BibTex RIS Kaynak Göster
Yıl 2012, Cilt: 17 Sayı: 1, 11 - 16, 24.01.2013

Öz

Kaynakça

  • Al-Bayati FA, Al-Mola HF. Antibacterial and antifungal activities of different parts of Tribulus terrestris L. growing in Iraq. J Zhejiang Univ Sci B 2008; 9: 154-159.
  • Aliero AA, Afolayan AJ. Antimicrobial activity of Solanum tomentosum. African Journal of Biotechnology 2005; 5: 369-372.
  • Ayo RG, Amupitan JO, Zhao Y. Cytotoxicity and anti-microbial studies of 1,6,8-trihydroxy-3-methyl- anthraquinone (Emodin) isolated from the leaves of Cassia nigricans Vahl. African Journal of Biotechnology 2007; 6: 1276-1279.
  • Biradar YS, Jagatap S, Khandelwal KR, Singhania SS. Exploring of Antimicrobial Activity of Triphala Mashi-an Ayurvedic Formulation. Evid Based Complement Alternat Med 2008; 5: 107-113.
  • Bastos ML, Lima MR, Conserva LM, et al. Studies on the antimicrobial activity and brine shrimp toxicity of Zeyheria tuberculosa (Vell.) Bur. (Bignoniaceae) extracts and their main constituents. Ann Clin Microbiol Antimicrob 2009; 8: 16.
  • CLSI Methods for determining bactericidal activity of antimicrobial agents. Tentative standards M26-T. National Committee for Clinical Laboratory standards, Wayne, 2007.
  • Collins CH, Lyne PM, Grange JM. Microbiological methods. Butterworth-Heinemann, Great Britain, 7th edition, pp. 1995; 178-205.
  • Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12: 564-582.
  • Doughart JH, Okafor B. Anti-microbial activity of Senna alata linn. East and Central Africa Journal of Pharmaceutical sciences 2007; 10: 17-21.
  • Drew WL, Barry AL, O'Toole R, Sherris JC. Reliability of the Kirby-Bauer disc diffusion method for detecting methicillin-resistant strains of Staphylococcus aureus. Appl Microbiol 1972; 24: 240-247.
  • Gurib-Fakim A, Schmelzer G. Medicinal plants in Africa. PROTA, pp. 6. 2007.
  • Houghton PJ, Raman A. Laboratory Hand book for the Fractionation of Natural Extracts. Chapman and Hall, London, 1st edition, pp. 1-153.1998.
  • Huie CW. A review of modern sample-preparation techniques for the extraction and analysis of medicinal plants. Anal Bioanal Chem 2002; 373: 23-30.
  • Kaur GJ, Arora DS. Antibacterial and phytochemical screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi. BMC Complement Altern Med 2009; 9: 30.
  • Kokwaro JO. Medicinal plants of East Africa. East Africa literature bureau, Nairobi Kenya, pp.149. 1993.
  • Matasyoh JC, Kiplimo JJ, Karubiu NM, Hailstorks TP. Chemical composition and antimicrobial activity of essential oil of Tarchonanthus camphorates. Journal of Food Chemistry 2007; 101: 1183-1187.
  • Mbaveng AT, Ngameni B, Kuete V, et al. Antimicrobial activity of the crude extracts and five flavonoids from the twigs of Dorstenia barteri (Moraceae). J Ethnopharmacol 2008; 116: 483-489.
  • Mbwambo ZH, Moshi MJ, Masimba PJ, Kapingu MC, Nondo RS. Antimicrobial activity and brine shrimp toxicity of extracts of Terminalia brownii roots and stem. BMC Complement Altern Med 2007; 7: 9.
  • Mohamed LT, El Nur BS, Abdelrahman MN. The Antibacterial, antiviral activities and phytochemical screening of some Sudanese medicinal plants. EurAsian Journal of BioSciences 2010; 4: 8-16.
  • Moshi MJ, Mbwambo ZH, Kapingu MC, et al. Antimicrobial and Brine Shrimp Lethality of Extracts of Terminalia Mollis Laws. African Journal of Traditional, Complimentary and alternative Medicine 2006; 3: 59-69.
