Contribution of influenza viruses, human metapneumovirus and respiratory syncytial virus to acute respiratory infections in children in northern Greece, 2008 – 2010

Yıl 2012, Cilt: 17 Sayı: 1, 24 - 29, 24.01.2013

E. Chatzopoulou A. Melidou G. Gioula M. Exindari D. Chatzidimitriou F. Chatzopoulou N. Malisiovas

https://izlik.org/JA55TJ69UL

Öz

Abstract. Influenza viruses, respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) are the most common pathogens that cause acute respiratory disease in children. The aim of this study is to present the contribution of the above three pathogens to influenza-like illness (ILI) in children (aged <6 years old) during 2-year (2008-2010) influenza seasons in N. Greece. 430 pharyngeal swabs from children younger than 6 years, presented as ILI infections during the last two influenza seasons (2008-2009 and 2009-2010) were examined for influenza A and B, RSV and hMPV, by one step Real-time RT-PCR. Influenza viruses were detected in 122 (28.3%) of the 430 spesimens, RSV in 45 (10.4%) samples and hMPV in 28 (6.5%). RSV and influenza viruses' co-infections were observed in eight cases, RSV and hMPV co-infections in four cases and hMPV with influenza viruses was found in one case. The majority of the patients (67.7%) were between 3 and 6 years old. Our results demonstrate that influenza viruses, RSV and hMPV contribute to ILI presenting infections at a rate of 45.2% in children younger than 6 years old.

Key words: influenza virus, human metapneumovirus (hMPV), respiratory syncytial virus (RSV), Northern Greece                                                                                                                                               

Anahtar Kelimeler

influenza virus , human metapneumovirus (hMPV) , respiratory syncytial virus (RSV) , Northern Greece

Kaynakça

• 1. Esper F, Boucher D, Weibel C, Martinello R, Kahn JS. Human metapneumovirus infection in the United States: clinical manifestations associated with a newly emerging respiratory infection in children. Pediatrics 2003; 111: 1407-1410.
• 2. www.who.int/topics/influenza/en/
• 3. van den Hoogen BG, de Jong JC, Groen J, et al. A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med 2001; 7: 719-724.
• 4. Papapanagiotou J, Kyriazopoulou-Dalaina V. Medical Microbiology and virology, second edition, Thessalonica. University studio press 2004.
• 5. Nair H, Nokes DJ, Gessner BD, et al. Global burden of acute lower respiratory infections due to respiratory syncytial virus in young children: a systematic review and meta-analysis. Lancet 2010; 375: 1545-1555.
• 6. Melidou A, Exindari M, Gioula G, et al. Molecular and phylogenetic analysis and vaccine strain match of human influenza A(H3N2) viruses isolated in Northern Greece between 2004 and 2008. Virus Res 2009; 145: 220-226.
• 7. CDC, 2009a Centre of Disease Control, CDC Realtime RTPCR Protocol for Detection and Characterization of Swine Influenza (Version 2009) (2009) CDC ref: #I-007-05.
• 8. Bonroy C, Vankeeerberghen A, Boel A, De Beenhouwer H. Use of a multiplex real-time PCR to study the incidence of human metapneumovirus and human respiratory syncytial virus infections during two winter seasons in a Belgian paediatric hospital. Clin Microbiol Infect 2007; 13: 504-509.
• 9. Gioula G, Chatzidimitriou D, Melidou A, Exindari M, Kyriazopoulou-Dalaina V. Contribution of human metapneumovirus to influenza-like infections in North Greece, 2005-2008. Euro Surveill 2010; 15: 19499.
• 10. Lovato-Salas F, Matienzo-Serment L, MonjarasAvila C, et al. Pandemic influenza A(H1N1) 2009 and respiratory syncytial virus associated hospitalizations. J Infect 2010; 61: 382-390.
• 11. Foulongne V, Guyon G, Rodiere M, Segondy M. Human metapneumovirus infection in young children hospitalized with respiratory track disease. Pediatr Infect Dis J 2006; 25: 354-359.
• 12. Wolf DG, Greenberg D, Kalkstein D, et al. Comparison of human metapneumovirus, respiratory syncytial virus and influenza A virus lower respiratory tract infections in hospitalized young children. Pediatr Infect Dis J 2006; 25: 320-324.
• 13. Dollner H, Risnes K, Radtke A, Nordbo SA. Outbreak of human metapneumovirus infection in Norwegian children. Pediatr Infect Dis J 2004; 23: 436-440.
• 14. Berkley JA, Munywoki P, Ngama M, et al. Viral etiology of severe pneumonia among Kenyan infants and children. JAMA 2010; 303: 2051-2057.
• 15. Xepapadaki P, Psarras St, Bossios A, et al. Human metapneumovirus as a causative agent of acute bronchiolitis in infants. J Clin Virol 2004; 30: 267- 270.
• 16. Chen X, Zhang ZY, Zhao Y, Liu EM, Zhao XD. Acute lower respiratory tract infections by human metapneumovirus in children in Southwest China: A 2-year study. Pediatr Pulmonol 2010; 45: 824-831.