THE PHARMACEUTICAL BOTANICAL STUDIES ON THE ENDEMIC Asperula pestalozzae Boiss. (RUBIACEAE)

Öz

In this study, endemic Asperula pestalozzae Boiss. (Rubiaceae) was investigated in aspects of pharmaceutical botany. The morphological, anatomical, micromorphological, palynological, and chemical (antioxidant activity and total amount of phenolic substance) characteristics were reported in detail for the first time. Expanded descriptions and images of A. pestalozzae were given. The anatomical description was presented in detail and supported by photographs. The stem is usually angular or orbicular shaped and the leaf is monofacial. Its trichome and pollen micromorphology were examined with a scanning electron microscope (SEM). The pollen grains of A. pestalozzae are monad, radial symmetry, isopolar, and hexacolpate (sometimes heptacolpate). DPPH and ABTS radicals were used to determine antioxidant activity. The DPPH 50% inhibition concentration (IC50) value was found to be 0.0011±0.0002 mg/ml for standard gallic acid and 0.134±0.017 mg/ml for A. pestalozzae extract. 0.1 mg/ml extract calculated 0.022±0.36 Mm TEAC activity; 1.437±0.51 mM TEAC activity was determined in 10 mg/ml extract. The total amount of phenolic substances was calculated as 53 mg gallic acid equivalent (GAE).

Anahtar Kelimeler

• Anatomy
• antioxidant activity
• Asperula
• morphology
• palynology

ENDEMİK Asperula pestalozzae Boiss. (RUBIACEAE) ÜZERİNDE FARMASÖTİK BOTANİK ARAŞTIRMALAR

Öz

Bu çalışmada endemik Asperula pestalozzae Boiss. (Rubiaceae) farmasötik botanik açısından araştırıldı. Morfolojik, anatomik, mikromorfolojik, palinolojik ve kimyasal (antioksidan aktivite ve toplam fenolik madde miktarı) özellikleri ilk kez ayrıntılı olarak rapor edildi. A. pestalozzae'nin genişletilmiş açıklamaları ve görüntüleri verildi. Anatomik açıklama ayrıntılı olarak sunuldu ve fotoğraflarla desteklendi. Gövde genellikle köşeli veya dairesel şekillidir ve yaprak tek yüzlüdür. Tüy ve polen mikromorfolojisi taramalı elektron mikroskobu (SEM) ile incelendi. A. pestalozzae'nin polen taneleri monad, radyal simetri, izopolar ve heksakolpattır (bazen heptakolpattır). Antioksidan aktiviteyi belirlemek için DPPH ve ABTS radikalleri kullanıldı. DPPH %50 inhibisyon konsantrasyonu (IC50) değeri standart gallik asit için 0,0011±0,0002 mg/ml, A. pestalozzae ekstraktı için 0,134±0,017 mg/ml olarak bulunmuştur. 0,1 mg/ml ekstraktta 0,022±0,36 Mm TEAC aktivitesi; 10 mg/ml ekstraktta 1.437±0.51 mM TEAC aktivitesi belirlendi. Toplam fenolik maddelerin miktarı 53 mg gallik asit eşdeğeri (GAE) olarak hesaplandı.

Anahtar Kelimeler

• anatomi
• antioksidan aktivite
• Asperula
• morfoloji
• palinoloji

Kaynakça

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