Ultrastructural and immunohistochemical evaluation of endometrial tissues of infertile women with recurrent implantation failure

Yıl 2013, Cilt: 18 Sayı: 1, 1 - 7, 02.05.2013

Leyla Bahar , Semra Kahraman Bora Reşitoglu Tülin Baykal

https://izlik.org/JA47UF55FB

Öz

Abstract. To evaluateendometrial tissues of fertile women and the women with recurrent implantationfailure (RIF) analyzed by using transmissionelectron microscopy (TEM) and MUC1 immunohistochemistry.  In this study, 23 patients with RIF and 11 fertile women participated. Endometrial tissuesamples were examined by light and electron microscopes. Additionaly, MUC1immunoreactivity was evaluated. Electron microscopic examination of theendometrial surface epithelial cells of the recurrent implantation failurerevealed lots of cytoplasmic extensions containing secretory vacuoles andirregular microvilli. Furthermore, most of the endometrial cells exhibitedsecretory vacuoles in the supranuclear region. Immunoreactivity of MUC1 wasdetected weakly in the luminal epithelium of the control group whereas thestaining intensity was conspicuous in RIFgroup. Significant differences between the groups with respect to glandularepithelium were found. Endometrial tissues of both fertile women and women withRIF were thought to be important in regulatingthe stage for embryo implantation. Further studies should be done to have abetter understanding of factors involved in the establishment of endometrialreceptivity.Key words: Endometrial tissue, endometrialreceptivity, recurrent implantation failure, electron microscopy, MUC1

Anahtar Kelimeler

Endometrial tissue , endometrial receptivity , recurrent implantation failure , electron microscopy , MUC1

