Sürekli Dalga NIR-IR Dalgaboylarının Karaciğerde Oluşturduğu Isıl Etkilerin in vitro Ortamda Araştırılması / Investigation of Photothermal Effects of CW NIR-IR Lasers on Liver Tissue in vitro

Yıl 2012, Cilt: 2 Sayı: 4, 63 - 67, 23.10.2012

Fikret Yıldız , Ayşe Sarp Çağlar Gök Murat Gülsoy İnci Çilesiz

Öz

Bu çalışmasında, NIR-IR (1070 nm, 980 nm ve 1940 nm) dalgaboylu üç farklı laser, karaciğer dokusuna 1 cm mesafeden uygulanarak, oluşan ışılısıl zararın (koagülasyon, ısıl yanık ve ablasyon) zamansal ve boyutsal (çap ve derinlik) tespiti yapılmıştır. Ölçülen değerler, istatistiksel fark (p<0,05) göz önüne alınarak karşılaştırmalı olarak değerlendirilmiştir. Dokuda oluşan koagülasyon çap ve derinliği önce makroskopik olarak (kalipiyer ile) daha sonra da histolojik olarak (H&E boyaması ile) ölçülerek karşılaştırılmıştır. Ölçüm sonuçlarına göre, 1940 nm dalgaboyu, doku tarafından soğurulması daha fazla olduğu için en hızlı etkileşime girip doku hasarı oluşturan dalgaboyu olmuştur. Birim zamanda en büyük doku hasarı yine bu dalgaboyunda oluşmuştur. 980 nm dalgaboyu laser demet çapı ve uygulama süresinin daha büyük olması nedeniyle hacimsel hasar bu dalgaboyunda en yüksek değerlerdedir.

Anahtar Kelimeler

1070 nm, 980 nm, 1940nm, karaciğer dokusu, (H&E)boyaması

Investigation of Photothermal Effects of CW NIR-IR Lasers on Liver Tissue in vitro

Öz

In this experimental study, three different laser sources in the NIR-IR region, 980 nm, 1070 nm, and 1940 nm were applied from 1 cm distance on liver tissue. Photothermal damage (coagulation, carbonization and ablation) was assessed as a function of exposure time, and damage dimensions (diameter and depth) were measured. Results were statistically (p<0.005) analyzed and histological evaluations were performed using H&E staining. Measurements and histology were compared and contrasted. Our observations showed that 1940 nm interacts with liver tissue quickest due to relatively higher absorption coefficient at this wavelength. Largest tissue damage occurred at 1940 nm. Damage volumes were highest at 980 nm than at 1070 nm.

Anahtar Kelimeler

1070nm, 980nm, 1940nm, liver tissue, (H&E)staining

Kaynakça

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