Gökkuşağı Alabalığı (Oncorhynchus mykiss) ve Aynalı Sazan (Cyprinus carpio) Spermasının Glukoz Tabanlı Sulandırıcı ile Dondurulması

Öz

Bu çalışmada, gökkuşağı alabalığı (Oncorhynchus mykiss) ve aynalı sazan (Cyprinus carpio) spermasının dondurulması araştırıldı. Spermatolojik özelliklerin belirlenmesinin ardından, ejakulatlar glukoz tabanlı sulandırıcı ile sulandırıldı. Sulandırılan sperma 0,5 ml’lik payetlere çekilerek sıvı azot buharında (-196 °C) 10 dakika süre ile donduruldu ve sıvı azot içerisine aktarıldı. Payetler 30 °C su banyosunda 30 saniye süre ile çözüldü. Gökkuşağı alabalığında çözülme sonu en iyi motilite (%), canlılık süresi (s) ve döl verimi yüzdesi sırasıyla % 57, 96 s. ve % 68,5 olarak elde edilirken aynı özellikler aynalı sazanda sırasıyla % 64, 218 s. ve % 35 olarak belirlendi.

Anahtar Kelimeler

• Gökkuşağı alabalığı (Oncorhynchus mykiss), aynalı sazan (Cyprinus carpio), sperma, sulandırıcı, kryoprezervasyon, payet, motilite, döllenme

Cryopreservat on of Rainbow Trout (Oncorhynchus mykiss) and Mirror Carp (Cyprinus carpio) Sperm with Glucose Based Extender

Öz

In this study, cryopreservation of rainbow trout (Oncorhynchus mykiss) and mirror carp (Cyprinus carpio) spermatozoa was investigated. Following determination of the spermatological properties, the pooled ejaculates were diluted with glucose based extender. Diluted sperm was packaged in 0.5 ml straws and frozen in liquid nitrogen vapour (-196 °C) for 10 minutes and plunged into the liquid nitrogen. The straws were thawed in a water bath at 30 ºC for 30 seconds. In rainbow trout, the best post-thaw motility (%), post-thaw movement duration (s) and post-thaw fertilization results (%) were obtained as 57 %, 96 s. and 68.5 % while the same properties were determined as 64 %, 218 s. and 35 % in mirror carp respectively

Anahtar Kelimeler

• Rainbow trout (Oncorhynchus mykiss), mirror carp (Cyprinus carpio), sperm, extender, cryopreservation, straw, motility, fertilization

Kaynakça

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