EXOPOLYSACCHARIDE (EPS) ISOLATED FROM ENTEROCOCCUS FAECIUM D36 SHOWS ANTI-CANCER AND ANTI-INVASIVE ACTIVITY POTENTIAL VIA DOWN-REGULATION OF MUC5AC GENE ON HUMAN COLORECTAL ADENOCARCINOMA (CACO-2) CELLS

Yıl 2024, , 8 - 17, 30.01.2024

Burcugül Altuğ Tasa , Merih Kıvanç , Ayşe Tansu Koparal

https://doi.org/10.18036/estubtdc.1265111

Öz

To investigate the antiproliferative properties of exopolysaccharides (EPS) on human colorectal adenocarcinoma cell line (Caco-2) and the regulation of MUC5AC gene expression, the antiproliferative effect of EPS isolated from D36 strain was determined by MTT test and the regulation of MUC5AC gene expression was examined using Real-Time PCR. Enterococcus faecium D36 (E. faecium D36) were characterized by Ribotyping analysis. Some biochemical methodologies were preliminarily used to characterize the probiotic potential of E. faecium D36, including morphological, cultural, and physiological characteristics. EPS isolated from E. faecium D36 strain has an antiproliferative effect on Caco-2 cell line, and mucin gene (MUC5AC) expression levels decreased. These results suggest that EPS isolated from E. faecium D36 strain might be a source for a novel anticancer agent. Based on our results, it is believed that EPS obtained from E. faecium D36 can be used as a protective and therapeutic substance during the early stages of cancer, especially colon cancer. EPS affects colon cancer by reducing the invasion ability of cancer by decreasing MUC5AC expression. These findings are thought to shed light on future in vivo studies.

Anahtar Kelimeler

Colorectal cancer, Lactic acid bacteria, Enterococcus faecium, Exopolysaccharide, MUC5AC

Proje Numarası

1201F014

ENTEROCOCCUS FAECIUM D36'DAN İZOLE EDİLEN EKZOPOLİSAKKARİTİN (EPS), İNSAN KOLOREKTAL ADENOKARSİNOM (CACO-2) HÜCRELERİNDE MUC5AC GENİNİN REGÜLASYONU İLE ANTİ-KANSER VE ANTİ-İNVAZIV AKTİVİTESİ

Öz

Ekzopolisakkaritlerin (EPS) insan kolorektal adenokarsinom hücre hattı (Caco-2) üzerindeki antiproliferatif özelliklerini ve MUC5AC gen ekspresyonunun düzenlenmesini araştırmak için, D36 suşundan izole edilen EPS'nin antiproliferatif etkisi MTT testi ile belirlenmiş ve MUC5AC gen ekspresyonunun düzenlenmesi Real-Time PCR kullanılarak incelenmiştir. Enterococcus faecium D36 (E. faecium D36) Ribotipleme analizi ile karakterize edilmiştir. E. faecium D36'nın probiyotik potansiyelini karakterize etmek için morfolojik, kültürel ve fizyolojik özellikler de dahil olmak üzere bazı biyokimyasal metodolojiler ön hazırlık olarak kullanılmıştır. E. faecium D36 suşundan izole edilen EPS, Caco-2 hücre hattı üzerinde antiproliferatif bir etkiye sahiptir ve MUC5AC gen ekspresyon seviyeleri azalmıştır. Bu sonuçlar, E. faecium D36 suşundan izole edilen EPS'nin yeni bir antikanser ajan için bir kaynak olabileceğini göstermektedir. Sonuçlarımıza dayanarak, E. faecium D36'dan elde edilen EPS'nin kanserin, özellikle de kolon kanserinin erken evrelerinde koruyucu ve tedavi edici bir madde olarak kullanılabileceğine inanılmaktadır. EPS, müsin geni (MUC5AC) ekspresyonunu azaltarak kanserin invazyon yeteneğini azaltarak kolon kanserini etkilemektedir. Bu bulguların gelecekte yapılacak in vivo çalışmalara ışık tutacağı düşünülmektedir

Anahtar Kelimeler

Enterococcus faecium, kolorektal kanser, laktik asit bakterileri, Enterococcus faecium, eksopolisakkarit, MUC5AC

Destekleyen Kurum

Anadolu Üniversitesi

Proje Numarası

1201F014

Teşekkür

Tez çalışmalarıma mali destek sağlayan Eskişehir Teknik Üniversitesi ve Anadolu Üniversitesi Rektörlüğü’ne ve Bilimsel Araştırma Projeleri Koordinasyon Birimine teşekkürlerimi sunarım.

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