Türkiye’nin Kars Bölgesinde İshalli Buzağılarda Mikroskopi ve Koproantijen Testi ile Cryptosporidiosisin Tespiti

Yıl 2025, Cilt: 18 Sayı: 2, 136 - 140, 31.12.2025

Neslihan Ölmez , Nilgün Aydın , Barış Sarı , Gencay Taşkın Taşçı , Enes Akyüz , Mert Sezer

https://doi.org/10.47027/duvetfd.1792067

Öz

Cryptosporidiosis, omurgalı hayvanlarda ishale neden olan paraziter bir hastalıktır. Yenidoğan hayvanlarda, özellikle buzağılarda sıklıkla görülür. Enfeksiyon dünya çapında yaygındır ve Türkiye’de çeşitli hayvan türlerinde bildirilmiştir. Bu çalışma, mikroskobik boyama ve koproantijen bazlı tespit tekniklerini kullanarak ishal olan buzağılarda Cryptosporidium spp. prevalansını değerlendirmek amacıyla yapılmıştır. Kars bölgesinde ishal olan yüz (100) buzağı incelenmiştir. Oositler flotasyon tekniği kullanılarak saflaştırılmış ve ardından modifiye asit dirençli yöntem (mAF) ile boyanmıştır. Tüm örnekler hızlı koproantijen testi ile de analiz edilmiştir. Mikroskobik inceleme, 100 numunenin 8’inde (%8) Cryptosporidium pozitifliği saptanırken, koproantijen tespiti ile 20 pozitif numune (%20) saptanmıştır.

Anahtar Kelimeler

Buzağı , Cryptosporidiosis , Cryptosporidium , Kars , Koproantijen test , Modifiye Asit-Fast

Etik Beyan

Bu çalışma için gerekli etik kurul onayı Kafkas Üniversitesi Yerel Hayvan Deneyleri Etik Kurulu'ndan 2025-117 karar numarası ile alındı.

Detection of Cryptosporidiosis in Diarrheic Calves in the Kars Region of Türkiye Using Microscopy and Coproantigen Test

Öz

Cryptosporidiosis is a parasitic disease that causes diarrhea in vertebrate animals. It is frequently observed in neonatal livestock, particularly in calves. The infection is globally widespread and has been reported in various animal species in Türkiye. This study aimed to assess the prevalence of Cryptosporidium spp. in calves suffering from diarrhea using both microscopic staining and coproantigen-based detection techniques. One hundred (100) calves suffering from diarrhea were studied from in Kars region. Oocysts were purified using the flotation technique and subsequently stained with the modified acid-fast method (mAF). All samples were also analyzed using a rapid coproantigen test. Microscopic examination revealed that 8 out of 100 samples (8%) were positive for Cryptosporidium, while coproantigen detection identified 20 positive samples (20%).

Anahtar Kelimeler

Calves , Cryptosporidiosis , Cryptosporidium , Modified Acid-Fast , Coproantigen rapid test , Kars.

Etik Beyan

The ethical committee approval required for this study was obtained from the Kafkas University Local Animal Experiments Ethics Committee with decision number 2025-117.

Destekleyen Kurum

There was no funding from any organization to conduct this research.

