Yıl 2019, Cilt 9 , Sayı 2, Sayfalar 159 - 165 2019-08-08

Inter-retrotransposon polymorphism polymerase chain reaction as a tool for screening HERV polymorphisms in nasal mucosal swabs

Ahmet Hamdi Kepekçi [1] , Merve Seda İbişoğlu [2] , Sibel Yılmaz [3] , Cenk Kığ [4]


Objective: Inter-retrotransposon polymorphism Polymerase Chain Reaction (IRAP-PCR) technique allows for detecting insertional polymorphisms via amplification of the DNA fragment between two retrotransposons in plant genomes. However, this method has not been reported to be used for analyzing human samples to date. Recently, Human Endogenous Retrovirus (HERV) polymorphisms gained interest due to their potential effect on pathophysiology of certain diseases. Nevertheless, the association between HERV polymorphisms and the risk for developing nasal polyposis (NP) has not been studied. In this study, we aimed to investigate whether or not IRAP-PCR could be performed in nasal swab samples for comparing HERV polymorphisms in different nasal mucosal samples.

Methods: Nasal swab samples from 16 patients were used for DNA isolation. These DNA samples were used as templates for IRAP PCR of HERV-K6, HERV-K11, HERV-L1 and HERV-L2 and PCR products were analyzed by agarose gel electrophoresis.

Results: Nasal swab samples yielded enough DNA material for successfully performing IRAP-PCR. We obtained specific banding patterns the three out of four HERV sequences tested in this study. No polymorphisms was detected between samples from different patients. Similarly, polymorphic bands was not detected between the polyps or nasal mucosal swab samples obtained from the same patient.

Conclusion: We have, for the first time, shown that IRAPPCR can be performed in nasal swabs. Our findings suggest that this technique can serve as an inexpensive and effective screening tool for investigating links between nasal mucosal diseases and HERV polymorphisms such as nasal polyposis.

Human endogenous retrovirus, genetic polymorphism, polymerase chain reaction, nasal polyps
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Orcid: 0000-0002-5332-5234
Yazar: Ahmet Hamdi Kepekçi (Sorumlu Yazar)
Kurum: Istanbul Yeni Yuzyil University, Vocational Health High School, Department of Audiometry, Istanbul/TURKEY
Ülke: Turkey


Orcid: 0000-0001-9237-4357
Yazar: Merve Seda İbişoğlu
Kurum: Istanbul Yeni Yuzyil University, Faculty of Arts and Sciences, Molecular Biology and Genetics Department

Orcid: 0000-0002-9930-3567
Yazar: Sibel Yılmaz
Kurum: Istanbul Yeni Yuzyil University, Faculty of Arts and Sciences, Molecular Biology and Genetics Department
Ülke: Turkey


Orcid: 0000-0002-0450-5175
Yazar: Cenk Kığ (Sorumlu Yazar)
Kurum: ISTANBUL YENI YUZYIL UNIVERSITY, FACULTY OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES (MEDICINE), DEPARTMENT OF MEDICAL BIOLOGY AND GENETICS
Ülke: Turkey


Tarihler

Başvuru Tarihi : 17 Haziran 2019
Kabul Tarihi : 24 Temmuz 2019
Yayımlanma Tarihi : 8 Ağustos 2019

Bibtex @araştırma makalesi { entupdates578602, journal = {ENT Updates}, issn = {2149-7109}, eissn = {2149-6498}, address = {}, publisher = {Sürekli Eğitim ve Bilimsel Araştırmalar Derneği}, year = {2019}, volume = {9}, pages = {159 - 165}, doi = {10.32448/entupdates.578602}, title = {Inter-retrotransposon polymorphism polymerase chain reaction as a tool for screening HERV polymorphisms in nasal mucosal swabs}, key = {cite}, author = {Kepekçi, Ahmet Hamdi and İbişoğlu, Merve Seda and Yılmaz, Sibel and Kığ, Cenk} }
APA Kepekçi, A , İbişoğlu, M , Yılmaz, S , Kığ, C . (2019). Inter-retrotransposon polymorphism polymerase chain reaction as a tool for screening HERV polymorphisms in nasal mucosal swabs. ENT Updates , 9 (2) , 159-165 . DOI: 10.32448/entupdates.578602
MLA Kepekçi, A , İbişoğlu, M , Yılmaz, S , Kığ, C . "Inter-retrotransposon polymorphism polymerase chain reaction as a tool for screening HERV polymorphisms in nasal mucosal swabs". ENT Updates 9 (2019 ): 159-165 <https://dergipark.org.tr/tr/pub/entupdates/issue/47766/578602>
Chicago Kepekçi, A , İbişoğlu, M , Yılmaz, S , Kığ, C . "Inter-retrotransposon polymorphism polymerase chain reaction as a tool for screening HERV polymorphisms in nasal mucosal swabs". ENT Updates 9 (2019 ): 159-165
RIS TY - JOUR T1 - Inter-retrotransposon polymorphism polymerase chain reaction as a tool for screening HERV polymorphisms in nasal mucosal swabs AU - Ahmet Hamdi Kepekçi , Merve Seda İbişoğlu , Sibel Yılmaz , Cenk Kığ Y1 - 2019 PY - 2019 N1 - doi: 10.32448/entupdates.578602 DO - 10.32448/entupdates.578602 T2 - ENT Updates JF - Journal JO - JOR SP - 159 EP - 165 VL - 9 IS - 2 SN - 2149-7109-2149-6498 M3 - doi: 10.32448/entupdates.578602 UR - https://doi.org/10.32448/entupdates.578602 Y2 - 2019 ER -
EndNote %0 ENT Updates Inter-retrotransposon polymorphism polymerase chain reaction as a tool for screening HERV polymorphisms in nasal mucosal swabs %A Ahmet Hamdi Kepekçi , Merve Seda İbişoğlu , Sibel Yılmaz , Cenk Kığ %T Inter-retrotransposon polymorphism polymerase chain reaction as a tool for screening HERV polymorphisms in nasal mucosal swabs %D 2019 %J ENT Updates %P 2149-7109-2149-6498 %V 9 %N 2 %R doi: 10.32448/entupdates.578602 %U 10.32448/entupdates.578602
ISNAD Kepekçi, Ahmet Hamdi , İbişoğlu, Merve Seda , Yılmaz, Sibel , Kığ, Cenk . "Inter-retrotransposon polymorphism polymerase chain reaction as a tool for screening HERV polymorphisms in nasal mucosal swabs". ENT Updates 9 / 2 (Ağustos 2019): 159-165 . https://doi.org/10.32448/entupdates.578602
AMA Kepekçi A , İbişoğlu M , Yılmaz S , Kığ C . Inter-retrotransposon polymorphism polymerase chain reaction as a tool for screening HERV polymorphisms in nasal mucosal swabs. ENT Updates. 2019; 9(2): 159-165.
Vancouver Kepekçi A , İbişoğlu M , Yılmaz S , Kığ C . Inter-retrotransposon polymorphism polymerase chain reaction as a tool for screening HERV polymorphisms in nasal mucosal swabs. ENT Updates. 2019; 9(2): 165-159.