviduct during Early Pregnancy in Rats

Öz

Proteins associated with autophagy are called (Autophagy related proteins) Atg proteins. Atg6 (Beclin 1) complex regulates autophagy at the molecular level and lyses cytosolic proteins, organelles and cytoplasmic components in the autophagosome during autophagy. LC3 is an autophagosomal marker. Monitoring autophagy-related processes is very important in understanding pregnancy metabolism. In this study, Beclin 1 and LC3 were immunohistochemically demonstrated in the ovary and oviduct on day 5 in the first pregnancy period. Accordingly, on the 5th day of pregnancy, it was determined that autophagy continued to increase in pregnancy with the immunohistochemical (+) of Beclin 1, LC3 in interstitial cells, germinative epithelium, follicle granulosa cells and theca interna/externa in the ovary and epithelial cells in the oviduct.

Anahtar Kelimeler

• Autophagy
• beclin 1
• lc3
• pregnancy
• wistar albino rat

Kaynakça

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