THE CHANGES OF ZINC, COPPER, AND IRON LEVELS IN LUNG TISSUE AFTER FORMALDEHYDE INHALATION DURING THE EARLY POSTNATAL PERIOD OF RATS

Öz

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Anahtar Kelimeler

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THE CHANGES OF ZINC, COPPER, AND IRON LEVELS IN LUNG TISSUE AFTER FORMALDEHYDE INHALATION DURING THE EARLY POSTNATAL PERIOD OF RATS

Öz

Aim: In this study, effects of inhaled formaldehyde (FA) gas, during early postnatal period on the levels of zinc, copper and iron elements and activity of total superoxide dismutase (t-SOD) enzyme in lung tissue and also the reversibility of effects of formaldehyde were examined. Methods: For this purpose newly born albino Wistar rats were exposed to 0 (control), 6 or 12 ppm FA gas for 30 days. After the treatment, rats were decapitated in 30th and 90th days. Activities of t-SOD and the levels of zinc, copper and iron were measured in lung samples. Results: A decrease in the t-SOD activity, copper and iron levels and increase in zinc levels were found in the treatment groups in comparison with control group at both 30th and 90th days measurements. Conclusion: It was thought that exposure to FA may alter the trace element levels of lung tissue including copper, zinc and iron, and induce further oxidative damage on lung tissue. Key words: Formaldehyde gas, lung, t-SOD, zinc, copper, iron

Anahtar Kelimeler

• Formaldehyde gas, lung, t-SOD, zinc, copper, iron

Kaynakça

1. Feron VJ, Till HP, de Vrijer F et al. Aldehydes: occurrence, carcinogenic potential, mechanism of action and risk assessment. Mutat Res 1991;259:363-85
2. Smith AE. Formaldehyde. Occup Med 1992;42:83-8
3. Usanmaz SE, Akarsu ES, Vural N. Neurotoxic effect of acute and subacute formaldehyde exposures in mice. Environ Toxicol Phar 2002;11:93-100
4. Stenton SC, Hendrick DJ. Formaldehyde. Immunol Allergy Clin North Am 1994;14: 635–57
5. Franklin P, Dingle P, Stick S. Raised exhaled nitric oxide in healthy children is associated with domestic formaldehyde levels. Am J Respir Crit Care Med 2000;161:1757–9
6. Freeman BA, Crapo JD. Biology of disease, free radicals and tissue injury. Lab Invest 1982;47:412-26
7. Li Y, Trus MA. Oxidative stress and its relationship to carcinogen activation. In: Cutler RG, Packer L, Bertrom J, Mori A (eds) Oxidative Stress and Aging. Birkhauser Verlag, Basel, 1995;203-20
8. Ozen OA, Yaman M, Sarsilmaz M, Songur A. Testicular zinc, copper and iron concentrations in male rats exposed to subacute and subchronic formaldehyde gas inhalation. J Trace Elem Med Bio 2002;16(2):119-22