Kaynakça

• Attar E. Spermatogenezis, Fertilizasyon, Erken embriyo gelişimi ve İmplantasyon. In: Çolgar U, (eds). Reproductif Endokrinoloji ve İnfertilite (1st.ed). Istanbul: Istanbul Medical, 2006, pp. 21-27.
• Paria BC, Lim H, Wang XN, et al. Coordination of different effects of primary estrogen and catecholestrogen on two distinct targets mediates embryo implantation in the mouse. Endocrinology 1998; 139: 5235-5246.
• Susan JK. Molecular Interactions at the MaternalEmbryonic Interface During the Early Phase of Implantation. Sem Reproductive Med 2000; 18: 2372
• Sunder S, Lenton E. Endocrinology of the PeriImplantation Period. Clin Obstet Gynecol 2000; 14: 789-800.
• Bahar L. The luteal phase endometrium of fertile women and women with recurrent implantation failure Autologous Endometrial Tissue and Tissue Kokültür-Level Comparison of Fine Structure. Phd. Theses. University of Mersin. 2008. Aydın Publishing, Mersin.
• Tan BK, Vandekerckhove P, Kennedy R, Keay SD. Investigation and current management of recurrent IVF treatment failure in the UK. BJOG 2005; 112: 773-7
• El-Toukhy T, Taranissi M. Towards better quality research in recurrent implantation failure: standardizing its definition is the first step. RBM Online 2006; 12: 383-385.
• İltemur DC, Şatıroğlu H, Berker B, Çetinkaya H, Kahraman K. Yardımla üreme tekniklerinde implantasyon ve gebelik oranlarını etkileyen faktörler. Turkiye Klinikleri J Gynecol Obst 2003; 13: 466-475.
• Ledee-Bataille N, Lapree-Delage G, Taupin JL. Concentration of leukaemia inhibitory factor (LIF) in uterine flushing fluid is highly predictive of embryo implantation. Hum Reprod 2002; 17: 2132
• Rashid NA, Lalitkumar S, Lalitkumar PG, GemzellDanielsson K. Endometrial receptivity and human embryo implantation. Am J Reprod Immunol 2011; 66: 23-30.
• Dey SK, Das SK, Reese J, et al. Molecular cues to implantation. Endocr Rev 2004; 25: 341-373.
• Gipson IK, Ho SB, Spurr-Michaud SJ, et al. Mucin genes expressed by human female reproductive tract epithelia. Biol Reprod 1997; 56: 999-1011.
• Achache H, Revel A. Endometrial receptivity markers, the journey to successful embryo implantation. Hum Reprod Update 2006; 12: 7317
• Lee CN, Chang SW, Cho NH, Cho S. Nitrous oxide synthase expression in placenta of preeclampsia. J Korean Med Sci 1997; 12: 532-538.
• Mutlu C, Koyutürk M, Karpuz V. Evaluation of endothelial nitric oxide synthase (eNOS) immunreactivity of fetal and maternal placenta. Cerrahpaşa J Med 2005; 36: 109-115.
• Akar M , Kurşun S , Taşkın Ö. Tekrarlayan in vitro fertilizasyon başarısızlıklarında yaklaşım. Türk Fertil Der 2004; 12: 217-224.
• Tan BK, Vandekerckhove P, Kennedy R, Keay SD. Investigation and current management of recurrent IVF treatment failure in the UK. BJOG 2005; 112: 773-7
• Gökçimen A, Temel S. İmplantasyon ve moleküler etkileşimler. S.D.Ü. Tıp Fak Derg 2004; 11: 25-33.
• Duc-Goiran P, Mignot TM, Bourgeois C, Ferre F. Embryo- Maternal Interactions at the Implantation Site: A Delicate Equilibrium. Eur J Obstet Gynecol Reprod Biol 1999; 83: 85-100.
• Chervenak JL, Ilsley NP. Episialin acts as an antiadhesive factor in an in vitro model of human endometrial-blastocyst mattachment. Biol Reprod 2000; 63: 294-300.
• Meseguer M, Pellicer A, Simon C. MUC1 and endometrial receptivity. Mol Hum Reprod 1998; 4: 1089-1098.
• Campbell S, Swann HR, Aplin JD, et al. CD44 is expressed throughout pre-implantation human embryo development. Hum Reprod 1995; 10: 4254
• Tabibzadeh S. Molecular control of the implantation window. Hum Reprod Update 1998; 4: 465-471.
• Bahar L, Baykal T. The role of endometrial receptivity on the implantation. MEÜ. Sağlık Bilimleri Dergisi 2008; 1: 1-6.
• Serle E, Aplin JD, Tin-chiu L, et al. Endometrial differentiation in the peri-implantation phase of women with recurrent miscarriage: Amorphological and immunohistochemical study. Fertil Steril 1994; 62: 989-996.
• Hey NA, Li TC, Devine PL, et al. MUC1 in secretory phase endometrium: expression in precisely dated biopsies and flushings from normal and recurrent miscarriage patients. Hum Reprod 1995; 10: 2655-2662.
• Aplin JD. MUC-1 glycosylation in endometrium: possible roles of the apical glycocalyx at implantation. Human Reprod 1999; 14: 17-25.
• Hey NA, Aplin JD. Sialyl-Lewis x and Sialyl-Lewis a are associated with MUC1 in human endometrium. Glycoconj J 1996; 13: 769-779.
• Aplin JD, Hey NA, Graham RA. Human endometrial MUC1 carries keratan sulfate: characteristic glycoforms in the luminal epithelium at receptivity. Glycobiology 1998; 8: 269-276.
• Hey NA, Graham RA, Seif MW, Aplin JD. The polymorphic epithelial mucin MUC1 in human endometrium is regulated with maximal expression in the implantation phase. J Clinic Endocrinol Metab 1994; 78: 337-342.
• DeLoia JA, Krasnow JS, Brekosky J, et al. Regional specialization of the cell membrane-associated, polymorphic mucin (MUC1) in human uterine epithelia. Hum Reprod 1998; 13: 2902-2909.
• Horne AW, White JO, Lalani el-N. Adhesion molecules and the normal endometrium. BJOG 2002; 109: 610-617.
• Meseguer M, Aplin JD, Caballero-Campo P, O Connor JE, Martin JC. Human endometrial mucin MUC1 is up-regulated by progesterone and downregulated in vitro by the human blastocyst. Biol Reprod 2001; 64: 590-601.
• Thathiah A, Brayman M, Dharmaraj N, et al. Tumor necrosis factor alpha stimulates MUC1 synthesis and ectodomain release in a human uterine epithelial cell line. Endocrinology 2004; 145: 4192-4203.
• Horne AW, White JO, Margara RA, et al. MUC1: a genetic susceptibility to infertility? Lancet 2001; 357: 1336-1337.