N- Butil 2 Siyanoakrilatın Biyolojik Etkilerinin Araştırılması

Yıl 2020, Cilt: 3 Sayı: 1, 20 - 31, 21.04.2020

Oğuz Karahan Mahmut Balkan Erhan Hafız Emced Khalil

https://doi.org/10.33713/egetbd.699440

Öz

Amaç: N- Butil 2 Siyanoakrilat kullanılan bir cerrahi yapıştırıcıdır. Bu çalışmada N-Butil 2 siyanoakrilatın biyouyumluluk analizleri deneysel olarak değerlendirilmiştir.
Gereç Yöntem: Wistar Albino cinsi, sağlıklı 16 erkek (250 ± 5 gram) yetişkin rat kontrol ve çalışma gruplarına bölündü. Kontrol grubundan normal histolojik yapının belirlenmesi ve rutin fizyolojik izlem amaçlandı. Çalışma gruplarında 0.3 ml N-Butil 2 siyanoakrilat enjeksiyonu, bel ve sırt traş edilen grupta irritasyon ve sensitizasyon değerlendirmesi için subkutan yoldan, sağ femur bölgesi traş edilen grubunda sitotoksisite değerlendirmesi için intramüsküler yoldan ve vasküler uygulama grubunda sistemik toksisite değerlendirmesi için kuyruk ven enjeksiyonu yoluyla uygulandı. Yetmiş iki saatlik gözlem süresinden sonra ratlar sakrifiye edilerek, venöz endotel, kas, dermal ve epidermal dokular histopatolojik olarak incelendi.
Bulgular: Sadece intravenöz uygulamada belirgin endotel harabiyeti izlendi. Sensitizasyon, irritasyon, sitotoksisite gibi testlerde aşırı reaksiyon ve belirgin histopatolojik değişiklik izlenmedi.
Sonuç: Cerrahi bir yapıştırıcı olarak sadece N-Butil 2 siyanoakrilatın intravasküler olarak uygulanması, lokal ve sistemik etkiler açısından ileri düzeyde doku hasarına yol açtı. Ancak, bu deneysel model klinik kapsamlı çalışmalarla desteklenmelidir.

Anahtar Kelimeler

N-Butil 2 siyanoakrilat, biyouyumluluk, histopatoloji

Destekleyen Kurum

Bu çalışma Dicle Üniversitesi Sağlık Bilimleri Enstitüsü Tıbbi Biyoloji Anabilim Dalınca Oğuz KARAHAN 'a ait doktora tezi olarak hazırlanıp 10319698 numara ile sunulmuş 28.01.2020 tarihinde onaylanarak doktora tamamlandıktan sonra yayın aşamasına getirilmiştir.

Investigation of Biological Effects of N- Butyl 2 Cyanoacrylate

Öz

Aim: N-Butyl 2 cyanoacrylate is a frequently utilizing agent as a surgical adhesive. Biocompatibility analyses of N-Butyl 2 cyanoacrylate were evaluated in this study.
Material and Method: Wistar Albino genus 16 healthy adult male (250 ± 5 gram) rats were divided into control and study groups. Determination of normal histological structure and routine physiologic observation was aimed in control group. In the study groups, 0.3 ml N-Butyl 2 cyanoacrylate injection were applied, via subcutaneous route for irritation and sensitization evaluation in the waist and back shaved group, via intramuscular route for cytotoxicity evaluation in the right femoral shaved group, and via tail vein injection for systemic toxicity evaluation in vascular administration group. Rats were sacrificed after 72. hours observation period and venous endothelium, muscular, dermal and epidermal tissues were examined histopathologically.
Findings: Marked endothelial injury were detected in only intravenous administration. Extreme reaction and marked histopatholgical changes were not observed in regards of irritation, sensitization and cytotoxicity groups .
Conclusion: Only intravascular administration of N-Butyl 2 cyanoacrylate, as a surgical adhesive usage, was lead advanced tissue injury in regards of local and systemic effects. However, this experimental model should be supported with clinical comprehensive studies.

Anahtar Kelimeler

N-Butyl 2 cyanoacrylate, biocompatibility, histopathology

Kaynakça

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