Genome-Wide Association Mapping Using a Bayesian Mixture Model for Plant Height in Oryza sativa

Yıl 2016, Cilt: 2 Sayı: 2, 41 - 46, 30.07.2016

Burak Karacaören

Öz

Genotypic and phenotypic data could be used to predict inheritance of complex traits for plant breeding in genome wide
association mapping studies (GWAS). In GWAS using a single marker model may leads to suboptimal use of genotypic
datasets. Alternatively, using whole genome, a Bayesian mixture model may cluster markers into predefined classes. We
used 413 diverse accessions of Oryza sativa with 36900 Single Nucleotide Polymorphisms (SNPs) markers for plant
height. We assumed different genetic architectures for the phenotype. We estimated genotypic heritability as 0.61. Bayesian
mixture model detected 144, 446, 54 SNPs with explanatory levels of 0.0001, 0.001 and 0.01 respectively. Chromosome
1 (n=109), and 3 (n=85) had the highest explanatory genetic variances as 23% and 19%, respectively. Correlation between
genomic predicted observations and actual observations was found to be 0.94. Since GWAS are mostly based on only one
replication as was also the case in this study; results need to be confirmed by independent validation experiments.

Anahtar Kelimeler

Genome wide association mapping studies, Bayesian mixture model, Single Nucleotide Polymorphisms Markers

Kaynakça

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