An investigation of 8-hydroxy-2’-deoxyguanosine and 8-iso-prostaglandin F2α levels in patients with larynx carcinoma

Yıl 2020, Cilt: 10 Sayı: 2, 335 - 339, 25.08.2020

Yaser Said Cetin , Ufuk Düzenli Mehmet Berköz , Hüseyin Özkan Nazım Bozan

https://doi.org/10.32448/entupdates.744725

Öz

Objectives: 8-hydroxy-2’-deoxyguanosine (8-OHdG) and 8-iso-prostaglandin F2α (8-iso-PGF2α) are indicators of oxidative stress in the human body. The aim of the present study was to investigate serum 8-OHdG and 8-iso-PGF2α levels in patients with larynx carcinoma and to compare them with healthy controls.

Methods: A total of 50 patients, consisting of 25 patients with larynx carcinoma (study group) and 25 healthy subjects (control group), were enrolled in the present study. Serum 8-OHdG concentration was measured using an ELISA kit, whereas an enzyme immunoassay kit was utilized for the measurement of serum 8-iso-PGF2α levels. TNM stages of the patients were recorded from the patient records in the study group.

Results: 8-OHdG and 8-iso-PGF2α levels were significantly higher in patients with larynx carcinoma than the control subjects. 8-OHdG levels were significantly higher in patients with LN metastases than the patients without LN metastases in the study group.

Conclusion: The findings of the present study showed the presence of significantly increased serum 8-OHdG and 8-iso-PGF2α levels in patients with larynx carcinoma, which may support the role of oxidative stress in the development laryngeal cancers. 

Anahtar Kelimeler

Larynx cancer, oxidative stress, 8-hydroxy-2′-deoxyguanosine, prostaglandin F2 alpha

Kaynakça

• 1. Zhang SS, Xia QM, Zheng RS, Chen WQ. Laryngeal cancer incidence and mortality in China, 2010. J Cancer Res Ther 2015;11:143-8.
• 2. Steuer CE, El-Deiry M, Parks JR, Higgins KA, Saba NF. An update on larynx cancer. CA Cancer J Clin 2017;67:31-50.
• 3. Sies H. Oxidative stress: a concept in redox biology and medicine. Redox Biol 2015;4:180-3.
• 4. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 2010;4:118-26. 5. Sies H, Stahl W, Sundquist AR. Antioxidant functions of vitamins. Vitamins E and C, beta-carotene, and other carotenoids. Ann N Y Acad Sci 1992;669:7-20.
• 6. Siddartha SB, Kumari S, Rathan S, Babu S, Shetty S. Status of serum sialic acid, antioxidant and lipid peroxidation in head and neck cancer patients. Res J Pharma Biol Chem Sci 2011;2:1-5.
• 7. Nagai MA. Genetic alterations in head and neck squamous cell carcinomas. Braz J Med Biol Res 1999;32:897-904.
• 8. Mazlumoglu MR, Ozkan O, Alp HH, et al. Measuring Oxidative DNA Damage With 8-Hydroxy-2'-Deoxyguanosine Levels in Patients With Laryngeal Cancer. Ann Otol Rhinol Laryngol 2017;126:103-9.
• 9. He H, Zhao Y, Wang N, Zhang L, Wang C. 8-Hydroxy-2'-deoxyguanosine expression predicts outcome of esophageal cancer. Ann Diagn Pathol 2014;18:326-8.
• 10. Kumar A, Pant MC, Singh HS, Khandelwal S. Determinants of oxidative stress and DNA damage (8-OhdG) in squamous cell carcinoma of head and neck. Indian J Cancer 2012;49:309-15.
• 11. Aksoy A, Kurnaz SÇ. An investigation of oxidative stress and coenzyme Q10 levels in patients with head and neck squamous cell carcinomas. Eur Arch Otorhinolaryngol 2019;276:1197-204.
• 12. Basu S. Metabolism of 8-iso-prostaglandin F2alpha. FEBS Lett 1998;428:32-6.
• 13. Basu S. Fatty acid oxidation and isoprostanes: oxidative strain and oxidative stress. Prostaglandins Leukot Essent Fatty Acids 2010;82:219-25.
• 14. Dogan R, Meriç Hafiz A, Tugrul S, Ozturan O, Keskin S, Kocyigit A. Can Oxidative Stress Parameters Be Used as Biomarkers for the Discrimination of Malignant Head and Neck Tumors. J Craniofac Surg 2016;27:316-20.
• 15. Bozan N, Demir H, Gürsoy T, et al. Alterations in oxidative stress markers in laryngeal carcinoma patients. J Chin Med Assoc 2018;81:811-5.
• 16. Karaman E, Uzun H, Papila I, et al. Serum paraoxonase activity and oxidative DNA damage in patients with laryngeal squamous cell carcinoma. J Craniofac Surg 2010;21:1745-9.
• 17. Kaur J, Politis C, Jacobs R. Salivary 8-hydroxy-2-deoxyguanosine, malondialdehyde, vitamin C, and vitamin E in oral pre-cancer and cancer: diagnostic value and free radical mechanism of action. Clin Oral Investig 2016;20:315-9.
• 18. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 2006;160:1-40.
• 19. Marczynski B, Rozynek P, Kraus T, Schlösser S, Raithel HJ, Baur X. Levels of 8-hydroxy-2'-deoxyguanosine in DNA of white blood cells from workers highly exposed to asbestos in Germany. Mutat Res 2000;468:195-202.
• 20. Brys M, Morel A, Forma E, et al. Relationship of urinary isoprostanes to prostate cancer occurrence. Mol Cell Biochem 2013;372:149-53.
• 21. Gào X, Brenner H, Holleczek B, et al. Urinary 8-isoprostane levels and occurrence of lung, colorectal, prostate, breast and overall cancer: Results from a large, population-based cohort study with 14 years of follow-up. Free Radic Biol Med 2018;123:20-6.
• 22. Senghore T, Li YF, Sung FC, et al. Biomarkers of Oxidative Stress Associated with the Risk of Potentially Malignant Oral Disorders. Anticancer Res 2018;38:4661-6.
• 23. Asami S, Manabe H, Miyake J, et al. Cigarette smoking induces an increase in oxidative DNA damage, 8-hydroxydeoxyguanosine, in a central site of the human lung. Carcinogenesis 1997;18:1763-6.
• 24. Morrow JD, Frei B, Longmire AW, et al. Increase in circulating products of lipid peroxidation (F2-isoprostanes) in smokers. Smoking as a cause of oxidative damage. N Engl J Med 1995;332:1198-203.