Glutathione peroxidase and catalase enzyme gene polymorphisms in profound congenital hearing loss

Yıl 2017, Cilt: 7 Sayı: 3, 126 - 130, 28.11.2017

Orhan Tunç Elif Baysal Sibel Oğuzkan Balcı Semih Mumbuç Nihal Güngör Tunç Sacide Pehlivan Muzaffer Kanlıkama

Öz

Objective: The aim of this study was to
search the codon 200 polymorphism on the glutathione peroxidase 1 gene
(GPX1) and A/T changes on the promoter region of the catalase gene (CAT)
in cochlear implant patients with congenital profound hearing loss.




Methods:
Sixty-five cochlear implant patients with congenital hearing loss and
100 age- and gender-matched healthy volunteers were evaluated between
2011 and 2013. Genomic DNA was extracted from peripheral blood samples
by using the salting out procedure. The T/A polymorphism in the promoter
region of the CAT gene (rs7943316) and GPX1 gene codon 200 proline to
leucine substitution (rs1050450) were determined by polymerase chain
reaction and restriction fragment length polymorphisms.




Results: No
statistically significant difference was found in CC and CT genotypes in
codon 200 on GPX1 (CC, p=0.10; CT, p=0.48) However, there was a
statistically significant difference in the TT genotype (p=0.04). In the
CAT promoter region, there was no statistically significant difference
between the patients and control groups (AA, p=0.41; TA, p=0.16; TT,
p=0.08).




Conclusion: As a
conclusion, the TT genotype on the GPX1 codon 200 may have a
relationship with congenital profound sensorineural hearing loss.

Anahtar Kelimeler

Catalase, cochlear implant, congenital sensorineural hearing loss, glutathione peroxidase, polymorphism

Kaynakça

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