TUZ STRESİ KOŞULLARINDA SALİSİLİK ASİDİN ZAMANA BAĞLI UYGULANMASININ ARPA (Hordeum vulgare L.) KÖKLERİNİN ANTİOKSİDAN SAVUNMA SİSTEMİ ÜZERİNE ETKİLERİ

Yıl 2019, Cilt: 8 Sayı: 1, 69 - 84, 01.01.2019

Hülya Torun Faik Ahmet Ayaz

Öz

Toprak tuzluluğu önemli ürün kayıplarına neden olan başlıca abiyotik stres tiplerinden biridir. Tuzluluk stresi esnasında bitkide geliştirilen tolerans mekanizmalarını anlayabilmek, ürün verim ve kalitesindeki ciddi kayıpları azaltan tolerant çeşitlerin seçimi ve ıslahında önem arz etmektedir. Salisilik asit (SA), bitkilerde tuz stresi dahil pek çok abiyotik streste fizyolojik ve biyokimyasal süreçleri etkileyen önemli bir sinyal moleküldür. Bu çalışmada, SA’nın tuz stresi öncesinde ve stres süresince uygulanmasının, arpa (Hordeum vulgare L. cv. Kalaycı, Erginel, Akhisar) çeşitlerinin köklerinde büyüme parametreleri, prolin miktarı ve antioksidan savunma sistemi enzimlerinin (süperoksit dismutaz (SOD), peroksidaz (POX), katalaz (CAT), askorbat peroksidaz (APX), glutatyon redüktaz (GR)) aktivitelerini nasıl değiştirdiği incelenmiştir. Tuz stresi, arpanın her üç çeşidinde de büyüme parametrelerinde azalmaya neden olmuştur. Strese karşı en tolerant çeşit Kalaycı, en hassas ise Akhisar olarak belirlenmiştir. Ayrıca, SA’nın uygulama süresinin antioksidan savunma sistemi üzerindeki etkisi ortaya konulmuştur. Sonuç olarak, SA antioksidan savunma sistemini uyararak tuz stresinin sebep olduğu oksidatif hasarı azaltmıştır. Bu etki, SA’nın uygulama zamanına ve türün çeşitlerine göre farklılık göstermiştir. Özellikle stres öncesi uygulanan SA, tuz stresine karşı daha yüksek tolerans göstermiş ve çeşitler arasında bu etki daha çok Kalaycı çeşidinde gözlenmiştir.

Anahtar Kelimeler

Antioksidan enzim, arpa, kök, salisilik asit, tuz stresi

EFFECTS OF TIME COURSE SALICYLIC ACID ON THE ANTIOXIDANT DEFENSE SYSTEM IN BARLEY ROOTS UNDER SALT STRESS

Öz

Soil salinity is one of the major abiotic stress types causing significant crop losses. To understand tolerance mechanisms occuring in plants during salinity stress is significant for choosing and improving tolerant species decreasing serious loosings in yield and quality in crops. Salicylic acid (SA) is an important signalling molecule affecting physiological and biochemical processes in many abiotic stresses including salt stress. In this study, how altered the growth parameters, proline amount, and the activities of antioxidant defense system enzymes (superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX),glutathione reductase (GR)) in roots of barley (Hordeum vulgare L. cv. Kalaycı, Erginel, Akhisar) cultivars with the application of pre- and simultaneous treatment of SA was investigated. Salt stress caused a decrease in growth parameters in all cultivars of barley. It was identified that Kalaycı was the most tolerant and Akhisar the most sensitive genotype against the stress. Moreover, the effect of time-dependent application of SA on the antioxidant defense system has been established. As a result, SA reduced the salt-induced oxidative damage caused by salt stress by stimulating the antioxidant defense system. This effect differed according to the application time of the SA and genotypes. Especially pretreatment of SA displayed higher tolerance to salt stress and this effect was more observed in Kalaycı among cultivars.

Anahtar Kelimeler

Antioxidant enzyme, barley, root, salicylic acid, salt stress

Kaynakça

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