Melanopsis praemorsa (Linnaeus, 1758)’nın Manto Dokusu Üzerine Glifosatın Histopatolojik ve Ultrayapısal Etkileri

Öz

Bu çalışmanın amacı, tatlı su ekosistemlerindeki pestisit kirliliğini araştırmak ve M. praemorsa'nın biyoindikatör olarak potansiyelini değerlendirmektir. Çalışmada, 30 günlük bir maruz kalma süresince öldürücü olmayan glifosat konsantrasyonlarına maruz bırakılan tatlı su salyangozu Melanopsis praemorsa'nın (Linnaeus, 1758) manto dokusundaki histopatolojik ve ultrastrüktürel değişiklikler incelenmiştir. Deney dört gruptan oluşmuştur: bir kontrol grubu ve üç glifosat grubu (0,933 mg/L, 1,867 mg/L ve 4,666 mg/L). Her gruba rastgele 30 benzer büyüklükte salyangoz atandı ve manto dokuları maruziyetin 10., 20. ve 30. günlerinde örneklendi. Fiksasyon/işlemden sonra, dokular ışık mikroskobu ve TEM ile incelendi. Histopatolojik bulgular arasında epitel deskuamasyonu, kas liflerinin atrofisi, sillerin dejenerasyonu, lipit vakuolizasyonunun artması ve nekroz. Ultrastrüktürel düzeyde mitokondriyal dejenerasyon, nükleer anormallikler ve hücresel lizis gözlendi. Manto dokusundaki lezyonların şiddeti, glifosat konsantrasyonuna ve maruz kalma süresine bağlı olarak arttı

Anahtar Kelimeler

• Glifosat
• histopatolojik değişiklikler
• manto
• Melanopsis praemorsa
• ultrastrüktürel değişiklikler

Histopathological and Ultrastructural Effects of Glyphosate on the Mantle Tissue of Melanopsis praemorsa (Linnaeus, 1758)

Abstract

This study aimed to investigate pesticide pollution in freshwater ecosystems and to assess the potential of M. praemorsa as a bioindicator. Study examined the histopathological and ultrastructural changes in the mantle tissue of the freshwater snail Melanopsis praemorsa (Linnaeus, 1758) exposed to sublethal concentrations of glyphosate during a 30-day exposure. The experiment consisted of four groups: a control group and three glyphosate groups (0.933 mg/L, 1.867 mg/L, and 4.666 mg/L). Each group was randomly assigned 30 similarly sized snails. and mantle tissues were sampled on days 10, 20, and 30 of exposure. After fixation/processing, tissues were examined by light microscopy and TEM. Histopathological findings included epithelial desquamation, atrophy of muscle fibers, degeneration of cilia, increased lipid vacuolization, and necrosis. At the ultrastructural level, mitochondrial degeneration, nuclear abnormalities, and cellular lysis were observed. The severity of lesions in the mantle tissue increased depending on glyphosate concentration and exposure duration.

Keywords

• Glyphosate
• histopathological alterations
• mantle
• Melanopsis praemorsa
• ultrastructural changes

Kaynakça

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