9. Ozen OA, Songur A, Sarsilmaz M, Yaman M. Changes of Zinc, Copper, and Iron Levels in The Lung of Male Rats After Subacute (4-week) and Subchronic (13- week) Exposure to Formaldehyde. J Trace Elem Exp Med 2003;16:67-74
10. Chang JC, Gross EA, Swenberg JA, Barrow CS. Nasal cavity deposition, histopathology, and cell proliferation after single or repeated formaldehyde exposures in B6C3F1 mice and F-344 rats. Toxicol Appl Pharmacol 1983;68:161-76
11. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1998;34:497-500
12. Durak I, Yurtarslani Z, Canbolat O, Akyol O. A Methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction. Clin Chim Acta 1993;214:103-4
13. Lowry O, Rosenbraugh N, Farr L, Rondall R. Protein measurement with the Folin- phenol reagent. J Biol Chem 1951;183: 265-75 14. Heck HD, Casanova M, Starr new TB. understanding. Crit Rev Toxicol 1990;20: 397–426 toxicity:
14. Nelson N, Levine RJ, Albert RE et al. Contribution of formaldehyde to respiratory cancer. Environ Health Perspect 1986;70:23-35
15. Pazdrak K, Gorski P, Krakowiak A, Ruta U. Changes in nasal lavage fluid due to formaldehyde inhalation. Int Arch Occup Environ Health 1993;64:515–9
16. Reidel F, Hasenauer E, Barth PJ, Koziorowski A, Rieger CH. Formaldehyde exposure enhances inhalative allergic sensitization in the guinea pig. Allergy 1996;51:94–9
17. Costa M, Kraker AJ, Patierno SR. Toxicity and carcinogenicity of essential and non- essential metals. Prog Clin Biochem 1984;1:1-45
18. Goldstein S, Czapski G. Transition metal ions and oxygen radicals. Int Rev Exp Pathol 1990;31:133-64
19. Beyer W, Imlay J, Fridovich I. Superoxide dismutases. Prog Nucleic Acid Res Mol Biol 1991;40:221-53
20. Stevens RG, Kalkwarf DR. Iron, radiation, and cancer. Environ Health Perspect 1990;87:291-300
21. Toyokuni S. Iron-induced carcinogenesis: the role of redox regulation. Free Radic Biol Med 1996;20:553-66
22. Chance B, Sies H, Boveris A. Hydroperoxide metabolism in mammalian organs. Physiol Rev 1979;59:527-605
23. Klein CB, Snow ET, Frenkel K. Molecular mechanisms in metal carcinogenesis: Role of oxidative stress. In: Aruoma OI, Halliwell B (eds) Molecular Biology of Free Radicals in Human Diseases. OICA International, USA, 1998;pp 79-137
24. Prasad AS (1985) Clinical and biochemical manifestation zinc deficiency in human subjects. J Pharmacol 16:344-352
25. Taylor CG, Towner RA, Janzen EG, Bray TM. MRI detection of hyperoxia-induced lung edema in Zn-deficient rats. Free Radic Biol Med 1990;9:229-33
26. Durak I, Perk H, Kavutcu M, Canbolat O, Akyol O, Beduk Y. Adenosine deaminase, 5’ nucleotidase, xanthine oxidase, superoxide dismutase and catalase activities in cancerous and noncancerous human bladder tissues. Free Radic Biol Med 1994;16:825-31
27. Akyol O, Arslanoglu R, Durak I. Activities of free radical and DNA turn-over enzymes in cancerous and noncancerous human brain tissues. Redox Rep 1995;1: 255-9
28. Durak I, Isik AU, Canbolat O, Akyol O, Kavutcu M. Adenosine deaminase, 5’ nucleotidase, xanthine oxidase, superoxide dismutase and catalase activities in cancerous and noncancerous human laryngeal tissues. Free Radic Biol Med 1993;15:681-684
29. Nishide YNK, Otu K. Increase of superoxide dismutase activity in various leukemia. Physiol Chem Phys 1979;11: 253-6
30. Wilmer JW, Woutersen RA, Appelman LM, Leeman WR, Feron VJ. Subacute (4-week) inhalation toxicity study of formaldehyde in male rats: 8-hour intermittent versus 8- hour continuous exposures. J Appl Toxicol 1987;7:15-6
31. Woutersen RA, Appelman LM, Wilmer JW, Fakle HE, Feron VJ. Subchronic (13-week) inhalation toxicity study of formaldehyde in rats. J Appl Toxicol 1987;7:43-9
32. Martin WJ. A teratology study of inhaled formaldehyde in the rat. Reprod Toxicol 1990;4:237-9
33. Saillenfait AM, Bonnet P, de Ceaurriz J. The effects of maternally inhaled formaldehyde on embryonal and foetal development in rats. Food Chem Toxicol 1989;27:545-8