THE RELATIONSHIP BETWEEN OXIDATIVE STRESS, NITRIC OXIDE, AND CORONARY ARTERY DISEASE

Öz

-

Anahtar Kelimeler

• -

THE RELATIONSHIP BETWEEN OXIDATIVE STRESS, NITRIC OXIDE, AND CORONARY ARTERY DISEASE

Öz

Aim: Oxidative stress and free radicals are known to have important roles in the development of atherosclerosis. Malondialdehyde (MDA), a carbonile group produced during lipid peroxidation, is used widely in determining oxidative stress. Nitric Oxide (NO) is a vasodilatator agent derived from the endothelium. The aim of our study was to investigate the relationship between MDA and NO in patient with coronary artery disease. Methods: Forty-five patients diagnosed with 50 % or more stenosis by coronary angiography were included in our study. Patients were separated as 1, 2 and 3 vessel-patients according to the number of vessels affected. Diabetics, smoking patients, patients with malignancy, renal and liver disease, and patients using nitrate preparation were excluded from the study. Fortyfive healthy individuals, who had cardiological evaluation in the last year were identified as the control group. MDA, NO, and lipid measurements were performed accordingly. Results: Serum MDA levels were significantly higher in the CAD group when compared to the controls (p0.05). No significant differences were observed in the comparison of NO levels between one, two and three vessel patients. NO levels were significantly higher in hypertensive CAD patients than the normotensive ones (p

Anahtar Kelimeler

• Coronary artery disease, malondialdehyde, nitric oxide, oxidative stress

Kaynakça

1. Holvoet P. Oxidative modification of low- density lipoproteins in atherothrombosis. Acta Cardiol 1998;53:253–60
2. Steinberg D. Low density lipoprotein oxidation significance. J Biol Chem 1997;272: 2963–6 pathobiological
3. Furchgott RF, Zawadzki JV. The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature 1980;288:373-6
4. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18:499- 502
5. Draper H, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol 1980; 186:421–31
6. Mendis S, Sobotka PA, Lejna FL, Euler DE. Breath pentane and plasma lipid peroxides in ischemic heart disease. Free Radic Biol Med 1995;19:679–84
7. Kostner K, Hornykewycz S, Yang P, et al. Is oxidative stress causally linked to unstable angina pectoris? A study in 100 CAD patients and matched controls. Cardiovasc Res 1997; 36:330–6
8. Pucheu S, Coundray C, Vanzetto G, Favier A, De Liris J. Assessment of radical activity during the acute phase of myocardial infarction following fibrinolysis: utility of assaying plasma malondialdehyde. Free Radic Biol Med 1995;19:873–81

9. Cavalca V, Cighetti G, Bamonti F, et al. Oxidative stress and homocysteine in coronary artery disease. Clin Chem 2001;47(5):887-92
10. Ribiere C, Jaubert AM, Gaudiot N, et al. White adipose tissue nitric oxide synthase: a potential source for NO production. Biochem Biophys Res Commun 1996;222:706–12
11. Elizalde M, Ryden M, van Harmelen V, et al. Expression of nitric oxide synthase in subcutaneous adipose tissue of nonobese and obese humans. J Lipid Res 2000;41: 1244–51
12. Kelm M, Feelish M, Deussen A, Strauer BE, Schrader J. Release of endothelium- derived nitric oxide in relation to pressure and flow. Cardiovasc Res 1991; 25:831–6
13. Yoon Y, Song J, Hong SH, Kim JQ. Plasma nitric oxide concentrations and nitric oxide synthase gene polymorphisms in coronary artery disease. Clin Chem 2000; 46(10):1626-30
14. Scribner AW, Loscalzo J, Napoli C. The effect of angiotensin-converting enzyme inhibition on endothelial function and oxidant stress. Eur J Pharmacol 2003;15;482(1-3):95-9
15. Napoli C, Ignarro LJ. Nitric oxide and atherosclerosis. Nitric Oxide 2001;5(2): 88-97
16. Soysal D, Savas S, Susam I, et al. The association of plasma homocysteine, coronary risk factors and serum nitrite in patients with coronary artery disease, vascular syndrome X and healthy subjects. Anadolu Kardiyol 2003; 3(1): 26-34