  • Mothana RA, Abdo S.A, Hasson S, Althawab FM. Antimicrobial, Antioxidant and Cytotoxic Activities and Phytochemical Screening of Some Yemeni Medicinal Plants. Journal of Evidence-based Complimentary and alternative medicine, doi:10.1093/ecam/nen004: Also available from; http://ecam.oxfordjournals.org. 2008.
  • Navarro MC, Montilla MP, Cabo MM, et al. Antibacterial, antiprotozoal and antioxidant activity of five plants used in Izabal for infectious diseases. Phytother Res 2003; 17: 325-329.
  • Ngoci SN, Mwendia CM, Mwaniki CG. Phytochemical and cytotoxicity testing of Indigofera lupatana Baker F. Journal of Animal & Plant Sciences 2011; 11: 1364-1373. Available from http://www.biosciences.elewa.org/JAPS
  • Nguemeving JR, Azebaze AG, Kuete V, et al. Laurentixanthones A and B, antimicrobial xanthones from Vismia laurentii. Phytochemistry 2006; 67: 1341-1346.
  • Ogunwenmo KO, Idowu OA, Innocent C, Esan EB Oyelana OA. Cultivars of Codiaeum variegatum (L.) Blume (Euphorbiaceae) show variability in phytochemical and cytological characteristics. Journal of. Biotechnology 2007; 20: 2400-2405.
  • Okeke MI, Iroegbu CU, Eze EN, Okoli AS, Esimone CO. Evaluation of extracts of the root of Landolphia owerrience for antibacterial activity. J Ethnopharmacol 2001; 78: 119-127.
  • Okuda T. Systematics and health effects of chemically distinct tannins in medicinal plants. Journal of Phytochemistry 2005, 66: 2012-2031.
  • Olila D, Olwa-Odyek, Opuda-Asibo J. Antibacterial and antifungal activities of extracts of Zanthoxylum chalybeum and Warburgia ugandensis, Ugandan medicinal plants. Afr Health Sci 2001; 1: 66-72.
  • Parekh J, Chanda S. In-vitro antimicrobial activities of extractsof Launaea procumbens Roxb. (Labiateae), Vitis vinifera L. (Vitaceae) and Cyperus rotundus L. (Cyperaceae). African Journal of Biomedical Research 2006; 9: 89-93.
  • Riley BW, Brokensha D. (1988). The Mbeere in Kenya; Botanical identity and use Vol (ii), University press of America, USA, pp. 1988; 76-77.
  • Roberts SC. Production and engineering of terpenoids in plant cell culture. Nat Chem Biol 2007; 3: 387-395.
  • Samy RP, Gopalakrishnakone P. Review: Therapeutic potential of plants as anti-microbials for drug discovery. Journal of Evidence-Based Complimentary and Alternative medicine:doi:10.1093/ecam/nen036. 2008.
  • Soares MB, Brustolim D, Santos LA, et al. Physalins B, F and G, seco-steroids purified from Physalis angulata L., inhibit lymphocyte function and allogeneic transplant rejection. Int Immunopharmacol 2006; 6: 408-414.
  • Tomczykowa M, Tomczyk M, Jakoniuk P, Tryniszewska E. Antimicrobial and antifungal activities of the extracts and essential oils of Bidens tripartita. Folia Histochem Cytobiol 2008; 46: 389- 393.
  • Trombetta D, Castelli F, Sarpietro MG, et al. Mechanisms of antibacterial action of three monoterpenes. Antimicrob Agents Chemother 2005; 49: 2474-2478.
  • Victor J, Siebert S, Hoare D, Wyk BV. (2005). Sekhukhuneland grasslands: A treasure house of biodiversity. Available at; http://www.fao.org/ag/AGP/agpc/doc/show/SAfrica/ sapaper/saessay.htm-accessed on 17/06/2010
  • Wojcikowski K, Wohlmuth H, Johnson DW, Rolfe M, Gobe G. An in vitro investigation of herbs traditionally used for kidney and urinary system disorders: potential therapeutic and toxic effects. Nephrology (Carlton) 2009; 14: 70-79.
  • Wojcikowski K, Stevenson L, Leach D, Wohlmuth H, Gobe G. Antioxidant capacity of 55 medicinal herbs traditionally used to treat the urinary system: a comparison using a sequential three-solvent extraction process. J Altern Complement Med 2007; 13:103-109.

Antibacterial activity of methanol root extract of Indigofera lupatana Baker F.