Kaynakça

• Thomson S, Hamilton CA, Hope JC, et al. (2017). Bovine cryptosporidiosis: impact, host-parasite interaction and control strategies. Vet Res, 48(1):42.
• Santín M, Trout JM, & Fayer R (2008). A longitudinal study of cryptosporidiosis in dairy cattle from birth to 2 years of age. Vet Parasitol, 155(1-2):15-23.
• Ryan U, Zahedi A, Feng Y, & Xiao L (2021). An update on zoonotic Cryptosporidium species and genotypes in humans. Animals (Basel), 11(11)
• Tůmová L, Ježková J, Prediger J, et al. (2023). Cryptosporidium mortiferum n. sp. (Apicomplexa: Cryptosporidiidae), the species causing lethal cryptosporidiosis in Eurasian red squirrels (Sciurus vulgaris). Parasit Vectors, 16(1):235.
• Fayer R, Santín M, & Trout JM (2008). Cryptosporidium ryanae n. sp. (Apicomplexa: Cryptosporidiidae) in cattle (Bos taurus). Vet Parasitol, 156(3-4):191-198.
• Xiao L (2010). Molecular epidemiology of cryptosporidiosis: an update. Exp Parasitol, 124(1):80-89.
• Santín M, Trout JM, Xiao L, Zhou L, Greiner E, & Fayer R (2004). Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves. Vet Parasitol, 122(2):103-117.
• Díaz P, Navarro E, Remesar S, et al. (2021). The age-related Cryptosporidium Species distribution in asymptomatic cattle from North-Western SPAIN. Animals (Basel), 11(2)
• Sayın İpek DN, & Sarı B (2024). Occurrence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in lambs and calves in Southeastern Anatolia, Turkey. Egypt J Vet Sci, 55(2):599-606.
• Renaud D, Rot C, Marshall J, & Steele M (2021). The effect of Cryptosporidium parvum, rotavirus, and coronavirus infection on the health and performance of male dairy calves. J Dairy Sci, 104(2):2151-2163.
• Sari B, Aktaş MS, & Arslan MO (2008). The prevalence of Cryptosporidium spp. in calves in Erzurum province. Turk Parazitol Derg, 32(2):116-119.
• Reijnders M, van Roosmalen M, Holtslag H, et al. (2025). The evaluation of the efficacy of a novel subunit vaccine in the prevention of Cryptosporidium parvum-caused diarrhoea in neonatal calves. Animals, 15(2):132.
• Chen Y, Huang J, Qin H, Wang L, Li J, & Zhang L (2023). Cryptosporidium parvum and gp60 genotype prevalence in dairy calves worldwide: a systematic review and meta-analysis. Acta Trop, 240:106843.
• Değerli S, Çeliksöz A, Kalkan K, & Özçelik S (2005). Prevalence of Cryptosporidium spp. and Giardia spp. in cows and calves in Sivas. Turk J Vet Anim Sci, 29(4):995-999.
• Gündüz N, & Arslan M (2017). Determining the prevalence of Cryptosporidium infections with acid fast staining and ELISA in calves at the Kars Province of Turkey. Turk Parazitol Derg, 41(1):5-8.
• Karakavuk M, Can H, Can Ş, Karakavuk T, Döşkaya M, & Değirmenci Döşkaya A (2024). Development of a "Rapid-Crypto Colorimetric LAMP Test" to detect cryptosporidiosis in feces of newborns calves. Acta Parasitol, 69(1):691-699.
• Papini R, Bonelli F, Montagnani M, & Sgorbini M (2018). Evaluation of three commercial rapid kits to detect Cryptosporidium parvum in diarrhoeic calf stool. Ital J Anim Sci, 17(4):1059-1064.
• Ölmez N, & Sarı B (2025). Neglected parasite of rats and mice: Cryptosporidium. Rats, 3(1):15-17.
• Geurden T, Claerebout E, Vercruysse J, & Berkvens D (2008). A Bayesian evaluation of four immunological assays for the diagnosis of clinical cryptosporidiosis in calves. Vet J, 176(3):400-402.
• Muccio JL, Grooms DL, Mansfield LS, Wise AG, & Maes RK (2004). Evaluation of two rapid assays for detecting Cryptosporidium parvum in calf feces. J Am Vet Med Assoc, 225(7):1090-1092.
• Cho YI, & Yoon KJ (2014). An overview of calf diarrhea - infectious etiology, diagnosis, and intervention. J Vet Sci, 15(1):1-17.
• Klein D, Kern A, Lapan G, et al. (2009). Evaluation of rapid assays for the detection of bovine coronavirus, rotavirus A and Cryptosporidium parvum in faecal samples of calves. Vet J, 182(3):484-486.
• Martins S, Sousa S, Madeira de Carvalho L, Bacelar J, & Cannas da Silva J (2007). Prevalence of Cryptosporidium parvum infection in northwest Portugal dairy calves and efficacy of halofuginone lactate on the prevention of cryptosporidiosis. Cattle Practice, 15(2):152.
• Lee YJ, Ryu JH, Shin SU, & Choi KS (2019). Prevalence and molecular characterization of Cryptosporidium and Giardia in pre-weaned native calves in the Republic of Korea. Parasitol Res, 118(12):3509-3517.
• Yildirim A, Adanir R, Inci A, et al. (2020). Prevalence and genotyping of bovine Cryptosporidium species in the Mediterranean and Central Anatolia Region of Turkey. Comp Immunol Microbiol Infect Dis, 69:101425.
• Çelik ÖY, Şahin T, Çelik BA, et al. (2023). Prevalence and molecular characterization of Cryptosporidium spp. in calves in the Siirt Province, Türkiye. Acta Vet Brno, 92(3):243-250.
• Guven E, Avcioglu H, Balkaya I, Hayirli A, Kar S, & Karaer Z (2013). Prevalence of Cryptosporidiosis and molecular characterization of Cryptosporidium spp. in calves in Erzurum. Kafkas Üniv Vet Fak Derg, 19(6)
• Arslan MÖ, Gıcık Y, Erdoğan HM, & Sarı B (2001). Prevalence of Cryptosporidium spp. oocysts in diarrhoeic calves in Kars Province, Turkey. Turk J Vet Anim Sci, 25(2):161-164.
• Aydın F, Umur Ș, Gökçe G, Genç O, & Güler M (2001). The isolation and identification of bacteria and parasites from diarrhoeic calves in Kars District. Kafkas Üniv Vet Fak Derg, 7(1):7-14.
• Çitil M, Arslan M, Güneș V, & Erdoğan H (2004). The role of Cryptosporidium and Eimeria infections in diarrhoea of neonatal calves. Kafkas Üniv Vet Fak Derg, 10(1):59-64.
• Öztürkler O, Kızıltepe Ş, Alkan H, & Hacıömeroğlu S (2023). Investigation of some microbial and protozoon factors with rapid test kits in neonatal diarrheas in calves in Kars province. Onehealthjournal, 1(1):1-6.