Resveratrol Suppresses Cell Viability and Invasion in Pancreatic Cancer Cells

Yıl 2025, Cilt: 6 Sayı: 1, 22 - 27, 15.03.2025

Erhan Şahin

Öz

Introduction: Pancreatic cancer is a challenging disease to diagnose and treat due to its asymptomatic progression and high mortality rate. It is often identified at an advanced stage, where it typically metastasizes to other organs. To address these challenges, scientific studies explore various treatments, including natural and anticancer compounds like resveratrol. This study investigates the effects of resveratrol on pancreatic cancer cells.

Methods: The study used PANC-1 pancreatic cancer cells cultured in vitro. Cell viability in resveratrol-containing media was measured using the MTT assay. Safe, toxic, and IC50 doses for a 24-hour period were determined. Morphological changes were analyzed using hematoxylin-eosin staining. In invasion experiments, a wound-healing assay was performed by scraping cells in a 6-well plate and photographing their movement in resveratrol media.

Results: Resveratrol increased cell viability at concentrations up to 10 µM, showed similar viability to the control at 50 µM, and reduced viability below 50% at 200 µM. The IC50 dose was calculated as 189.5 µM. At concentrations above 100 µM, significant disruptions in nuclear and cytoplasmic structures were observed. Additionally, cell movement ceased at 100, 150, and 189 µM doses, showing dose-dependent inhibition of cell invasion (p<0.05).

Conclusions: Resveratrol, a naturally occurring compound found in many fruits and plants, demonstrated cytotoxic and anti-metastatic effects on PANC-1 cells. This study highlights its potential role in pancreatic cancer treatment, emphasizing the importance of continued research to combat this aggressive disease effectively.

Anahtar Kelimeler

PANC-1 cells, cell viability, anti-metastatic, resveratrol, anti-proliferative

Resveratrol, Pankreas Kanseri Hücrelerinde Hücre Canlılığını ve İnvazyonunu Baskılar

Öz

Giriş: Pankreas kanseri, asemptomatik ilerlemesi, teşhis ve tedavisinin zor olması nedeniyle yüksek ölüm oranlarına neden olan bir hastalıktır. Genellikle ileri bir aşamada tanımlanır ve bu nedenle başka organlara metastaz yapar. Bu hastalığın tedavisindeki zorluklar nedeniyle araştırmacılar bazı antikanser özelliği yüksek moleküllerin de pankreas kanserine etkisini araştırmışlardır. Çalışmamızda, bu moleküllerden biri olan resveratrolün pankreas kanseri hücrelerine olan potansiyel etkilerini araştırdık.

Yöntemler: Çalışmamızda, in vitro kültüre edilmiş PANC-1 pankreas kanseri hücreleri kullanılmıştır. Resveratrol içeren midyumlarda hücre canlılığı, MTT testi kullanılarak ölçülmüştür. Resveratrolün 24 saatlik güvenli, sitotoksik ve IC50 dozları belirlenmiştir. Morfolojik değişiklikler hematoksilin-eozin boyama kullanılarak analiz edilmiştir. İnvazyon deneylerinde, 6 kuyucuklu plakalarda yara iyileştirme testi gerçekleştirilmiştir.

Bulgular: Resveratrol 10 µM'a kadar hücre canlılığını arttırmış, 50 µM'da kontrole benzer canlılık göstermiş ve 200 µM'da hücre canlılığını %50'nin altına düşürmüştür. IC50 dozu 189,5 µM olarak hesaplanmıştır. 100 µM'nin üzerindeki konsantrasyonlarda, nükleer ve sitoplazmik yapılarda önemli bozulmalar gözlenmiştir. Ek olarak, resveratrol hücre hareketini 100, 150 ve 189 µM’larda durdurarak hücre hareketinde doza bağlı inhibisyon göstermiştir (p<0,05).

Sonuç: Bu çalışma ile kanser proliferasyonunu ve hareketini inhibe edici etkiye sahip bir bileşikle pankreas kanseri tedavisine katkıda bulunma amaçlanmıştır. Birçok meyve ve bitkide bulunan ve doğal olarak oluşan bir bileşik olan resveratrol, PANC-1 hücreleri üzerinde sitotoksik ve anti-metastatik etkiler göstermiştir. Çalışmamız sonuçlarına göre, bu alanda daha fazla derinlemesine çalışmalara ihtiyaç olduğu açıktır.

Anahtar Kelimeler

PANC-1 hücreleri, hücre canlılığı, anti-metastatik, resveratrol, anti-proliferatif

Kaynakça

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