Effects Of Erdosteine On Oxidative-Antioxidative Equilibrium And On Cataract Formation In Rat Pups With Selenite-Induced Cataract

Yıl 2007, Cilt: 4 Sayı: 4, 149 - 153, 01.12.2007

Adil Kılıç Şahbettin Selek Özcan Erel

Öz

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Anahtar Kelimeler

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Effects Of Erdosteine On Oxidative-Antioxidative Equilibrium And On Cataract Formation In Rat Pups With Selenite-Induced Cataract

Öz

Aim: To investigate whether erdosteine supplementation following selenite exposure affects oxidant-antioxidant equilibrium and prevents cataract formation in rat pups. Methods: Thirty-nine Wistar-albino rat pups were divided into 3 groups. In Group 1 (n=16) only s.c saline was injected. In Group 2 (n=10) subcutaneous (s.c.) sodium selenite (30 nmol / g body weight) was injected on postpartum day 10. In Group 3 (n=13) s.c. sodium selenite (30 nmol/g body weight) were injected on postpartum day 10 and oral erdosteine (10 mg/kg body weight, daily for one week) was administered by gavage thereafter. The development of cataract was assessed weekly. The density of cataract was graded by slit-lamp biomicroscopy. On day 21, the blood was collected and lenses were removed. Oxidative stress index (OSI) and total antioxidant capacity (TAC) were determined in the lenses of the rat pups. Paraoxonase-1 (PON 1) activity was determined in the sera. Results: All of the lenses of rat pups in Group 1 remained clean. All rat pups developed dense nuclear opacity in Group 2. Eight out of 13 rat pups developed slight nuclear opacity in Group 3. Differences among the groups were statistically significant (p

Anahtar Kelimeler

Antioxidant, erdosteine, paraoxonase-1, selenite cataract

Kaynakça

• Ostadalova I, Babicky A, Obenberger J. Cataract induced by administration of a single dose of sodium selenite to suckling rats. Experientia 1978:34;222-3
• Giblin FJ, Chakrapani B, Reddy VN. Glutathione and lens epithelial function. Invest Ophthalmol Vis Sci1976:15;381- 593
• Shearer TR, Ma H, Fukiage C, Azuma M. Selenite nuclear cataract: review of the model. Mol Vis:1997:3;8
• Spector A. Oxidative stress induced cataract: mechanism of action. FASEB J 1995;9:1173-82
• Devamanoharan PS, Henein M, Morris S, et al. Prevention of selenite cataract by vitamin C. Exp Eye Res 1991;52:563-8
• Doganay S, Turkoz Y, Evereklioglu C, et al. Use of caffeic acid phenethyl ester to prevent sodium selenite-induced cataract in rats. J Cataract Refract Surg 2002;28: 1457-62
• Varma SD, Hegde KR, Effect of alpha- ketoglutarate against selenite-cataract formation. Exp Eye Res 2004:79;913-8 8. Canales A, Sanchez-Muniz FJ. Paraoxonase, something more than an enzyme? Med Clin (Barc) 2003;121:537- 48
• Aviram M, Rosenblat M, Bisgaier CL, Newton RS, Primo-Parmo SL, La Du BN. Paraoxonase inhibits high-density lipoprotein oxidation and preserves its functions: a possible preoxidative role for paraoxonase. J Clin Invest 1998;101: 1581-90
• Watson AD, Berliner JA, Hama SY, et al. Ptotective effect of high density lipoprotein Inhibition of the biological activity of minimally oxidized low density lipoprotein. J Clin Invest 1995;96:2882- 91 paraoxonase.
• Aviram M, Rosenblatt M, Billecke S, et al. Human serum paraoxonase (PON 1) is inactivated by oxidized low density lipoprotein and preserved by antioxidants. Free Radic Biol Med 1999;26:892-904
• Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005;
• Erel O. A new automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 2004;37:112-9
• Kosecik M, Erel O, Sevinc E, Selek S. Increased oxidative stress in children exposed to passive smoking. Int J Cardiol 2005;100(1):61-4
• Demirbag R, Yilmaz R, Erel O, Gultekin O, Asci D, Elbasan Z. The relationship between potency of oxidative stress and severity of dilated cardiomyopathy. Can J Cardiol 2005;21(10):851-5
• Harma M, Harma M, Erel O. Measurement of the total antioxidant response in preeclampsia with a novel automated method. Eur J Obstet Gynecol Reprod Biol 2005;118(1):47-51
• Aycicek A, Erel O, Kocyigit A. Increased oxidative stress in infants exposed to passive smoking. Eur J Pediatr 2005;164(12):775-8
• Eckerson HW, Wyte CM, La Du BN. The human serum paraoxonase / arylesterase polymorphism. Am J Hum Genet 1983;35: 1126-38
• Orhan H, Marol S, Hepşen İF, Şahin G. Effects of some probable antioxidants on selenite-induced cataract formation and oxidative stress-related parameters in rats. Toxicology 1999;139:219-32
• David LL, Shearer TR. State of sulfhydryl in selenite cataract. Toxicol Appl Pharmacol 1984;74:109-15
• Gupta SK, Joshi S. Role of naproxen as antioxidant in selenite cataract. Ophthalmic Res 1994;26:226-31
• Yagci R, Aydin B, Erdurmus M, et al. Use of melatonin to prevent selenite-induced cataract formation in rat eyes. Curr Eye Res 2006;31(10):845-50
• Gupta SK, Joshi S. Prevention of photoperoxidation of lens lipids by naproxen. Afr Asian J Ophthalmol 1993;7:215-97
• Cicek E, Sutcu R, Gokalp O, et al. The effect of isoniazid on hippocampal NMDA receptors: protective role of erdosteine. Moll Cell Biochem 2005;277(1-2):131-5
• Boyaci H, Maral H, Turan G, et al. Effects of erdosteine on bleomycin-induced lung fibrosis in rats. Moll Cell Biochem 2006;281(1-2):129-37