Yıl 2012, Cilt: 17 Sayı: 1, 11 - 16, 24.01.2013

Öz

Abstract. Indigofera lupatana Baker F. (locally known as Mugiti) has been used by Mbeere community of Kenya to treat cough, diarrhea, pleurisy and gonorrhea. These infectious diseases are caused by pathogenic micro-organisms such as, Klebsiella pneumoniae, Salmonella typhi, Escherichia coli, and Neisseria gonorrhea, among others. Infectious diseases are a cause of morbidity and mortality in humans and animals. Their effects are further aggravated by drug resistance, making it difficult to contain these infections. This calls for search of new drugs that will mitigate these problems. Indigenous plants are promising as a cheap alternative source of new therapeutic agents. Powdered sample of I. lupatana Baker F. roots were extracted using methanol solvent. The resultant extract was subjected to anti-microbial assay. The extract showed the highest activity against Bacillus subtilis (28.0 mm), Bacillus cereus (22.0 mm), Escherichia coli (21.7mm), Staphylococcus aureus (16.7 mm), Klebsiella pneumonia (15.3 mm) and Proteus mirabilis (12.3 mm), Pseudomonas aeruginosa (11.7 mm), Salmonella typhimurium (11.3 mm). The phytochemical studies of extract fractions showed presence of phenolics, flavonoids, tannins, saponins, terpenoids, cardiac glycosides, steroids and phlobatannins. These compounds are responsible for the bioactivity of the sample fractions. The activity was greater among the Gram positive bacteria than Gram negative bacteria. The MIC ranged from between 25 to 400mg/ml.

Key words: Indigofera lupatana Baker F., Antimicrobial activity, phytochemical, MIC

