Changes in the levels of MDA and GSH in mice

Abstract

Abstract. Aluminum (Al) is widely distributed in the environment and enters the human body by air, water, food and drugs. It is claimed that Al toxicity increases the rate of lipid peroxidation and hence the formation of free radicals in some investigations. As an index of lipid peroxidation, serum and tissue malondialdehyde (MDA) levels increase, whereas there is a decrease in the anti- oxidant glutathione (GSH). It has been observed that the increase in the levels of MDA recovers with the administration of vitamin E. twenty four adult mice were divided into three groups; Al administered, Al+vitamin E and controls. 300 mg/kg body weight of Al sulfate were given to Al and Al+vitamin E groups for three months orally. 20 mg/kg body weight of vitamin E was additionally given to Al+vitamin E group subcutaneously once a week during this period of time. Liver and spleen tissue as well as serum samples were obtained. MDA and GSH levels of the samples were analyzed. We found statistically significant increase in MDA levels of both serum and tissue samples while there was a decrease in the GSH levels. We also observed a recovery on these changes caused by chronic Al administration with vitamin E addition. Chronic high dose of Al sulfate can lead to tissue oxidative injury, and Vitamin E is capable of preventing the deleterious effects of Al⁺³ ions.

Key words: Aluminum, lipid peroxidation, serum, liver, spleen

Keywords

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Kaynakça

1. Wang M, Ruan D, Chen J, Xu Y: Lack of effects of vitamin E on aluminium-induced deficit of synaptic plasticity in rat dentate gyrus in vivo. Food Chem Toxicol 2002;40: 471-478.
2. Lukiw WJ, Mclachlan DR: Neurotoxicology of aluminium. In Effects and Mechanisms, Handbook of Neurotoxicology (Edited by Chang L, Dyer R), Marcel Dekker, New York, 2002;70-80.
3. Farina M, Lara FS, Brandao R, Jacques R, Rocha JBT: Effects of aluminum sulfate on erythropoiesis in rats. Toxicol Lett 2002;132: 131-139.
4. Kim MS, Lenore S, Clesceri LS: Aluminum exposure: a study of an effect on cellular growth rate. Sci Total Environ 2001;278: 127-135.
5. Levesque L, Mizzen CA, McLachlan DR, Fraser PE: Ligand specific effects on aluminium incorporation and toxicity in neurons and astrocytes. Brain Res 2000;877: 191-202.
6. Nayak P: Aluminum: Impacts and disease. Environ Res 2002;89:101-115.
7. Roberts NB, Zhu H, Kim JY, Shin HR, Kim JI, Choi SY: Further studies on the interrelationship of aluminum and silicon in patients receiving aluminum hydroxide therapy for dyspepsia and factors that relate to the solubilization of aluminum. J Trace Elem Exp Med 2002;15: 9-19.
8. Dlugaszek M, Fiejka MA, Graczyk A, Aleksandrowwicz JC, Slowikowska M: Effects of various aluminium compounds given orally to mice on Al tissue distribution and tissue concentrations of essential elements. Pharmacol Toxicol 2000;86: 135-139.

