N-asetil sisteinin sinonazal siliyer atım frekansına etkileri

Öz

Amaç: N-asetil sisteinin (NAC) insan respiratuvar epitelyal hücre kültüründe, başta elektrolit transportu ve siliyer atım frekansı olmak üzere farmakolojik etkilerinin değerlendirilmesi.

Yöntem: Hava-sıvı arayüzünde üretilmiş, iyi diferansiye edilmiş insan bronşiyal epitelyal hücre kültüründe farklı konsantrasyonlarda NAC uygulaması yapıldı. İdeal siliyer atım frekansını sağlayan NAC konsantrasyonu saptandı. N-asetil sisteinin atım hızına etkisi saptandıktan sonra, etkinliği ATP veya IBMX ile araştırılarak etki mekanizması ortaya konmaya calışıldı. Siliyer atım frekansı Sissons-Ammons video analiz sistemi ile saptandı.

Bulgular: Siliyer fonksiyonlarda maksimum stimulasyonun 10 mg/ml NAC konsantrasyonunda olduğu saptandı. Yıkama sonrası siliyer hareketler dramatik olarak arttı. Bu artış NAC artı IBMX ve NAC artı ATP yıkamalarından sonra daha da artış gösterdi.

Sonuç: N-asetil sisteinin apikal kullanımı, siliyer atım frekansını belirgin düzeyde artırmaktadır. N-asetil sistein sonrası PBS ile yıkamak ile klinik etkinlik elde edilebilir.

Anahtar sözcükler: N-asetil sistein, sinonazal, siliya.

Anahtar Kelimeler

• N-asetil sistein, sinonazal, siliya

N-acetylcysteine effects on sinonasal cilia function

Abstract

Objective: To evaluate the pharmacological effects of the N-acetylcysteine (NAC) on human respiratory epithelial cultures specifically addressing electrolyte transport and cilia beat frequency. Methods: Well-differentiated human bronchial epithelial cultures grown at an air liquid interface were treated on the apical or basolateral surface with varying concentrations of NAC. The best NAC concentration for ideal cilia beat frequency was found. The effects of NAC were evaluated on cilia beat frequency. After the effect of N-acetylcysteine on beat rate was found, its efficiency was investigated by ATP or IBMX to understand its mechanism of action. Changes in ciliary beat frequency were determined using the Sissons-Ammons video analysis system. Results: Maximal stimulatory effect on cilia function was evident at 10 mg/ml NAC concentration. After wash up, cilia movement were increased very dramatically. This increase of cilia beat frequency was even higher after NAC plus IBMX and NAC plus ATP washings. Conclusion: Apical application of NAC prominently stimulates cilia beat frequency and after wash up, cilia movement was increased very dramatically. After NAC use by washing with PBS in clinical efficacy can be enhanced

Keywords

• N-acetylcysteine,sinonasal,cilia

Kaynakça

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22. Please cite this article as: Kapusuz Gencer Z, Saydam L, Cohen NA, Cingi C. N-acetylcysteine effects on sinonasal cilia function. ENT Updates 2015; 5(3):87–92.