First Case of Vibriosis Caused by Vibrio harveyi in Salema (Sarpa salpa) from a Public Aquarium

Abstract

In this study, moribund salema (Sarpa salpa) individuals from a public aquarium were investigated to identify the cause of disease. A minor scale loss and partial skin depigmentation were observed in the diseased fish. Internal investigation of fish revealed hyperemia in the liver and the intestines and haemorrhages in the muscle tissues. When the histopathological sections of tissues were examined, it was determined that the bacterial infection affected especially the gill epithelium, the intestinal mucosa, the heart muscle and hemopoietic tissues of the spleen. According to phenotypic and biochemical characteristics as well as SSU rRNA sequencing results, the isolated bacteria was Vibrio harveyi and consistently seen all the examined samples of visceral organs including kidney, spleen, liver and blood. However, we need to emphasize that Vibrio vulnificus and Pseudoalteromonas piscicida bacteria were also isolated from blood and spleen tissues of one sick fish among others.

Keywords

• Vibriosis,Vibrio harveyi,salema,Sarpa salpa,public aquarium

Bir Deniz Akvaryumunda Salpa Balıklarında (Sarpa salpa) Vibrio harveyi Enfeksiyonu Olgusu: ilk rapor

Öz

Bu çalışma, bir deniz akvaryumundan alınan hasta salpa balıklarında (Sarpa salpa) hastalığa neden olan etken veya etkenleri tanımlamak üzere yapılmıştır. Hasta balıkların vücudunda hafif şiddette deri renginde açılma ile birlikte pul kayıpları gözlenmiştir. İç bakıda, karaciğer ve bağırsak dokularının hiperemik ve kas dokusunun da hemorajik olduğu tespit edilmiştir. Doku kesitleri histopatolojik olarak incelendiğinde, enfeksiyonun; özellikle solungaç epitelyumu, bağırsak mukozası, kalp kası ve dalağın hematopoetik dokusunu etkilediği belirlenmiştir. Fenotipik ve biyokimyasal özellikleri ve SSU rRNA dizileme sonuçlarına göre; incelenen tüm iç organlardan (böbrek, dalak, karaciğer) ve kan dokusundan Vibrio harveyi bakterisi izole edilmiştir. Buna ek olarak, incelenen balıklardan sadece birinin kan dokusundan ve dalağından alınan örneklerde Vibrio vulnificus ve Pseudoalteromonas piscicida bakterileri de izole edilmiştir.

Anahtar Kelimeler

• Vibriozis,Vibrio harveyi,salema,Sarpa salpa,deniz akvaryumu

Kaynakça

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