Kaynakça

  • Al-Bayati FA, Al-Mola HF. Antibacterial and antifungal activities of different parts of Tribulus terrestris L. growing in Iraq. J Zhejiang Univ Sci B 2008; 9: 154-159.
  • Aliero AA, Afolayan AJ. Antimicrobial activity of Solanum tomentosum. African Journal of Biotechnology 2005; 5: 369-372.
  • Ayo RG, Amupitan JO, Zhao Y. Cytotoxicity and anti-microbial studies of 1,6,8-trihydroxy-3-methyl- anthraquinone (Emodin) isolated from the leaves of Cassia nigricans Vahl. African Journal of Biotechnology 2007; 6: 1276-1279.
  • Biradar YS, Jagatap S, Khandelwal KR, Singhania SS. Exploring of Antimicrobial Activity of Triphala Mashi-an Ayurvedic Formulation. Evid Based Complement Alternat Med 2008; 5: 107-113.
  • Bastos ML, Lima MR, Conserva LM, et al. Studies on the antimicrobial activity and brine shrimp toxicity of Zeyheria tuberculosa (Vell.) Bur. (Bignoniaceae) extracts and their main constituents. Ann Clin Microbiol Antimicrob 2009; 8: 16.
  • CLSI Methods for determining bactericidal activity of antimicrobial agents. Tentative standards M26-T. National Committee for Clinical Laboratory standards, Wayne, 2007.
  • Collins CH, Lyne PM, Grange JM. Microbiological methods. Butterworth-Heinemann, Great Britain, 7th edition, pp. 1995; 178-205.
  • Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12: 564-582.
  • Doughart JH, Okafor B. Anti-microbial activity of Senna alata linn. East and Central Africa Journal of Pharmaceutical sciences 2007; 10: 17-21.
  • Drew WL, Barry AL, O'Toole R, Sherris JC. Reliability of the Kirby-Bauer disc diffusion method for detecting methicillin-resistant strains of Staphylococcus aureus. Appl Microbiol 1972; 24: 240-247.
  • Gurib-Fakim A, Schmelzer G. Medicinal plants in Africa. PROTA, pp. 6. 2007.
  • Houghton PJ, Raman A. Laboratory Hand book for the Fractionation of Natural Extracts. Chapman and Hall, London, 1st edition, pp. 1-153.1998.
  • Huie CW. A review of modern sample-preparation techniques for the extraction and analysis of medicinal plants. Anal Bioanal Chem 2002; 373: 23-30.
  • Kaur GJ, Arora DS. Antibacterial and phytochemical screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi. BMC Complement Altern Med 2009; 9: 30.
  • Kokwaro JO. Medicinal plants of East Africa. East Africa literature bureau, Nairobi Kenya, pp.149. 1993.
  • Matasyoh JC, Kiplimo JJ, Karubiu NM, Hailstorks TP. Chemical composition and antimicrobial activity of essential oil of Tarchonanthus camphorates. Journal of Food Chemistry 2007; 101: 1183-1187.
  • Mbaveng AT, Ngameni B, Kuete V, et al. Antimicrobial activity of the crude extracts and five flavonoids from the twigs of Dorstenia barteri (Moraceae). J Ethnopharmacol 2008; 116: 483-489.
  • Mbwambo ZH, Moshi MJ, Masimba PJ, Kapingu MC, Nondo RS. Antimicrobial activity and brine shrimp toxicity of extracts of Terminalia brownii roots and stem. BMC Complement Altern Med 2007; 7: 9.
  • Mohamed LT, El Nur BS, Abdelrahman MN. The Antibacterial, antiviral activities and phytochemical screening of some Sudanese medicinal plants. EurAsian Journal of BioSciences 2010; 4: 8-16.
  • Moshi MJ, Mbwambo ZH, Kapingu MC, et al. Antimicrobial and Brine Shrimp Lethality of Extracts of Terminalia Mollis Laws. African Journal of Traditional, Complimentary and alternative Medicine 2006; 3: 59-69.
  • Mothana RA, Abdo S.A, Hasson S, Althawab FM. Antimicrobial, Antioxidant and Cytotoxic Activities and Phytochemical Screening of Some Yemeni Medicinal Plants. Journal of Evidence-based Complimentary and alternative medicine, doi:10.1093/ecam/nen004: Also available from; http://ecam.oxfordjournals.org. 2008.
  • Navarro MC, Montilla MP, Cabo MM, et al. Antibacterial, antiprotozoal and antioxidant activity of five plants used in Izabal for infectious diseases. Phytother Res 2003; 17: 325-329.
  • Ngoci SN, Mwendia CM, Mwaniki CG. Phytochemical and cytotoxicity testing of Indigofera lupatana Baker F. Journal of Animal & Plant Sciences 2011; 11: 1364-1373. Available from http://www.biosciences.elewa.org/JAPS
  • Nguemeving JR, Azebaze AG, Kuete V, et al. Laurentixanthones A and B, antimicrobial xanthones from Vismia laurentii. Phytochemistry 2006; 67: 1341-1346.
  • Ogunwenmo KO, Idowu OA, Innocent C, Esan EB Oyelana OA. Cultivars of Codiaeum variegatum (L.) Blume (Euphorbiaceae) show variability in phytochemical and cytological characteristics. Journal of. Biotechnology 2007; 20: 2400-2405.
  • Okeke MI, Iroegbu CU, Eze EN, Okoli AS, Esimone CO. Evaluation of extracts of the root of Landolphia owerrience for antibacterial activity. J Ethnopharmacol 2001; 78: 119-127.
  • Okuda T. Systematics and health effects of chemically distinct tannins in medicinal plants. Journal of Phytochemistry 2005, 66: 2012-2031.
  • Olila D, Olwa-Odyek, Opuda-Asibo J. Antibacterial and antifungal activities of extracts of Zanthoxylum chalybeum and Warburgia ugandensis, Ugandan medicinal plants. Afr Health Sci 2001; 1: 66-72.
  • Parekh J, Chanda S. In-vitro antimicrobial activities of extractsof Launaea procumbens Roxb. (Labiateae), Vitis vinifera L. (Vitaceae) and Cyperus rotundus L. (Cyperaceae). African Journal of Biomedical Research 2006; 9: 89-93.
  • Riley BW, Brokensha D. (1988). The Mbeere in Kenya; Botanical identity and use Vol (ii), University press of America, USA, pp. 1988; 76-77.
  • Roberts SC. Production and engineering of terpenoids in plant cell culture. Nat Chem Biol 2007; 3: 387-395.
  • Samy RP, Gopalakrishnakone P. Review: Therapeutic potential of plants as anti-microbials for drug discovery. Journal of Evidence-Based Complimentary and Alternative medicine:doi:10.1093/ecam/nen036. 2008.
  • Soares MB, Brustolim D, Santos LA, et al. Physalins B, F and G, seco-steroids purified from Physalis angulata L., inhibit lymphocyte function and allogeneic transplant rejection. Int Immunopharmacol 2006; 6: 408-414.
  • Tomczykowa M, Tomczyk M, Jakoniuk P, Tryniszewska E. Antimicrobial and antifungal activities of the extracts and essential oils of Bidens tripartita. Folia Histochem Cytobiol 2008; 46: 389- 393.
  • Trombetta D, Castelli F, Sarpietro MG, et al. Mechanisms of antibacterial action of three monoterpenes. Antimicrob Agents Chemother 2005; 49: 2474-2478.
  • Victor J, Siebert S, Hoare D, Wyk BV. (2005). Sekhukhuneland grasslands: A treasure house of biodiversity. Available at; http://www.fao.org/ag/AGP/agpc/doc/show/SAfrica/ sapaper/saessay.htm-accessed on 17/06/2010
  • Wojcikowski K, Wohlmuth H, Johnson DW, Rolfe M, Gobe G. An in vitro investigation of herbs traditionally used for kidney and urinary system disorders: potential therapeutic and toxic effects. Nephrology (Carlton) 2009; 14: 70-79.
  • Wojcikowski K, Stevenson L, Leach D, Wohlmuth H, Gobe G. Antioxidant capacity of 55 medicinal herbs traditionally used to treat the urinary system: a comparison using a sequential three-solvent extraction process. J Altern Complement Med 2007; 13:103-109.
Toplam 38 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Articles
Yazarlar

