Lactococcus garvieae Suşlarının Antimikrobiyal Duyarlılıklarının Belirlenmesi

Yıl 2005, Cilt: 1 Sayı: 1, - , 04.01.2005

Ayşegül Kubılay Soner Altun Gülşen Uluköy Öznur Dıler

Öz

Bu çalışmada, 9 farklı Lactococcus garveiae suşlanmn Mueller-Hinton agarda disk diffüzyon tekniği ile ATB VET (Biomerieux 14 289) strip sistemi kullanılarak antimikrobiyal duyarlılıklarının yanısıra E testi (AB BIODISK) ile de Eritromycin antibiyotiğinin MIK(Minimal inhibitör konsantrasyonu) değerleri incelenmiştir. L.garvieae suşlarının; disk diffüzyon testi ve ATB VET sistemine göre Amoxicillin+clavulanic acid, Ampicillin, Enrofloxacin, Vancomycin, Tetracycline, Doxycycline, Chloramphenicol, Erytromycine, Nitrofurantoin, Cephalotin, Amoxicillin, Cefoperazon, Spectinomycin, Pristinamycin, Cotrimoxazol, antibiyotiklerine duyarlı oldukları, Kanamycin, Cefuroxime, Lincomycin, Penicillin, Ofloxacin, Norfloxacin,Ciprofloxacin Ceftriaxone, Clindamycine, Vancomycin, Oxacillin, Streptomycin, Trimethoprim+ Sulfamethoxazole, Gentamicin, Apramycin, Tylosin, Colistin, Sulfamethizol, Flumequin, Oxolinic asit antibiyotiklerine dirençli oldukları tespit edilmiştir. L. garvieae suşlarında eritromisin antibiyotiğinin MIK değerleri 0,032-0,125 gg/mL olarak tespit edilmiştir. Elde edilen bu değerlere göre; suşların eritromisine duyarlı olduğu görülmüştür.

Anahtar Kelimeler

Lactococcus garveiae, antimikrobiyal duyarlılık, minimum inhibitör konsantrasyonu (MIK)

The Determination of Antimicrobial Susceptibilities of Lactococcus garvieae Strains

Öz

This study was to investigated antimicrobial susceptibilities by disc diffusion method on MuellerHinton agar and ATB VET (14 289 Biomerieux) strip system and also the value the minimum inhibitory concentration (MIC) of Eritromycine antibiotic by Etest (ABBIODISK) in 9 strains of Lactococcus garvieae. L.garvieae strains were determined as sensitive to Amoxicillin+clavulanic acid, Ampicillin, Enrofloxacin, Vancomycin, Tetracycline, Doxycycline, Chloramphenicol, Erytromycine, Nitrofurantoin, Cephalotin, Amoxicillin, Cefoperazon, Spectinomycin, Pristinamycin, Cotrimoxazol, and resistant to Kanamycin, Cefuroxime, Lincomyc n, Penicillin, Ofloxacin, Norfloxacin,Ciprofloxacin Ceftriaxone, Clindamycine, Vancomycin, Oxacillin Streptomycin, Trimethoprim+ Sulfamethoxazole, Gentamicin, Apramycin, Tylosin, Colistin, Sulfamethizol, Flumequin, Oxolinic acid by disc diffusion method and ATB VET (14 289 Biomerieux) strip system. MIC values of Eritromycin antibiotic were obtained as 0.032-0.125 µg/mL in L. Garvieae strains. The values showed to sensitive of eritromycine antibiotic for all strains

Anahtar Kelimeler

Lactococcus garveiae , antimicrobial susceptibilities, minimum inhibitory concentration (MIC)

