The Anti-Inflammatory Activity of Eugenia Caryophllata Essential Oil: An animal model of anti-inflammatory activity

Yıl 2005, Cilt: 2 Sayı: 4, 159 - 163, 01.12.2005

Abdurrahman Öztürk Hanefi Özbek

Öz

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Anahtar Kelimeler

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The Anti-Inflammatory Activity of Eugenia Caryophllata Essential Oil: An animal model of anti-inflammatory activity

Öz

Aim: The aim of this study is gas chromatographic analysis of Eugenia caryaphyllata (clove) essential oil and investigation of its anti-inflammatory effects. Methods: The study involved eight groups; Serum physiologic, ethyl alcohol, indomethacin (3 mg/kg), etodolac (50 mg/kg), cardamom (0.05 mL/kg), EC-I (0.025 mL/kg), EC-II (0.050 mL/kg), EC-III (0.100 mL/kg) and EC-IV (0.200mL/kg). After measuring the volumes of right hind-paws of rats using a plethysmometer, drugs were injected intraperitoneally and lambda-carrageenan were injected subcutaneously into the plantar region. Three hours after the injections the volume measurements of the right hind-paws were repeated and the difference between the volumes were compared. Results: The composition of the essential oil was as follows: ß-caryophyllen % 44.7, eugenol % 44.2, α-humulen % 3.5, eugenyl acetate % 1.3 and α-copaen % 1.0. It was found that indomethazin reduced the inflammation by 95.70%, etodolac by 43.42 %, EC-I by 46.55 %, EC-II by 90.15 %, EC-III by 66.94 % and EC-IV by 82.78 %. The essential oil of Eugenia caryophyllata had an anti-inflammatory effect matching to that of etodolac at 0.025 and 0.1 mL/kg and to that of indomethacin at 0.05 and 0.2 mL/kg doses. Conclusion: As a result Eugenia caryophyllata essential oil extract was shown to have an antiinflammatory effect. Key words: Eugenia caryophyllata, essential oil, anti-inflammatory activity, rats

Anahtar Kelimeler

Eugenia caryophyllata, essential oil, anti-inflammatory activity, rats

Kaynakça

• Baytop T. Therapy with Medicinal Plants in Turkey. 2nd ed. İstanbul-Turkey. Nobel Medical Bookstore 1999.
• Akgül A. Baharat Bilimi & Teknolojisi. First ed. Ankara-Turkey. Gıda Teknolojisi Derneği Yayınları Nr:15:1993
• Yang YC, Lee SH, Lee WJ, Choi DH, Ahn YJ. Ovicidal and adulticidal effects of Eugenia caryophyllata bud and leaf oil compounds on Pediculus capitis. J Agric Food Chem 2003;51(17):4884-8
• Burt SA, Reinders RD. Antibacterial activity of selected plant essential oils against Escherichia coli O157:H7. Lett Appl Microbiol 2003;36(3):162-7
• Ernst E. The Desktop Guide to Complementary and Alternative Medicine. China. Mosby 2001
• Bae EA, Han MJ, Kim NJ, Kim DH. Anti- Helicobacter pylori activity of herbal medicines. Biol Pharm Bull 1998;21(9): 990-2
• Kim EH, Kim HK, Ahn YJ. Acaricidal activity of clove bud oil compounds against Dermatophagoides farinae and Dermatophagoides pteronyssinus (Acari: Pyroglyphidae). J Agric Food Chem 2003;51(4):885-9
• Hong CH, Hur SK, Oh OJ, Kim SS, Nam KA, Lee SK. Evaluation of natural products on inhibition of inducible cyclooxygenase (COX-2) and nitric oxide synthase (iNOS) in cultured mouse macrophage cells. J Ethnopharmacol 2002;83(1-2):153-9
• Kim HJ, Lee JS, Woo ER et al. Isolation of virus-cell fusion inhibitory components from Eugenia caryophyllata. Planta Med 2001;67(3):277-9
• Pourgholami MH, Kamalinejad M, Javadi M, Majzoob S, Sayyah M. Evaluation of the anticonvulsant activity of the essential oil of Eugenia caryophyllata in male mice. J Ethnopharmacol 1999;64(2):167-71 11. Zheng LK. (Eugenia caryophyllata) as potential anticarcinogenic agents. J Nat Prod 1992;55(7):999-1003 PM, Lam clove
• Sesquiterpenes from
• Özbek H, Öztürk M, Öztürk A, Ceylan E, Yener Z. Determination of lethal doses of volatile and fixed oils of several plants. Eastern Journal of Medicine 2004;9(1): 4-6
• Özgülen H. İksir-i Şifa. 2nd ed. İstanbul- Turkey. Timaş Press 1998
• Yalçın A. Prescriptions of Herbal Medicine. İstanbul-Turkey. Geçit Press 1999
• Pamuk HA. Şifalı Bitkiler ve Emraz (Herbal Medicine of Turkey). İstanbul- Turkey. Pamuk Press 1995.
• Pamuk HA. Şifalı Bitkiler Ansiklopedisi (Encyclopedia of Herbal Medicine). İstanbul-Turkey. Pamuk Press 1998.
• Winter CA Risley EA, Nuss GW. Carrageenin-induced edema in hind paw of the rats as an assay for antiinflammatory drugs. Proc Soc Exp Biol Med 1962;111: 544-7
• Rimbau V, Cerdan C, Vila R. Antiinflammatory activity of some extracts from plants used in the traditional medicine of North-African countries (II). Phytother Res 1999;13:128-32
• Inoue K, Motonaga A, Nishimura T et al. Mechanism of anti-inflammatory action of etodolac. Arzneimittelforschung 1991;41(3):235-9
• Kouadio F, Kanko C, Juge M. Analgesic and antiinflammatory activities of an extract from Parkia biglobosa used in traditional medicine in the Ivory Coast. Pytother Res 2000;14:635-7
• Kim SS, Oh OJ, Min HY et al. Eugenol suppresses cyclooxygenase-2 expression in lipopolysaccharide-stimulated mouse macrophage RAW264.7 cells. Life Sci 2003;73(3):337-48
• Sharma JN, Srivastava KC, Gan EK. Suppressive effects of eugenol and ginger oil on arthritic rats. Pharmacol 1994;49(5):314-8