Sospeter Njerua Ngoci Bu kişi benim

Josphat C. Matasyoh Bu kişi benim

Charles Gathinji Mwaniki Bu kişi benim

Charles Maina Mwendia Bu kişi benim

Yayımlanma Tarihi 24 Ocak 2013
Yayımlandığı Sayı Yıl 2012 Cilt: 17 Sayı: 1

Kaynak Göster

APA Ngoci, S. N., Matasyoh, J. C., Mwaniki, C. G., Mwendia, C. M. (2013). Antibacterial activity of methanol root extract of Indigofera lupatana Baker F. EASTERN JOURNAL OF MEDICINE, 17(1), 11-16.
AMA Ngoci SN, Matasyoh JC, Mwaniki CG, Mwendia CM. Antibacterial activity of methanol root extract of Indigofera lupatana Baker F. EASTERN JOURNAL OF MEDICINE. Mart 2013;17(1):11-16.
Chicago Ngoci, Sospeter Njerua, Josphat C. Matasyoh, Charles Gathinji Mwaniki, ve Charles Maina Mwendia. “Antibacterial Activity of Methanol Root Extract of Indigofera Lupatana Baker F”. EASTERN JOURNAL OF MEDICINE 17, sy. 1 (Mart 2013): 11-16.
EndNote Ngoci SN, Matasyoh JC, Mwaniki CG, Mwendia CM (01 Mart 2013) Antibacterial activity of methanol root extract of Indigofera lupatana Baker F. EASTERN JOURNAL OF MEDICINE 17 1 11–16.
IEEE S. N. Ngoci, J. C. Matasyoh, C. G. Mwaniki, ve C. M. Mwendia, “Antibacterial activity of methanol root extract of Indigofera lupatana Baker F”., EASTERN JOURNAL OF MEDICINE, c. 17, sy. 1, ss. 11–16, 2013.
ISNAD Ngoci, Sospeter Njerua vd. “Antibacterial Activity of Methanol Root Extract of Indigofera Lupatana Baker F”. EASTERN JOURNAL OF MEDICINE 17/1 (Mart 2013), 11-16.
JAMA Ngoci SN, Matasyoh JC, Mwaniki CG, Mwendia CM. Antibacterial activity of methanol root extract of Indigofera lupatana Baker F. EASTERN JOURNAL OF MEDICINE. 2013;17:11–16.
MLA Ngoci, Sospeter Njerua vd. “Antibacterial Activity of Methanol Root Extract of Indigofera Lupatana Baker F”. EASTERN JOURNAL OF MEDICINE, c. 17, sy. 1, 2013, ss. 11-16.
Vancouver Ngoci SN, Matasyoh JC, Mwaniki CG, Mwendia CM. Antibacterial activity of methanol root extract of Indigofera lupatana Baker F. EASTERN JOURNAL OF MEDICINE. 2013;17(1):11-6.