Fluoxetine and Gundelia tournefortii L. Plant Extract in Rats Exposed to Chronic Immobilization Stress; Determination of Effect on Anxiety, Motor Activity, Kidney and Liver Tissues

Öz

This study was completed with the aim of investigating the effects of extracts obtained from Gundelia tournefortii L. and fluoxetine on anxiety, motor activity, biochemical and antioxidant parameters in rats exposed to chronic immobilization stress. The study included a total of 40 female Wistar albino rats with live weight of 200-220 g, randomly divided into groups of 8 rats with the study duration planned as 30 days. The groups created in the study were “control”, “physiologic serum + chronic immobilization”, “Gundelia tournefortii L. plant extract”, “Gundelia tournefortii L. plant extract + chronic immobilization” and “fluoxetine + chronic immobilization”. At the end of 30 days, all the groups had rotarod test (motor activity) and anxiety test (elevated plus maze) applied and after all the tests were completed the rats were sacrificed. At the end of the study, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), uric acid (UA), creatinine (CRE), total protein (TP) and albumin levels were determined. Additionally, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GR) activity and reduced glutathione (GSH) and malondialdehyde (MDA) levels were identified in liver and kidney tissue samples. In conclusion, administration of both Gundelia tournefortii L. plant extract and fluoxetine can be said to have positive effects on enzyme activities and MDA parameter value against experimentally-induced immobilization stress. Additionally, Gundelia tournefortii L. was not identified to have any effect on motor activity, but identified to have anxiolytic effect according to elevated plus maze test results.

Anahtar Kelimeler

• Chronic immobilization stress
• elevated plus maze
• fluoxetine
• Gundelia tournefortii L.
• rotarod

Kronik İmmobilizasyon Stresine Maruz Bırakılan Ratlarda Fluoksetin ve Gundelia tournefortii L. Bitki Ekstresinin; Anksiyete, Motor Aktivite, Böbrek ve Karaciğer Dokuları Üzerine Etkisinin Belirlenmesi

Öz

Bu çalışma, kronik immobilizasyon stresine maruz bırakılan ratlarda anksiyete, motor aktivite, biyokimyasal ve antioksidan parametre değerleri üzerine Gundelia tournefortii L. bitkisinden elde edilen ekstrelerin ve Fluoksetin’in etkilerinin incelenmesi amacıyla gerçekleştirilmiştir. Çalışmada canlı ağırlıkları 200-220 gr olan toplam 40 adet Wistar – albino ırkı dişi sıçan her grupta 8 sıçan olacak şekilde gruplara rastgele dağıtılmış ve çalışmanın süresi toplam 30 gün olarak planlanmıştır. Çalışmadaki gruplar; “Kontrol”, “Serum fizyolojik su + Kronik İmmobilizasyon”, “Gundelia tournefortii L. bitki ekstresi”, “Gundelia tournefortii L. bitki ektresi + Kronik immobilizasyon” ve “Fluoksetin + Kronik immobilizasyon stresi” şeklinde oluşturulmuştur. 30’uncu gün sonunda tüm gruplara rotarod testi (motor aktivite testi) ve anksiyete testi (yükseltilmiş artı labirent testi) uygulananarak tüm testler tamamlandıktan sonra uygulama sonunda sıçanlar sakrifiye edilmiştir. Çalışma sonunda aspartat aminotransferaz (AST), alanin aminotransferaz (ALT), alkalen fosfataz (ALP), ürik asit(UA), kreatinin (CRE), total protein (TP), albumin düzeyleri belirlenmiştir. Ayrıca, karaciğer ve böbrek dokusu örneklerinde katalaz (CAT), süperoksid dismutaz (SOD), glutatyon peroksidaz (GPx), glutatyon S-transferaz (GST), glutatyon redüktaz (GR) aktiviteleri ve redükte glutatyon (GSH) ile malondialdehit (MDA) düzeyleri tespit edilmiştir. Sonuç olarak, deneysel olarak oluşturulan immobilizasyon stresine karşı, hem Gundelia tournefortii L. bitki ekstresinin hem de Fluoksetin uygulamasının enzim aktiviteleri üzerine ve MDA parametre değeri üzerine olumlu etkiler sergilediği söylenebilir. Bununla birlikte, Gundelia tournefortii L. bitkisinin; motor aktivite üzerine herhangi bir etkisi olmadığı tespit edilirken, yükseltilmiş artı labirent testi sonuçlarına göre, anksiyolitik etkisi olduğu belirlenmiştir.

Anahtar Kelimeler

• Fluoksetin, Gundelia tournefortii L.
• kronik immobilizasyon stresi
• rotarod
• yükseltilmiş artı labirent

Kaynakça

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