Detection of human papilloma virus in normal and tumoral oropharyngeal tissue using HPV DNA in situ hybridization and p16 expression and its clinicopathologic importance

Yıl 2018, Cilt: 8 Sayı: 1, 13 - 18, 05.04.2018

Özlem Önerci Çelebi Ebru Şener Makbule Çisel Aydın Gaye Güler Tezel

Öz

Objective: The rise in the number of cancer
cases with human papilloma virus (HPV)-positive squamous carcinoma of
the oropharynx makes the detection of HPV clinically important. We aimed
to investigate the HPV positivity in our patients who have
oropharyngeal cancer and compare the two different HPV detection
methods, which are HPV in situ hybridization (ISH) and p16
immunohistochemistry (IHC), and show the staining patterns.




Methods:
Twenty-three specimens of oropharyngeal cancer patients and ten
tonsillectomy specimens that revealed no cancerous tissue (control
group) were collected from retrospective file analysis. All specimens
were evaluated by both p16 IHC and HPV ISH on paraffin blocks.




Results:
Seven of 23 cases showed p16 expression. Of all these 7 cases that
showed p16 expression, six showed high p16 expression and one showed low
p16 expression. All six cases that showed high p16 expression were HPV
ISH (+). One case that showed low expression of p16 was HPV ISH (-). All
cases that were p16 (+) showed diffuse p16 expression and none of the
cases showed focal p16 expression.




Conclusion: High
p16 expression (>70%) is a reliable marker of HPV positivity.
Combining p16 IHC with HPV ISH will further improve its specificity. All
p16 positive cases showed diffuse p16 expression, thus did not show
tumor heterogeneity, suggesting that even a biopsy specimen showing
diffuse p16 expression shows p16 positivity of the whole tumoral tissue.

Anahtar Kelimeler

Human papilloma virus, oropharyngeal cancer, p16 immunohistochemistry, in situ hybridization

Kaynakça

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