N-asetil sisteinin sinonazal siliyer atım frekansına etkileri

Yıl 2015, Cilt: 5 Sayı: 3, 87 - 92, 14.01.2016

Zeliha Kapusuz Gencer , Levent Saydam Noam A. Cohen Cemal Cingi

Öz

Amaç: N-asetil sisteinin (NAC) insan respiratuvar epitelyal hücre kültüründe, başta elektrolit transportu ve siliyer atım frekansı olmak üzere farmakolojik etkilerinin değerlendirilmesi.

Yöntem: Hava-sıvı arayüzünde üretilmiş, iyi diferansiye edilmiş insan bronşiyal epitelyal hücre kültüründe farklı konsantrasyonlarda NAC uygulaması yapıldı. İdeal siliyer atım frekansını sağlayan NAC konsantrasyonu saptandı. N-asetil sisteinin atım hızına etkisi saptandıktan sonra, etkinliği ATP veya IBMX ile araştırılarak etki mekanizması ortaya konmaya calışıldı. Siliyer atım frekansı Sissons-Ammons video analiz sistemi ile saptandı.

Bulgular: Siliyer fonksiyonlarda maksimum stimulasyonun 10 mg/ml NAC konsantrasyonunda olduğu saptandı. Yıkama sonrası siliyer hareketler dramatik olarak arttı. Bu artış NAC artı IBMX ve NAC artı ATP yıkamalarından sonra daha da artış gösterdi.

Sonuç: N-asetil sisteinin apikal kullanımı, siliyer atım frekansını belirgin düzeyde artırmaktadır. N-asetil sistein sonrası PBS ile yıkamak ile klinik etkinlik elde edilebilir.

Anahtar sözcükler: N-asetil sistein, sinonazal, siliya.

Anahtar Kelimeler

N-asetil sistein, sinonazal, siliya

N-acetylcysteine effects on sinonasal cilia function

Öz

Objective: To evaluate the pharmacological effects of the N-acetylcysteine (NAC) on human respiratory epithelial cultures specifically
addressing electrolyte transport and cilia beat frequency.
Methods: Well-differentiated human bronchial epithelial cultures
grown at an air liquid interface were treated on the apical or basolateral
surface with varying concentrations of NAC. The best NAC concentration for ideal cilia beat frequency was found. The effects of NAC were
evaluated on cilia beat frequency. After the effect of N-acetylcysteine on
beat rate was found, its efficiency was investigated by ATP or IBMX to
understand its mechanism of action. Changes in ciliary beat frequency
were determined using the Sissons-Ammons video analysis system.
Results: Maximal stimulatory effect on cilia function was evident at 10
mg/ml NAC concentration. After wash up, cilia movement were
increased very dramatically. This increase of cilia beat frequency was
even higher after NAC plus IBMX and NAC plus ATP washings.
Conclusion: Apical application of NAC prominently stimulates cilia
beat frequency and after wash up, cilia movement was increased very
dramatically. After NAC use by washing with PBS in clinical efficacy
can be enhanced

Anahtar Kelimeler

N-acetylcysteine, sinonasal, cilia

Kaynakça

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• Please cite this article as: Kapusuz Gencer Z, Saydam L, Cohen NA, Cingi C. N-acetylcysteine effects on sinonasal cilia function. ENT Updates 2015; 5(3):87–92.