Immunohistochemical Demonstration of Intermediate Trophoblasts for Differential Diagnosis of Uterine and Ectopic Pregnancy

Abstract

Objective: The objective in the study was to detect the
intermediate trophoblastic cells and to differentiate the
abortion from ectopic pregnancy by using
immunohistochemical methods.
Method: Four groups of patients were included in the
study, and the archival materials of the patients were
used for immunohistochemistry. Group 1 comprised
of 10 patients who had miscarriages between the 5th
and 12th weeks of gestation. There were 5 non pregnant
patients in group 2. In group 3, there were 64 patients
who had ectopic pregnancy between the 5th and 12th
weeks of gestation. Group 4 comprised of 50 patients
who were clinically suspected of miscarriage without
chorionic villi, and cyto and syncytiotrophoblast.
Results: All trophoblastic cells of chorionic villi showed
strong positivity for sitokeratin in group 1 though this
was not the condition in group 2 and 3. In group 4,
however, 35 of 50 patients had intermediate trophoblast
cells as detected by cytokeratin staining.
Conclusion: The use of these hormone markers in
endometrial specimens increases precision in the
diagnosis of intrauterine versus ectopic pregnancy.
Key words: Trophoblast, immunohistochemistry, ectopic
pregnancy

Keywords

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