Kaynakça

• Altun S, Diler O , Adilolu AK (2004). Genotyping of Lactococcus garvieae strains from rainbow trout (Oncorhynchus mykiss) by 16S rDNA sequencing. Bull. Eur. Ass. Fish Pathol 24,119-125.
• Arda, M.,(1997) Temel Mikrobiyoloji. Medisan yayn seri No :25.Ankara.490s.
• Austin B, Van Pouce A (1993). A Gram-positive lacticacid bacterium causing steady mortalities in farmed rainbow trout, Oncorhynchus mykiss Walbaum, in the UK. Bull.Eur.Assoc.Fish Pathol. 13,114-118.
• Austin, B., Austin, D.A. (1999) Bacterial fish pathogens disease of farmed and wild fish third (Revised) Edition. Praxis Publishing Chichester, U.K., 457p.
• Bragg, R., Broero, J.S.E. (1986) Streptococcosis in rainbow trout in South Africa. Bull. Ass. Fsih Parhol. 6, 89-91
• Çargan, H and Tanrkul, T. T. (1997) A Lactococcus in a trout farm. Mediterranean Fisheries Congress. 9-11 April Izmir, Turkey, 40p.
• Carson, J., Gudkovs, N., Austin, B.,(1993) Characteristic of an Enterococcus-like bacterium from Australia and South Africa, pathogenic for rainbow trout, (Oncorhynchus mykiss) (Walbaum). Journal of Fish Dieases. 16, 381-388
• Ceschia, G., Girgetti, G., Giavenni, R., Sarti, M.(1992) A new problem for Italian trout farms: Streptococcosis in rainbow (Oncorhynchus mykiss). Bull. Eur. Ass. Fish. Pathol., 12:71-72.
• Chang, P. H., Lin, C. W. and Lee, C. (2002) Lactococcus garvieae infection of cultured rainbow trout, O. mykiss in Taiwan and associated biophysical characteristics and histopathology. Bull. Eur. Ass. Fish Pathol., 25 (5), 319- 327. Chen, S.C., Lin, Y.D., Liaw, L.L. and Wang, P.C.(2001) Lactococcus garvieae infection in the giant freshwater prawn Macrobrachium rosenbergii confirmed by polymerase cahin reaction and 16SrDNA sequencing. Dis. Aquat. Org. 45:45-52.
• Collins, C. H., Lyne, P.M. (1976) Microbiological methods. Butterworths. 521p.
• Cowan, S. T., Steel, K. J. (1970). Manual for the identification of medical bacteria. Cambridge of University Press, 216 p.
• Diler, Ö., Altun, S., Adilolu, A.K., Kubilay, A. and Ikl, B.I. (2002) First occurence of Streptococcosis affecting farmed rainbow trout (Oncorhynchus mykiss) in Turkey. Bull. Eur. Ass. Fish Pathol., 22:21-26.
• Eldar, A.C., Ghittino, C., Asanta, L., Bvozzettz, E., Goria, M. (1996) Enterococcus seriolicida is a Junior synonym of Lactococcus garvieae, a causative agent of septicemia and meningoencephalitis in Fish. Curr. Microbiol. 32:85-88.
• Eldar, A. and Ghittino, C. (1999) Lactococcus garvieae and Streptococcus iniae infections in rainbow trout (Onchorhynchus mykiss): Similar, but different diseases. Dis. Aquat. Org., 36: 227-231.
• Eldar, A., Ghittino, C., Zlotkin, A. and Bercovier, H. (1999) Biodiversity of Lactococcus garvieae isolated from fish in Europe, Asia and Australia. Appl. Environ. Microbiol. 65: 1005-1008.
• Elliott, J.A., Facklam, R.R., (1996) Antimicrobial susceptibilities of lactococcus lactis and lactococus garvieae and proposed method to discriminate between them. Journal of Clinical Microbiology,1296-1298.
• Holt, J..G., Krieg, N.R., Sneath, P:H:A. and Williams, S.T. (1994). Bergey’s mannual of determinative bacteriolgy. nigth edition. Williams&Wilkins, 787.
• Koneman, E. W., Allen, S. D., Janda, W. M., Schereekenberger, P.C., Winn, N.Y. (1997). Color atlas nad texbook of diagnostic microbiology. fifth edition, Philadelphia.
• Ksuda, R., Kawai, K., Salati, F., Banner, C.R. and Fryer, L. (1991) Enterococcus seriolicida sp. Nov., a fish pathogen. Int.J. Syst. Bacteriol., Vol. 41, 406-409pp.
• Ksuda, R. and Salati, F. (1999) Enterococcus seriolicida and Streptococcus iniae. In (eds. Woo PTK, Bruno, D.W.) Fish Diseases and Disorders, Vol: 3, Viral, Bacterial and Fungal Infections. CABI Publishing, 303-317pp.
• Kum, C., Gökbulut, C., Akar, F.,Krkan, R., Sekkin, S. (2004) Gökkua alabalklarnda (Oncorhynchus mykiss) Enterococcus seriolicida izolasyonu ve etkili antibakteriyal saaltm seçeneinin belirlenmesi. Veteriner Hekimleri Dernei Dergi..Cilt 75 Say:3,47-53.
• Mazzolini E.,Vismara D.,Ceschia G.,Malvisi J.,Fabris A., Passera A., Danielis L., Giorgetti G., (2003) In vitro activity of several chemiantibiotics against clinical isolates of fresh and marine fish pathogens.
• Muzquiz, J. L., Royo, F. M., Otega, C., Blas, I., Ruiz, I., Allonso, J. L., (1999). Pathogenicity of Streptococcosis inb rainbaw trout (Oncorhynchus mykiss) dependence on age of diseased fish Bull. Eur. Ass. Fish Pathol.,19(3), 114-119.
• NCCLS (National Committe for Clinical Laboratory Standards of Antimicrobial Susceptibility Testing) performance standards for antimicrobial susceptibility testing,eleventh information supplement. NCCLS document M100-S11 NCCLS, West Walley Road, Suite 1400, Wayne, Pennsllyvania 19087-1898.USA,2001
• Nieto, J.M. Devesa, S., Quiroga, I., Toranzo, A.E. (1995). Pathology of Enterococcus sp. Infection in farmed turbot, Scophthalmus maximus L., J. Fish Dis. 18(1), 21-30p.
• Ravelo, C., Magarinos, B., Romalde, J.l. and Toranzo, A.E. (2001) Conventional versus miniaturized systems for the phenotypic characterization of Lactococcus garvieae strains. Bull. Eur. Ass. Fish Pathol. 21:136-144.
• Salati, F., Tassi, P., Bronzi, P., (1996). Isolation of an Enterococcus-like bacterium from diseased Adriatic sturgeon, Acipenser naccarii, farmed in, Jtaly. Bull. Eur. Ass. Fish Pathol.,16(3), 96-100.
• Toranzo, A. E., Devesa, S., Heinen, P. Raza, A., Nunez, S., Barja, J.l. (1994). Streptococcosis in cultured turbot caused by Enterococcus-like bacterium. Bull. Eur. Ass. Fish Pathol., 14 (1 ),19-23.