9. Schetinger MR, Bonan CD, Morsch VM, Bohrer D, Valentim LM, Rodrigues SR: Effects of aluminum sulfate on delta- aminolevulinate dehydratase from kidney, brain, and liver of adult mice. Braz J Med Biol Res 1999;32: 761-766.
10. Athar M, Abdulla H, Sultana S, Favier A, Pero R: Free radicals and trace elements. J Trace Elem Exp Med 1993;6: 65-73.
11. Frei B: Molecular and biological mechanisms of antioxidant action FASEB 13: 963-964, 1999.
12. Barry H: Antioxidants and human disease. A general introduction. Nutr Rev 1997;55: 544-549.
13. Halliwell B: Free radicals, antioxidants and human disease: curiosity, cause or consequence? The Lancet 1994;344: 721-724.
14. Floyd RA: Role of oxygen free radicals in carcinogenesis and brain ischemia. FASEB 1990;4: 2587-2597.
15. Halliwell B, Gutteridge J: Lipid peroxidation, oxygen radicals, cell damage, and antioxidant therapy. Lancet 1984;23: 1396-1398.
16. Ohkawa H, Ohishi N: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95: 351- 358.
17. Singh V: A current perspective on nutrition and exercise. J Nutr 122: 760-765, 1992.
18. Witt E, Reznick A, Viguie C, Starke R, Packer L: Exercise, oxidative damage and effects of antioxidant manipulation. J Nutr 1992;122: 766- 773.
19. Armstrong RN: Structure, catalytic mechanism, and evolution of the glutathione transferases. Chem Res Toxicol 1997;10: 2-18.
20. Van Bladeren PJ: Glutathione conjugation as a bioactivation reaction. Chem Biol Interact 2000;129: 61–76.
21. Hsu CH, Chi BC, Liu MY, Li JH, Chen CJ Chen RY: Phosphine-induced oxidative damage in rats: role of glutathione.Toxicology 2002;179: 1-8.
22. Burton GW, Joyce A, Ingold KU: First proof that vitamin E is major lipid-soluble, chain-breaking antioxidant in human blood plasma. Lancet 1982;2: 327-329.
23. Horvath ME, Faux SP, Smith AG, Blazovics A, Looij M, Feher J, Cheeseman KH: Vitamin E protects against iron- hexachlorobenzene induced porphyria and formation of 8- hydroxydeoxyguanosin in the liver of C57BL/10SCSn mice. Toxicol Lett 122: 97-102, 2001.
24. Parker L: Protective role of vitamin E in biological systems. Am J Clin Nutr 1991;53: 1050S-1055S.
25. Horvath ME, Blazovics A, Kemeny T, Vasarhelyi B, Weinbrenner Z, Feher J: The effect of vitamin E on experimental hyperlipidemia. Orv Hetil 1993;134: 1757-1760.
26. Omera FO, Blakley BR: Vitamin E is protective against iron toxicity and iron-induced hepatic vitamin E depletion in mice. J Nutr 1993;123: 1649-1655.
27. Latchoumycandane C, Chitra KC, Mathur PP: The effect of methoxychlor on the epididymal antioxidant system of adult rats. Reprod Toxicol 2002;16: 161–172.
28. Erol U, Gurdal M, Erol A, Aslan R, Konukoğlu D, Onmus H: Is midazolam effective as an antioxidant in preventing reperfusion injury in rat kidney? Int Urol Nephrol 2002;34: 121- 127.
29. Moron MS, Depierre JW, Mannervik B: Level of glutathione, glutathione reductase and glutathione- S-transferase activity in rat lung and liver. Biochem Biophys Acta 1979;82: 67-78.
30. Deloncle R, Huguet F, Babin P, Fernandez B, Quellard N, Guillard O: Chronic administration of aliminum L-glutamate in young mature rats-effects on iron levels and lipid- peroxidation in selected brain areas. Toxicol Lett 1999;104: 65-73,.
31. Chugh SN, Arora V, Sharma A, Chugh K: Free radical scavengers and lipid peroxidation in acute aluminium phosphide poising. Indian J Med Res 1996;104: 190-193.
32. Fraga CG, Oteiza PI, Golub MS, Gershwin ME, Keen CL: Effects of aluminum on brain lipid peroxidation. Toxicol Lett 1990;51: 213-219.
33. Zatta P, Kiss T, Suwalsky M, Berthon G: Aluminium (III) as a promoter of cellular oxidation. Coordin Chem Rev 2002;228: 271-284.
34. Guo CH, Huang CJ, Chiou YL, Hsu GSW: Alteration of trace element distribution and testis ACE activity in mice with high peritoneal aluminum. Biol Trace Elem Res 2002;86: 145-157.
35. Julka D, Gill KD: Effect of aluminium on regional brain antioxidant defense status in Wistar rats. Res Exp Med 1996;196: 187-194.
36. Swain C, Chainy GBN: Effects of aluminium sulfate and citric acid ingestion on lipid peroxidation and on activities of superoxide dismutase and catalase in cerebral hemisphere and liver of developing young chicks. Mol Cell Biochem 1998;187: 163-172.
37. Swain C, Chainy GB: Aluminum effect on lipid peroxidation and on the activities of superoxide dismutase and catalase in the cerebral hemisphere and liver of young chicks. J Trace Elem Med Biol 1997;11: 77-82.
38. Chugh SN, Kolley T, Kakkar R, Chugh K, Sharma A: A critical evaluation of antiperoxidant effect of intravenous magnesium in acute aluminium phosphide poisoning. Magnes Res 1997;10: 225- 230.
39. Hsu C, Han B, Liu M, Yeh C, Casida JE: Phosphine- induced oxidative damage in rats: attenuation by melatonin. Free Radic Biol Med 2000;28: 636-642.
40. Rahman I, MacNee W: Lung glutathione and oxidative stress: implications in cigarette smoke- induced airway disease. Am J Physiol 1999;277:: L1067–L1068.
41. Kelly FJ: Glutathione: in defence of the lung. Food Chem Toxicol 1999;37: 963–966.
42. Sokol RJ, Taylor SF, Devereaux MW, Khandwala R, Sondheimer NJ, Shikes RH, Mierau G: Hepatic oxidant injury and glutathione depletion during total parenteral nutrition in weanling rats. Am J Physiol 1996;270: G691–G700.
43. Wang X, Kanel GC, DeLeve LD: Support of sinusoidal endothelial cell glutathione prevents hepatic veno-occlusive disease in the rat. Hepatology 2000;31: 428–434.
44. Bouchard G, Yousef IM, Barriault C, Tuchweber B: Role of glutathione and oxidative stress in phalloidin-induced cholestasis. J Hepatol 2000;32: 550–560.
45. Scholz RW: Mechanism of interaction of vitamin E and glutathione in the protection against lipid peroxidation. Ann N Y Acad Sci 1989;570: 514– 517.
46. Scholz RW, Reddy PW, Wynn MK, Graham KS, Liken AD, Gumpricht E, Reddy CC: Glutathione- dependent factors and inhibition of rat liver microsomal lipid peroxidation. Free Radic Biol Med 1997;23: 815–828.
47. Julka D, Gill KD: Effects of aluminium on regional brain antioxidant defense status in wistar rats. Res Exp Med 1996;196: 187-194.
48. Broquist HP: Buthionine sulfoximine an experimental tool to induce glutathione deficiency: elucidation of glutathione and ascorbate in their role as antioxidants. Nutr Rev 1991;50: 110–111.
49. Fattah AA, Yousef HM, Bekairi AM, Sawaf HA: Vitamin E protects the brain against oxidative injury stimulated by excessive aluminium intake. Biochem Mol Biol Int 1998;46: 1175-1180.